An H-2 alloantiserum preserves β-cell function in mice made diabetic by low-dose streptozotocin
(1986) In Diabetes 35(5). p.570-573- Abstract
The pancreatic β-cell mass and function in C57BL/KsJ mice is markedly reduced the day after the last injection of five daily injections of a subdiabetogenic, 40 mg/kg, dose of streptozotocin (STZ). In this study, we prepared an H-2 alloantiserum by injecting C57BL/6J mice (H-2b) with spleen lymphocytes from C57BL/KsJ (H-2(d)) mice. The alloantiserum given on five consecutive days, 5 h before each injection of STZ, did not prevent the initial β-cytotoxic effect of STZ detected by perfusion of the pancreas and subsequent morphometric analysis of in situ dithizone-perfused pancreas. However, 12 days after the first injection of STZ, total insulin release in response to D-glucose, total pancreatic insulin, and pancreatic glucagon... (More)
The pancreatic β-cell mass and function in C57BL/KsJ mice is markedly reduced the day after the last injection of five daily injections of a subdiabetogenic, 40 mg/kg, dose of streptozotocin (STZ). In this study, we prepared an H-2 alloantiserum by injecting C57BL/6J mice (H-2b) with spleen lymphocytes from C57BL/KsJ (H-2(d)) mice. The alloantiserum given on five consecutive days, 5 h before each injection of STZ, did not prevent the initial β-cytotoxic effect of STZ detected by perfusion of the pancreas and subsequent morphometric analysis of in situ dithizone-perfused pancreas. However, 12 days after the first injection of STZ, total insulin release in response to D-glucose, total pancreatic insulin, and pancreatic glucagon was greater in the alloantiserum-treated mice compared with controls receiving normal mouse serum. It is concluded that an H-2 alloantiserum may protect the function and amounts of β-cells remaining after the initial five low-dose STZ injections.
(Less)
- author
- Bonnevie-Nielsen, V. and Lernmark, A. LU
- publishing date
- 1986-01-01
- type
- Contribution to journal
- publication status
- published
- in
- Diabetes
- volume
- 35
- issue
- 5
- pages
- 4 pages
- publisher
- American Diabetes Association Inc.
- external identifiers
-
- scopus:0022548796
- pmid:3514332
- ISSN
- 0012-1797
- language
- English
- LU publication?
- no
- id
- 4d7e73c0-3d94-4144-8d7c-d0dc018bb705
- date added to LUP
- 2019-09-16 12:34:44
- date last changed
- 2024-03-13 08:20:56
@article{4d7e73c0-3d94-4144-8d7c-d0dc018bb705, abstract = {{<p>The pancreatic β-cell mass and function in C57BL/KsJ mice is markedly reduced the day after the last injection of five daily injections of a subdiabetogenic, 40 mg/kg, dose of streptozotocin (STZ). In this study, we prepared an H-2 alloantiserum by injecting C57BL/6J mice (H-2<sup>b</sup>) with spleen lymphocytes from C57BL/KsJ (H-2(d)) mice. The alloantiserum given on five consecutive days, 5 h before each injection of STZ, did not prevent the initial β-cytotoxic effect of STZ detected by perfusion of the pancreas and subsequent morphometric analysis of in situ dithizone-perfused pancreas. However, 12 days after the first injection of STZ, total insulin release in response to D-glucose, total pancreatic insulin, and pancreatic glucagon was greater in the alloantiserum-treated mice compared with controls receiving normal mouse serum. It is concluded that an H-2 alloantiserum may protect the function and amounts of β-cells remaining after the initial five low-dose STZ injections.</p>}}, author = {{Bonnevie-Nielsen, V. and Lernmark, A.}}, issn = {{0012-1797}}, language = {{eng}}, month = {{01}}, number = {{5}}, pages = {{570--573}}, publisher = {{American Diabetes Association Inc.}}, series = {{Diabetes}}, title = {{An H-2 alloantiserum preserves β-cell function in mice made diabetic by low-dose streptozotocin}}, volume = {{35}}, year = {{1986}}, }