Elemental and Structural Characterization of Heterotopic Ossification during Achilles Tendon Healing Provides New Insights on the Formation Process
(2024) In ACS Biomaterials Science and Engineering 10(8). p.4938-4946- Abstract
Heterotopic ossification (HO) in tendons can lead to increased pain and poor tendon function. Although it is believed to share some characteristics with bone, the structural and elemental compositions of HO deposits have not been fully elucidated. This study utilizes a multimodal and multiscale approach for structural and elemental characterization of HO deposits in healing rat Achilles tendons at 3, 6, 12, 16, and 20 weeks post transection. The microscale tomography and scanning electron microscopy results indicate increased mineral density and Ca/P ratio in the maturing HO deposits (12 and 20 weeks), when compared to the early time points (3 weeks). Visually, the mature HO deposits present microstructures similar to calcaneal bone.... (More)
Heterotopic ossification (HO) in tendons can lead to increased pain and poor tendon function. Although it is believed to share some characteristics with bone, the structural and elemental compositions of HO deposits have not been fully elucidated. This study utilizes a multimodal and multiscale approach for structural and elemental characterization of HO deposits in healing rat Achilles tendons at 3, 6, 12, 16, and 20 weeks post transection. The microscale tomography and scanning electron microscopy results indicate increased mineral density and Ca/P ratio in the maturing HO deposits (12 and 20 weeks), when compared to the early time points (3 weeks). Visually, the mature HO deposits present microstructures similar to calcaneal bone. Through synchrotron-based X-ray scattering and fluorescence, the hydroxyapatite (HA) crystallites are shorter along the c-axis and become larger in the ab-plane with increasing healing time, while the HA crystal thickness remains within the reference values for bone. At the mineralization boundary, the overlap between high levels of calcium and prominent crystallite formation was outlined by the presence of zinc and iron. In the mature HO deposits, the calcium content was highest, and zinc was more present internally, which could be indicative of HO deposit remodeling. This study emphasizes the structural and elemental similarities between the calcaneal bone and HO deposits.
(Less)
- author
- Sharma, Kunal
LU
; Silva Barreto, Isabella
LU
; Dejea, Hector LU ; Hammerman, Malin LU ; Appel, Christian ; Geraki, Kalotina ; Eliasson, Pernilla ; Pierantoni, Maria LU
and Isaksson, Hanna LU
- organization
- publishing date
- 2024-08
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- small animal model, small-angle X-ray scattering, tissue characterization, wide-angle X-ray scattering, X-ray fluorescence
- in
- ACS Biomaterials Science and Engineering
- volume
- 10
- issue
- 8
- pages
- 9 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:39042709
- scopus:85199430807
- ISSN
- 2373-9878
- DOI
- 10.1021/acsbiomaterials.4c00935
- language
- English
- LU publication?
- yes
- id
- 4ae9be79-2bfb-47ec-a90c-67734714d16e
- date added to LUP
- 2024-09-06 14:30:46
- date last changed
- 2024-09-07 03:00:05
@article{4ae9be79-2bfb-47ec-a90c-67734714d16e, abstract = {{<p>Heterotopic ossification (HO) in tendons can lead to increased pain and poor tendon function. Although it is believed to share some characteristics with bone, the structural and elemental compositions of HO deposits have not been fully elucidated. This study utilizes a multimodal and multiscale approach for structural and elemental characterization of HO deposits in healing rat Achilles tendons at 3, 6, 12, 16, and 20 weeks post transection. The microscale tomography and scanning electron microscopy results indicate increased mineral density and Ca/P ratio in the maturing HO deposits (12 and 20 weeks), when compared to the early time points (3 weeks). Visually, the mature HO deposits present microstructures similar to calcaneal bone. Through synchrotron-based X-ray scattering and fluorescence, the hydroxyapatite (HA) crystallites are shorter along the c-axis and become larger in the ab-plane with increasing healing time, while the HA crystal thickness remains within the reference values for bone. At the mineralization boundary, the overlap between high levels of calcium and prominent crystallite formation was outlined by the presence of zinc and iron. In the mature HO deposits, the calcium content was highest, and zinc was more present internally, which could be indicative of HO deposit remodeling. This study emphasizes the structural and elemental similarities between the calcaneal bone and HO deposits.</p>}}, author = {{Sharma, Kunal and Silva Barreto, Isabella and Dejea, Hector and Hammerman, Malin and Appel, Christian and Geraki, Kalotina and Eliasson, Pernilla and Pierantoni, Maria and Isaksson, Hanna}}, issn = {{2373-9878}}, keywords = {{small animal model; small-angle X-ray scattering; tissue characterization; wide-angle X-ray scattering; X-ray fluorescence}}, language = {{eng}}, number = {{8}}, pages = {{4938--4946}}, publisher = {{The American Chemical Society (ACS)}}, series = {{ACS Biomaterials Science and Engineering}}, title = {{Elemental and Structural Characterization of Heterotopic Ossification during Achilles Tendon Healing Provides New Insights on the Formation Process}}, url = {{http://dx.doi.org/10.1021/acsbiomaterials.4c00935}}, doi = {{10.1021/acsbiomaterials.4c00935}}, volume = {{10}}, year = {{2024}}, }