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Phylogeny of Holocacista capensis (Lepidoptera: Heliozelidae) from Vineyards and Natural Forests in South Africa Inferred from Mitochondrial and Nuclear Genes

Steyn, L. A.I. ; Karsten, M. ; Malan, A. P. ; Wang, H. L. LU and Addison, P. (2023) In South African Journal of Enology and Viticulture 44(1). p.64-74
Abstract

In South Africa, the family Heliozelidae in the order Lepidoptera is restricted to four known species. The grapevine leaf miner, Holocacista capensis, feeds between the epidermal layers of a grapevine leaf, predominantly along the leaf margin. A final instar larva will descend from the blotch mine/gallery to attach its cocoon casing (constructed from the epidermal layers of the mined gallery) to any object below the infested leaf. Five monophyletic clades and a polyphyletic group have been identified within the Heliozelidae, using a mitochondrially encoded gene cytochrome c oxidase I (COI) and a nuclear gene, histone 3 (H3). An exploratory study of the genetic diversity within H. capensis populations was conducted using these genes. The... (More)

In South Africa, the family Heliozelidae in the order Lepidoptera is restricted to four known species. The grapevine leaf miner, Holocacista capensis, feeds between the epidermal layers of a grapevine leaf, predominantly along the leaf margin. A final instar larva will descend from the blotch mine/gallery to attach its cocoon casing (constructed from the epidermal layers of the mined gallery) to any object below the infested leaf. Five monophyletic clades and a polyphyletic group have been identified within the Heliozelidae, using a mitochondrially encoded gene cytochrome c oxidase I (COI) and a nuclear gene, histone 3 (H3). An exploratory study of the genetic diversity within H. capensis populations was conducted using these genes. The phylogenetic analyses of COI indicate that H. capensis that are currently being collected from South Africa fall within three clades/haplotypes, of which one is well supported and contains only one species from Gauteng, and one has three specimens from two different areas in the Western Cape province, while 80% belong to haplotype 1 (H1). The current study can be used as a starting point for future DNA-based studies aimed at gaining insight into possible patterns of diversity in H. capensis to confirm switching from native to commercial grapevine hosts. However, more samples need to be collected from different areas in South Africa.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cocoon casings, COI, grapevine leaf miner, H3, phylogenetic study
in
South African Journal of Enology and Viticulture
volume
44
issue
1
pages
11 pages
publisher
South African Society for Enology and Viticulture
external identifiers
  • scopus:85165043254
ISSN
0253-939X
DOI
10.21548/44-1-5945
language
English
LU publication?
yes
id
56148468-5503-4eee-82f8-d1641e5070fa
date added to LUP
2023-10-03 14:09:46
date last changed
2024-05-17 05:25:05
@article{56148468-5503-4eee-82f8-d1641e5070fa,
  abstract     = {{<p>In South Africa, the family Heliozelidae in the order Lepidoptera is restricted to four known species. The grapevine leaf miner, Holocacista capensis, feeds between the epidermal layers of a grapevine leaf, predominantly along the leaf margin. A final instar larva will descend from the blotch mine/gallery to attach its cocoon casing (constructed from the epidermal layers of the mined gallery) to any object below the infested leaf. Five monophyletic clades and a polyphyletic group have been identified within the Heliozelidae, using a mitochondrially encoded gene cytochrome c oxidase I (COI) and a nuclear gene, histone 3 (H3). An exploratory study of the genetic diversity within H. capensis populations was conducted using these genes. The phylogenetic analyses of COI indicate that H. capensis that are currently being collected from South Africa fall within three clades/haplotypes, of which one is well supported and contains only one species from Gauteng, and one has three specimens from two different areas in the Western Cape province, while 80% belong to haplotype 1 (H1). The current study can be used as a starting point for future DNA-based studies aimed at gaining insight into possible patterns of diversity in H. capensis to confirm switching from native to commercial grapevine hosts. However, more samples need to be collected from different areas in South Africa.</p>}},
  author       = {{Steyn, L. A.I. and Karsten, M. and Malan, A. P. and Wang, H. L. and Addison, P.}},
  issn         = {{0253-939X}},
  keywords     = {{cocoon casings; COI; grapevine leaf miner; H3; phylogenetic study}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{64--74}},
  publisher    = {{South African Society for Enology and Viticulture}},
  series       = {{South African Journal of Enology and Viticulture}},
  title        = {{Phylogeny of Holocacista capensis (Lepidoptera: Heliozelidae) from Vineyards and Natural Forests in South Africa Inferred from Mitochondrial and Nuclear Genes}},
  url          = {{http://dx.doi.org/10.21548/44-1-5945}},
  doi          = {{10.21548/44-1-5945}},
  volume       = {{44}},
  year         = {{2023}},
}