S-nitrosothiol-terminated Pluronic F127: Influence of microstructure on nitric oxide release
(2020) In Journal of Colloid and Interface Science 576. p.457-467- Abstract
- Hypothesis: Nitric oxide (NO)-releasing Pluronic F127 hydrogels (F127) containing dissolved S-nitrosothiols or pendant N-diazeniumdiolate (NONOate) groups have been described. The NO charging of these hydrogels is usually limited by their low stability or disruption of the micellar packing. S-nitrosothiol-terminated F127 may emerge as a new strategy for allowing NO delivery at different rates in biomedical applications.
Experiments: Terminal hydroxyl groups of F127 were esterified and reduced to produce F127-mercaptopropionate (HS-F127-SH), which was subsequently S-nitrosated to generate S-nitrosothiolterminated F127 (ONS-F127-SNO). Micro-differential scanning calorimetry, 1H NMR spin-spin relaxation (T2), temperature-dependent... (More) - Hypothesis: Nitric oxide (NO)-releasing Pluronic F127 hydrogels (F127) containing dissolved S-nitrosothiols or pendant N-diazeniumdiolate (NONOate) groups have been described. The NO charging of these hydrogels is usually limited by their low stability or disruption of the micellar packing. S-nitrosothiol-terminated F127 may emerge as a new strategy for allowing NO delivery at different rates in biomedical applications.
Experiments: Terminal hydroxyl groups of F127 were esterified and reduced to produce F127-mercaptopropionate (HS-F127-SH), which was subsequently S-nitrosated to generate S-nitrosothiolterminated F127 (ONS-F127-SNO). Micro-differential scanning calorimetry, 1H NMR spin-spin relaxation (T2), temperature-dependent small-angle X-ray scattering, and cryo-transmission electron microscopy, were used to determine the micellar packing structure, while real-time chemiluminescence NO detection and UV–Vis spectrophotometry were used to evaluate the kinetics of NO release.
Findings: HS-F127-SH micellization and gelation processes were analogous to native F127, however, with a decreased short-range ordering of the micelles. ONS-F127-SNO hydrogels released NO thorough a preferentially intramicellar SNO dimerization reaction. Increasing ONS-F127-SNO concentration reduces the rate of SNO dimerization and increases the overall rate of NO release to the gas phase, opening up new possibilities for tailoring NO delivery from F127-based hydrogels. (Less)
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https://lup.lub.lu.se/record/49d4d850-952c-4bac-b223-bed2ef71ccdc
- author
- Pichet, Guilherme F. ; Da Silva, Laura C.E. ; Giglio, Leonardo P. ; Plivelic, Tomás LU and de Oliveira, Marcelo G.
- organization
- publishing date
- 2020-05-19
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Pluronic F127, Hydrogel, Reactive micelles, Microstructure, Nitric oxide, S-nitrosothiol, SAXS, Cryo TEM
- in
- Journal of Colloid and Interface Science
- volume
- 576
- pages
- 11 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85085235617
- pmid:32470853
- ISSN
- 0021-9797
- DOI
- 10.1016/j.jcis.2020.05.049
- language
- English
- LU publication?
- yes
- id
- 49d4d850-952c-4bac-b223-bed2ef71ccdc
- date added to LUP
- 2020-06-06 08:33:33
- date last changed
- 2022-04-18 22:38:28
@article{49d4d850-952c-4bac-b223-bed2ef71ccdc, abstract = {{Hypothesis: Nitric oxide (NO)-releasing Pluronic F127 hydrogels (F127) containing dissolved S-nitrosothiols or pendant N-diazeniumdiolate (NONOate) groups have been described. The NO charging of these hydrogels is usually limited by their low stability or disruption of the micellar packing. S-nitrosothiol-terminated F127 may emerge as a new strategy for allowing NO delivery at different rates in biomedical applications.<br/>Experiments: Terminal hydroxyl groups of F127 were esterified and reduced to produce F127-mercaptopropionate (HS-F127-SH), which was subsequently S-nitrosated to generate S-nitrosothiolterminated F127 (ONS-F127-SNO). Micro-differential scanning calorimetry, 1H NMR spin-spin relaxation (T2), temperature-dependent small-angle X-ray scattering, and cryo-transmission electron microscopy, were used to determine the micellar packing structure, while real-time chemiluminescence NO detection and UV–Vis spectrophotometry were used to evaluate the kinetics of NO release.<br/>Findings: HS-F127-SH micellization and gelation processes were analogous to native F127, however, with a decreased short-range ordering of the micelles. ONS-F127-SNO hydrogels released NO thorough a preferentially intramicellar SNO dimerization reaction. Increasing ONS-F127-SNO concentration reduces the rate of SNO dimerization and increases the overall rate of NO release to the gas phase, opening up new possibilities for tailoring NO delivery from F127-based hydrogels.}}, author = {{Pichet, Guilherme F. and Da Silva, Laura C.E. and Giglio, Leonardo P. and Plivelic, Tomás and de Oliveira, Marcelo G.}}, issn = {{0021-9797}}, keywords = {{Pluronic F127; Hydrogel; Reactive micelles; Microstructure; Nitric oxide; S-nitrosothiol; SAXS; Cryo TEM}}, language = {{eng}}, month = {{05}}, pages = {{457--467}}, publisher = {{Elsevier}}, series = {{Journal of Colloid and Interface Science}}, title = {{S-nitrosothiol-terminated Pluronic F127: Influence of microstructure on nitric oxide release}}, url = {{http://dx.doi.org/10.1016/j.jcis.2020.05.049}}, doi = {{10.1016/j.jcis.2020.05.049}}, volume = {{576}}, year = {{2020}}, }