High diagnostic accuracy for idiopathic Addison’s disease with a sensitive radiobinding assay for autoantibodies against recombinant human 21-hydroxylase
Falorni, Alberto ; Nikoshkov, Andrej ; Laureti, Stefano ; Grenb�ck, Eva ; Hulting, Anna Lena ; Casucci, Giovanni ; Santeusanio, Fausto ; Brunetti, Paolo ; Luthman, Holger LU and Lernmark, Ǻke LU
(1995)
In Journal of Clinical Endocrinology and Metabolism
80(9).
p.2752-2755
- Abstract
Autoantibodies against 21-hydroxylase (P450c21) are common in idiopathic autoimmune Addison’s disease. In the present work, we have developed a sensitive radiobinding assay using in vitro translated recombinant human 35S-P450c21. Levels of P450c21 antibodies (P450c21-Ab) were expressed as a relative index (P450c21 index) using a P450c21-Ab positive Addisonian serum and two antibody-negative healthy sera as positive and negative standards in healthy individuals. The upper level of normal was the mean + 3 SD. Positivity for P450c21-Ab was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of immunoprecipitated 35S-P450c21. In 38 Addisonian patients, P450c21-Ab were found in... (More)
Autoantibodies against 21-hydroxylase (P450c21) are common in idiopathic autoimmune Addison’s disease. In the present work, we have developed a sensitive radiobinding assay using in vitro translated recombinant human 35S-P450c21. Levels of P450c21 antibodies (P450c21-Ab) were expressed as a relative index (P450c21 index) using a P450c21-Ab positive Addisonian serum and two antibody-negative healthy sera as positive and negative standards in healthy individuals. The upper level of normal was the mean + 3 SD. Positivity for P450c21-Ab was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of immunoprecipitated 35S-P450c21. In 38 Addisonian patients, P450c21-Ab were found in 24/28 (86%) idiopathic, 0/5 post-tuberculosis, 0/3 adrenoleukodystrophy, and 0/2 post-adrenalectomy sera. Among healthy individuals, 1/70 (1.4%) were positive. The P450c21 index, as an estimate of P450c21-Ab levels, correlated inversely with the duration of idiopathic Addison’s disease (r = -0.527; P = 0.007): 16/16 (100%) positive in patients with less than 20 yr and 8/12 (67%) positive in patients with more than 20 yr disease duration. The availability of this simple and sensitive radiobinding assay to evaluate levels of P450c21-Ab will permit large clinical studies as well as screening subjects at risk. In addition, the general population can now be screened to evaluate the predictive value of P450c21-Ab for Addison’s disease.
(Less)
- author
- Falorni, Alberto
; Nikoshkov, Andrej
; Laureti, Stefano
; Grenb�ck, Eva
; Hulting, Anna Lena
; Casucci, Giovanni
; Santeusanio, Fausto
; Brunetti, Paolo
; Luthman, Holger
LU
and Lernmark, Ǻke
LU
- publishing date
- 1995-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Clinical Endocrinology and Metabolism
- volume
- 80
- issue
- 9
- pages
- 4 pages
- publisher
- Oxford University Press
- external identifiers
-
- pmid:7673419
- scopus:0029116295
- ISSN
- 0021-972X
- DOI
- 10.1210/jcem.80.9.7673419
- language
- English
- LU publication?
- no
- id
- 4ec16a22-3331-410a-b9d9-014f20e7edf5
- date added to LUP
- 2019-12-12 23:19:09
- date last changed
- 2024-03-13 08:25:14
@article{4ec16a22-3331-410a-b9d9-014f20e7edf5,
abstract = {{<p>Autoantibodies against 21-hydroxylase (P450c21) are common in idiopathic autoimmune Addison’s disease. In the present work, we have developed a sensitive radiobinding assay using in vitro translated recombinant human <sup>35</sup>S-P450c21. Levels of P450c21 antibodies (P450c21-Ab) were expressed as a relative index (P450c21 index) using a P450c21-Ab positive Addisonian serum and two antibody-negative healthy sera as positive and negative standards in healthy individuals. The upper level of normal was the mean + 3 SD. Positivity for P450c21-Ab was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of immunoprecipitated <sup>35</sup>S-P450c21. In 38 Addisonian patients, P450c21-Ab were found in 24/28 (86%) idiopathic, 0/5 post-tuberculosis, 0/3 adrenoleukodystrophy, and 0/2 post-adrenalectomy sera. Among healthy individuals, 1/70 (1.4%) were positive. The P450c21 index, as an estimate of P450c21-Ab levels, correlated inversely with the duration of idiopathic Addison’s disease (r = -0.527; P = 0.007): 16/16 (100%) positive in patients with less than 20 yr and 8/12 (67%) positive in patients with more than 20 yr disease duration. The availability of this simple and sensitive radiobinding assay to evaluate levels of P450c21-Ab will permit large clinical studies as well as screening subjects at risk. In addition, the general population can now be screened to evaluate the predictive value of P450c21-Ab for Addison’s disease.</p>}},
author = {{Falorni, Alberto and Nikoshkov, Andrej and Laureti, Stefano and Grenb�ck, Eva and Hulting, Anna Lena and Casucci, Giovanni and Santeusanio, Fausto and Brunetti, Paolo and Luthman, Holger and Lernmark, Ǻke}},
issn = {{0021-972X}},
language = {{eng}},
month = {{01}},
number = {{9}},
pages = {{2752--2755}},
publisher = {{Oxford University Press}},
series = {{Journal of Clinical Endocrinology and Metabolism}},
title = {{High diagnostic accuracy for idiopathic Addison’s disease with a sensitive radiobinding assay for autoantibodies against recombinant human 21-hydroxylase}},
url = {{http://dx.doi.org/10.1210/jcem.80.9.7673419}},
doi = {{10.1210/jcem.80.9.7673419}},
volume = {{80}},
year = {{1995}},
}