Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Elemental and Structural Characterization of Heterotopic Ossification during Achilles Tendon Healing Provides New Insights on the Formation Process

Sharma, Kunal LU ; Silva Barreto, Isabella LU orcid ; Dejea, Hector LU ; Hammerman, Malin LU ; Appel, Christian ; Geraki, Kalotina ; Eliasson, Pernilla ; Pierantoni, Maria LU orcid and Isaksson, Hanna LU orcid (2024) In ACS Biomaterials Science and Engineering 10(8). p.4938-4946
Abstract

Heterotopic ossification (HO) in tendons can lead to increased pain and poor tendon function. Although it is believed to share some characteristics with bone, the structural and elemental compositions of HO deposits have not been fully elucidated. This study utilizes a multimodal and multiscale approach for structural and elemental characterization of HO deposits in healing rat Achilles tendons at 3, 6, 12, 16, and 20 weeks post transection. The microscale tomography and scanning electron microscopy results indicate increased mineral density and Ca/P ratio in the maturing HO deposits (12 and 20 weeks), when compared to the early time points (3 weeks). Visually, the mature HO deposits present microstructures similar to calcaneal bone.... (More)

Heterotopic ossification (HO) in tendons can lead to increased pain and poor tendon function. Although it is believed to share some characteristics with bone, the structural and elemental compositions of HO deposits have not been fully elucidated. This study utilizes a multimodal and multiscale approach for structural and elemental characterization of HO deposits in healing rat Achilles tendons at 3, 6, 12, 16, and 20 weeks post transection. The microscale tomography and scanning electron microscopy results indicate increased mineral density and Ca/P ratio in the maturing HO deposits (12 and 20 weeks), when compared to the early time points (3 weeks). Visually, the mature HO deposits present microstructures similar to calcaneal bone. Through synchrotron-based X-ray scattering and fluorescence, the hydroxyapatite (HA) crystallites are shorter along the c-axis and become larger in the ab-plane with increasing healing time, while the HA crystal thickness remains within the reference values for bone. At the mineralization boundary, the overlap between high levels of calcium and prominent crystallite formation was outlined by the presence of zinc and iron. In the mature HO deposits, the calcium content was highest, and zinc was more present internally, which could be indicative of HO deposit remodeling. This study emphasizes the structural and elemental similarities between the calcaneal bone and HO deposits.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
small animal model, small-angle X-ray scattering, tissue characterization, wide-angle X-ray scattering, X-ray fluorescence
in
ACS Biomaterials Science and Engineering
volume
10
issue
8
pages
9 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:39042709
  • scopus:85199430807
ISSN
2373-9878
DOI
10.1021/acsbiomaterials.4c00935
language
English
LU publication?
yes
id
4ae9be79-2bfb-47ec-a90c-67734714d16e
date added to LUP
2024-09-06 14:30:46
date last changed
2024-09-07 03:00:05
@article{4ae9be79-2bfb-47ec-a90c-67734714d16e,
  abstract     = {{<p>Heterotopic ossification (HO) in tendons can lead to increased pain and poor tendon function. Although it is believed to share some characteristics with bone, the structural and elemental compositions of HO deposits have not been fully elucidated. This study utilizes a multimodal and multiscale approach for structural and elemental characterization of HO deposits in healing rat Achilles tendons at 3, 6, 12, 16, and 20 weeks post transection. The microscale tomography and scanning electron microscopy results indicate increased mineral density and Ca/P ratio in the maturing HO deposits (12 and 20 weeks), when compared to the early time points (3 weeks). Visually, the mature HO deposits present microstructures similar to calcaneal bone. Through synchrotron-based X-ray scattering and fluorescence, the hydroxyapatite (HA) crystallites are shorter along the c-axis and become larger in the ab-plane with increasing healing time, while the HA crystal thickness remains within the reference values for bone. At the mineralization boundary, the overlap between high levels of calcium and prominent crystallite formation was outlined by the presence of zinc and iron. In the mature HO deposits, the calcium content was highest, and zinc was more present internally, which could be indicative of HO deposit remodeling. This study emphasizes the structural and elemental similarities between the calcaneal bone and HO deposits.</p>}},
  author       = {{Sharma, Kunal and Silva Barreto, Isabella and Dejea, Hector and Hammerman, Malin and Appel, Christian and Geraki, Kalotina and Eliasson, Pernilla and Pierantoni, Maria and Isaksson, Hanna}},
  issn         = {{2373-9878}},
  keywords     = {{small animal model; small-angle X-ray scattering; tissue characterization; wide-angle X-ray scattering; X-ray fluorescence}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{4938--4946}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{ACS Biomaterials Science and Engineering}},
  title        = {{Elemental and Structural Characterization of Heterotopic Ossification during Achilles Tendon Healing Provides New Insights on the Formation Process}},
  url          = {{http://dx.doi.org/10.1021/acsbiomaterials.4c00935}},
  doi          = {{10.1021/acsbiomaterials.4c00935}},
  volume       = {{10}},
  year         = {{2024}},
}