Laccase production by Galerina sp. and its application in dye decolorization
(2014) In Journal of Yeast and Fungal Research 5(2). p.13-22- Abstract
- The laccase producing fungus HC1 was isolated from a sample collected in the Bolivian Amazon region. Analysis of 5.8S and 28S rDNA, and internal transcribed spacer 1 and 2 sequences revealed that isolate HC1 belongs to genus Galerina. High production of laccase was achieved in basal salt medium supplemented with 30 g L-1 glucose, 10 g L-1 yeast extract and 0.01 g L-1 copper sulphate. The enzyme production was further improved by addition of 2,5-xylidine, α-benzoin oxime and p-coumaric acid, but strongly repressed by sinapyl alcohol, 3-(dimethylamino)benzoic acid, hydroquinone, 1-naphthale-neacetic acid, 2,6-dichlorophenol and 3-methyl-2-benzothiazolinone hydrazone. Cultivation under ... (More)
- The laccase producing fungus HC1 was isolated from a sample collected in the Bolivian Amazon region. Analysis of 5.8S and 28S rDNA, and internal transcribed spacer 1 and 2 sequences revealed that isolate HC1 belongs to genus Galerina. High production of laccase was achieved in basal salt medium supplemented with 30 g L-1 glucose, 10 g L-1 yeast extract and 0.01 g L-1 copper sulphate. The enzyme production was further improved by addition of 2,5-xylidine, α-benzoin oxime and p-coumaric acid, but strongly repressed by sinapyl alcohol, 3-(dimethylamino)benzoic acid, hydroquinone, 1-naphthale-neacetic acid, 2,6-dichlorophenol and 3-methyl-2-benzothiazolinone hydrazone. Cultivation under optimum conditions in the presence of 2 mmol L-1 2,5-xylidine and 0.01 g L-1 copper sulphate, resulted in the enzyme yield of over 26 000 U L-1. Galerina sp. HC1 could also produce laccase in media composed of orange peels both in submerged- and solid-state fermentations. The laccase (0.5 U ml-1) from Galerinasp. was able to decolorize over 60% of 35 μmol L-1 Congo red in 2 h in the presence of 2,2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt as mediator, and 65% of 30 μmol L-1 Coomassie Brilliant Blue G-250 in 5 min using syringaldehyde as mediator. The laccase was immobilized on cross-linked chitosan and used efficiently to decolorize the dyes. (Less)
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https://lup.lub.lu.se/record/4b06e6b5-0791-4e5b-ac37-3f5840998157
- author
- Mendoza, Laura LU ; Ibrahim, Victor LU ; Alvarez, Maria Teresa ; Hatti-Kaul, Rajni LU and Mamo, Gashaw LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Yeast and Fungal Research
- volume
- 5
- issue
- 2
- pages
- 13 - 22
- DOI
- 10.5897/JYFR12.025
- language
- English
- LU publication?
- yes
- id
- 4b06e6b5-0791-4e5b-ac37-3f5840998157
- date added to LUP
- 2019-06-28 01:14:51
- date last changed
- 2019-06-28 10:53:04
@article{4b06e6b5-0791-4e5b-ac37-3f5840998157, abstract = {{The laccase producing fungus HC1 was isolated from a sample collected in the Bolivian Amazon region. Analysis of 5.8S and 28S rDNA, and internal transcribed spacer 1 and 2 sequences revealed that isolate HC1 belongs to genus Galerina. High production of laccase was achieved in basal salt medium supplemented with 30 g L-1 glucose, 10 g L-1 yeast extract and 0.01 g L-1 copper sulphate. The enzyme production was further improved by addition of 2,5-xylidine, α-benzoin oxime and p-coumaric acid, but strongly repressed by sinapyl alcohol, 3-(dimethylamino)benzoic acid, hydroquinone, 1-naphthale-neacetic acid, 2,6-dichlorophenol and 3-methyl-2-benzothiazolinone hydrazone. Cultivation under optimum conditions in the presence of 2 mmol L-1 2,5-xylidine and 0.01 g L-1 copper sulphate, resulted in the enzyme yield of over 26 000 U L-1. Galerina sp. HC1 could also produce laccase in media composed of orange peels both in submerged- and solid-state fermentations. The laccase (0.5 U ml-1) from Galerinasp. was able to decolorize over 60% of 35 μmol L-1 Congo red in 2 h in the presence of 2,2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt as mediator, and 65% of 30 μmol L-1 Coomassie Brilliant Blue G-250 in 5 min using syringaldehyde as mediator. The laccase was immobilized on cross-linked chitosan and used efficiently to decolorize the dyes.}}, author = {{Mendoza, Laura and Ibrahim, Victor and Alvarez, Maria Teresa and Hatti-Kaul, Rajni and Mamo, Gashaw}}, language = {{eng}}, number = {{2}}, pages = {{13--22}}, series = {{Journal of Yeast and Fungal Research}}, title = {{Laccase production by Galerina sp. and its application in dye decolorization}}, url = {{http://dx.doi.org/10.5897/JYFR12.025}}, doi = {{10.5897/JYFR12.025}}, volume = {{5}}, year = {{2014}}, }