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Structure, Evolution, and Expression of Proteins in the Male Reproductive Tract. Studies on Semenogelins, Beta-Microseminoprotein, and PC3-Secreted Microprotein.

Valtonen-André, Camilla LU (2007)
Abstract
Human semen has several important constituents, including the prostate secreted proteins beta-microseminoprotein (MSP), prostate-specific antigen (PSA) and prostatic acid phosphatase (PAP), and the seminal-vesicle-derived coagulum proteins semenogelin I (SEMG1) and semenogelin II (SEMG2). The research underlying this thesis explored the evolution, expression and structure of seminal plasma proteins to provide further knowledge in the area of reproduction, particularly regarding MSP and the semenogelins. Several of the studies focused on two species of New World monkeys, that represent evolutionary intermediates between the rodents and humans, and work was also done to characterize a human MSP homologue, and obtain physiological data on... (More)
Human semen has several important constituents, including the prostate secreted proteins beta-microseminoprotein (MSP), prostate-specific antigen (PSA) and prostatic acid phosphatase (PAP), and the seminal-vesicle-derived coagulum proteins semenogelin I (SEMG1) and semenogelin II (SEMG2). The research underlying this thesis explored the evolution, expression and structure of seminal plasma proteins to provide further knowledge in the area of reproduction, particularly regarding MSP and the semenogelins. Several of the studies focused on two species of New World monkeys, that represent evolutionary intermediates between the rodents and humans, and work was also done to characterize a human MSP homologue, and obtain physiological data on human MSP.



The results show that the cotton-top tamarin carries five genes for MSP, one of which exhibits transcriptional activity, and two are nonfunctional pseudogenes. Moreover, the closely related common marmoset has several MSP genes and at least three different forms of MSP in the ejaculate, which also contains semenogelins, but not PAP and PSA. In the marmoset, the genes for both SEMG1 and SEMG2 are transcribed in the seminal vesicles, and they are very similar due to gene conversion. Marmoset SEMG1 has two allelic variants, which differ by one 60-amino-acid repeat.



The studies also revealed the existence of a previously unknown and highly conserved human gene with distant homology to the MSP gene. The protein encoded by this gene is designated PC3-secreted microprotein (PSMP), because it is expressed and secreted by the prostate cancer cell line PC-3, and it can also be detected in benign and malignant prostate tissues.



An immunoassay for MSP was developed and used to measure the protein in serum and seminal plasma from young men. Correlations were found between the levels of MSP in seminal plasma and serum, and also between the concentrations of MSP, zinc and PSA in seminal plasma. Accordingly, we suggest that MSP in serum and semen can serve as a valid marker of prostate secretion. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Professor Eggertsen, Gösta, Department of Laboratory Medicine, Division of Clinical Chemistry, Karolinska Universitetssjukhuset,
organization
publishing date
type
Thesis
publication status
published
subject
keywords
PC3-secreted microprotein, Clinical chemistry, Klinisk kemi, Semenogelin, Beta-microseminoprotein
pages
156 pages
publisher
Department of Laboratory Medicine, Lund University
defense location
Jubileums aulan, Medicinskt Forskningscentrum, ingång 59, Universitetssjukhuset MAS, Malmö
defense date
2007-05-10 09:00:00
ISBN
978-91-85559-44-2
language
English
LU publication?
yes
additional info
Marjaana Mäkinen, Camilla Valtonen-André and Åke Lundwall. 1999. New world, but not old world, monkeys carry several genes encoding beta-microseminoprotein Eur J Biochem, vol 264 pp 407-414.
Camilla Valtonen-André, Yvonne Olsson, Penelope L Nayudu and Åke Lundwall. 2005. Ejaculates from the common marmoset (Callithrix jacchus) contain semenogelin and beta-microseminoprotein but not prostate-specific antigen. Mol Reprod Dev, vol 71 pp 247-255.
Camilla Valtonen-André, A Yvonne Olsson, Morgan Kullberg, Penelope L Nayudu and Åke Lundwall. 2007. The common marmoset (Callithrix jacchus) has two very similar semenogelin genes as the result of gene conversion. Biol Reprod, vol 76 pp 604-610.
Camilla Valtonen-André, Anders Bjartell, Rebecka Hellsten, Hans Lilja, Pirkko Härkönen and Åke Lundwall. 2007. A highly conserved protein secreted by the prostate cancer cell line PC-3 is expressed in benign and malignant prostate tissue. Biol Chem, vol 288 pp 289-295.
Camilla Valtonen-André, Charlotta Sävblom, Per Fernlund, Hans Lilja, Aleksander Giwercman and Åke Lundwall. . Beta-Microseminoprotein in serum correlates with the levels in seminal plasma of young, healthy males. (manuscript)
Camilla Valtonen-André and Åke Lundwall. . The cotton-top tamarin (Saguinus oedipus) has five beta-microseminoprotein genes, two of which are pseudogenes. (manuscript)
id
6e30ee3e-7716-4a47-bd95-51e2e66175e3 (old id 548461)
date added to LUP
2016-04-01 16:09:44
date last changed
2018-11-21 20:39:13
@phdthesis{6e30ee3e-7716-4a47-bd95-51e2e66175e3,
  abstract     = {{Human semen has several important constituents, including the prostate secreted proteins beta-microseminoprotein (MSP), prostate-specific antigen (PSA) and prostatic acid phosphatase (PAP), and the seminal-vesicle-derived coagulum proteins semenogelin I (SEMG1) and semenogelin II (SEMG2). The research underlying this thesis explored the evolution, expression and structure of seminal plasma proteins to provide further knowledge in the area of reproduction, particularly regarding MSP and the semenogelins. Several of the studies focused on two species of New World monkeys, that represent evolutionary intermediates between the rodents and humans, and work was also done to characterize a human MSP homologue, and obtain physiological data on human MSP.<br/><br>
<br/><br>
The results show that the cotton-top tamarin carries five genes for MSP, one of which exhibits transcriptional activity, and two are nonfunctional pseudogenes. Moreover, the closely related common marmoset has several MSP genes and at least three different forms of MSP in the ejaculate, which also contains semenogelins, but not PAP and PSA. In the marmoset, the genes for both SEMG1 and SEMG2 are transcribed in the seminal vesicles, and they are very similar due to gene conversion. Marmoset SEMG1 has two allelic variants, which differ by one 60-amino-acid repeat.<br/><br>
<br/><br>
The studies also revealed the existence of a previously unknown and highly conserved human gene with distant homology to the MSP gene. The protein encoded by this gene is designated PC3-secreted microprotein (PSMP), because it is expressed and secreted by the prostate cancer cell line PC-3, and it can also be detected in benign and malignant prostate tissues.<br/><br>
<br/><br>
An immunoassay for MSP was developed and used to measure the protein in serum and seminal plasma from young men. Correlations were found between the levels of MSP in seminal plasma and serum, and also between the concentrations of MSP, zinc and PSA in seminal plasma. Accordingly, we suggest that MSP in serum and semen can serve as a valid marker of prostate secretion.}},
  author       = {{Valtonen-André, Camilla}},
  isbn         = {{978-91-85559-44-2}},
  keywords     = {{PC3-secreted microprotein; Clinical chemistry; Klinisk kemi; Semenogelin; Beta-microseminoprotein}},
  language     = {{eng}},
  publisher    = {{Department of Laboratory Medicine, Lund University}},
  school       = {{Lund University}},
  title        = {{Structure, Evolution, and Expression of Proteins in the Male Reproductive Tract. Studies on Semenogelins, Beta-Microseminoprotein, and PC3-Secreted Microprotein.}},
  year         = {{2007}},
}