Miniaturized thermal biosensors
(1996) In Advances in Molecular and Cell Biology 15. p.499-505- Abstract
- A summary of our efforts to develop miniaturized biosensors based on the enzyme thermistor is presented. Three constructions are described. The work focuses on the measurement of glucose in whole blood.
The first biosensor was used to analyze concentrated and tenfold diluted blood samples. The glucose concentration obtained using this construction correlated well with the reference method.
Using the second biosensor, the response time could be reduced from 3 minutes down to 30 seconds using a sample volume of 1 μl. The relative standard deviation for 100 blood samples (3.8 tnM) was 3.7%.
Micromachining technology was used to construct the third thermal biosensor on a silicon chip. Enzymes were immobilized... (More) - A summary of our efforts to develop miniaturized biosensors based on the enzyme thermistor is presented. Three constructions are described. The work focuses on the measurement of glucose in whole blood.
The first biosensor was used to analyze concentrated and tenfold diluted blood samples. The glucose concentration obtained using this construction correlated well with the reference method.
Using the second biosensor, the response time could be reduced from 3 minutes down to 30 seconds using a sample volume of 1 μl. The relative standard deviation for 100 blood samples (3.8 tnM) was 3.7%.
Micromachining technology was used to construct the third thermal biosensor on a silicon chip. Enzymes were immobilized directly onto the 30 parallel flow channels. Injection of 200 samples containing 10 mM hydrogen peroxide gave a relative standard deviation of 3%. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4c209487-54a6-491a-bf80-380e3c89bdf8
- author
- Hedberg, U. ; Xie, B. LU and Danielsson, B. LU
- organization
- publishing date
- 1996
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- Biochemical Technology
- series title
- Advances in Molecular and Cell Biology
- editor
- Bittar, Edward ; Danielsson, Bengt and Bülow, Leif
- volume
- 15
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:77956726724
- ISSN
- 1569-2558
- ISBN
- 0762301147
- 9780762301140
- DOI
- 10.1016/S1569-2558(08)60130-7
- language
- English
- LU publication?
- yes
- id
- 4c209487-54a6-491a-bf80-380e3c89bdf8
- date added to LUP
- 2021-10-27 21:13:56
- date last changed
- 2022-02-02 00:55:13
@inbook{4c209487-54a6-491a-bf80-380e3c89bdf8,
abstract = {{A summary of our efforts to develop miniaturized biosensors based on the enzyme thermistor is presented. Three constructions are described. The work focuses on the measurement of glucose in whole blood.<br/><br/>The first biosensor was used to analyze concentrated and tenfold diluted blood samples. The glucose concentration obtained using this construction correlated well with the reference method.<br/><br/>Using the second biosensor, the response time could be reduced from 3 minutes down to 30 seconds using a sample volume of 1 μl. The relative standard deviation for 100 blood samples (3.8 tnM) was 3.7%.<br/><br/>Micromachining technology was used to construct the third thermal biosensor on a silicon chip. Enzymes were immobilized directly onto the 30 parallel flow channels. Injection of 200 samples containing 10 mM hydrogen peroxide gave a relative standard deviation of 3%.}},
author = {{Hedberg, U. and Xie, B. and Danielsson, B.}},
booktitle = {{Biochemical Technology}},
editor = {{Bittar, Edward and Danielsson, Bengt and Bülow, Leif}},
isbn = {{0762301147}},
issn = {{1569-2558}},
language = {{eng}},
pages = {{499--505}},
publisher = {{Elsevier}},
series = {{Advances in Molecular and Cell Biology}},
title = {{Miniaturized thermal biosensors}},
url = {{http://dx.doi.org/10.1016/S1569-2558(08)60130-7}},
doi = {{10.1016/S1569-2558(08)60130-7}},
volume = {{15}},
year = {{1996}},
}