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High efficient ethanol production from corn stover by modified mild alkaline pretreatment

Molaverdi, Maryam LU ; Karimi, Keikhosro ; Mirmohamadsadeghi, Safoora and Galbe, Mats LU (2021) In Renewable Energy 170. p.714-723
Abstract

Sodium carbonate pretreatment, an environmentally-friendly and efficient pretreatment, was used to improve solid-state ethanol production from corn stover (CS). To further enhance ethanol yield from the pretreated CS, especially at low enzyme loadings, a combined pretreatment, including steam and sodium carbonate pretreatments, was developed. The removal/modification of lignin by sodium carbonate pretreatment (at 100 °C for 3 h) helped to obtain high ethanol titer at high solids and low enzymes loadings. The maximum ethanol concentration was about 44 g/L, obtained after 72 h simultaneous saccharification and fermentation (SSF) of CS pretreated with sodium carbonate without mixing and prehydrolysis at 30% solid and 15 FPU/g enzyme... (More)

Sodium carbonate pretreatment, an environmentally-friendly and efficient pretreatment, was used to improve solid-state ethanol production from corn stover (CS). To further enhance ethanol yield from the pretreated CS, especially at low enzyme loadings, a combined pretreatment, including steam and sodium carbonate pretreatments, was developed. The removal/modification of lignin by sodium carbonate pretreatment (at 100 °C for 3 h) helped to obtain high ethanol titer at high solids and low enzymes loadings. The maximum ethanol concentration was about 44 g/L, obtained after 72 h simultaneous saccharification and fermentation (SSF) of CS pretreated with sodium carbonate without mixing and prehydrolysis at 30% solid and 15 FPU/g enzyme loadings. Applying prehydrolysis and increasing the SSF time to 120 h improved ethanol concentration to 59 and 67 g/L, respectively. Steam pretreatment at 190 °C for 10 min before sodium carbonate pretreatment led to 76% increase in ethanol concentration. At the enzyme loading of only 5 FPU/g substrate, the modified pretreatment increased the ethanol concentration from 24 g/L to 41 g/L, compared with that of sodium carbonate pretreatment. The results showed that hemicellulose removal and delignification by steam and sodium carbonate pretreatment, respectively, considerably improved ethanol concentration at high solids and low enzyme loadings.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
High concentration ethanol, High solids loading, Simultaneous hydrolysis and fermentation, Sodium carbonate pretreatment, Two-step pretreatment
in
Renewable Energy
volume
170
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:85100805506
ISSN
0960-1481
DOI
10.1016/j.renene.2021.02.002
language
English
LU publication?
yes
id
55bd12f9-34f7-4f2d-867b-256d2c5b8e8a
date added to LUP
2021-03-01 08:42:51
date last changed
2023-12-19 16:33:12
@article{55bd12f9-34f7-4f2d-867b-256d2c5b8e8a,
  abstract     = {{<p>Sodium carbonate pretreatment, an environmentally-friendly and efficient pretreatment, was used to improve solid-state ethanol production from corn stover (CS). To further enhance ethanol yield from the pretreated CS, especially at low enzyme loadings, a combined pretreatment, including steam and sodium carbonate pretreatments, was developed. The removal/modification of lignin by sodium carbonate pretreatment (at 100 °C for 3 h) helped to obtain high ethanol titer at high solids and low enzymes loadings. The maximum ethanol concentration was about 44 g/L, obtained after 72 h simultaneous saccharification and fermentation (SSF) of CS pretreated with sodium carbonate without mixing and prehydrolysis at 30% solid and 15 FPU/g enzyme loadings. Applying prehydrolysis and increasing the SSF time to 120 h improved ethanol concentration to 59 and 67 g/L, respectively. Steam pretreatment at 190 °C for 10 min before sodium carbonate pretreatment led to 76% increase in ethanol concentration. At the enzyme loading of only 5 FPU/g substrate, the modified pretreatment increased the ethanol concentration from 24 g/L to 41 g/L, compared with that of sodium carbonate pretreatment. The results showed that hemicellulose removal and delignification by steam and sodium carbonate pretreatment, respectively, considerably improved ethanol concentration at high solids and low enzyme loadings.</p>}},
  author       = {{Molaverdi, Maryam and Karimi, Keikhosro and Mirmohamadsadeghi, Safoora and Galbe, Mats}},
  issn         = {{0960-1481}},
  keywords     = {{High concentration ethanol; High solids loading; Simultaneous hydrolysis and fermentation; Sodium carbonate pretreatment; Two-step pretreatment}},
  language     = {{eng}},
  pages        = {{714--723}},
  publisher    = {{Elsevier}},
  series       = {{Renewable Energy}},
  title        = {{High efficient ethanol production from corn stover by modified mild alkaline pretreatment}},
  url          = {{http://dx.doi.org/10.1016/j.renene.2021.02.002}},
  doi          = {{10.1016/j.renene.2021.02.002}},
  volume       = {{170}},
  year         = {{2021}},
}