Flow cytometry immunophenotyping in integrated diagnostics of patients with newly diagnosed cytopenia : One tube 10-color 14-antibody screening panel and 3-tube extensive panel for detection of MDS-related features
(2015) In International Journal of Laboratory Hematology 37(S1). p.133-143- Abstract
Acute leukemia, myelodysplastic syndromes (MDS), myeloproliferative neoplasms and lymphomas are the most prevalent diagnoses in adults presenting with new onset cytopenia. Here, we describe two 10-color panels of surface markers (screening and comprehensive panel) applied at the Flow Cytometry Laboratory, University Health Network, Toronto, ON, Canada. A 10-color flow cytometry is applied using the stain-lyse-wash sample preparation method. In patients with <10% blasts and no clear involvement by hematological malignancy based on cytomorphological evaluation of bone marrow (BM) smear, the recently published one-tube 10-color 14-antibody screening panel is applied. This panel allows detection of major B- and T-cell abnormalities,... (More)
Acute leukemia, myelodysplastic syndromes (MDS), myeloproliferative neoplasms and lymphomas are the most prevalent diagnoses in adults presenting with new onset cytopenia. Here, we describe two 10-color panels of surface markers (screening and comprehensive panel) applied at the Flow Cytometry Laboratory, University Health Network, Toronto, ON, Canada. A 10-color flow cytometry is applied using the stain-lyse-wash sample preparation method. In patients with <10% blasts and no clear involvement by hematological malignancy based on cytomorphological evaluation of bone marrow (BM) smear, the recently published one-tube 10-color 14-antibody screening panel is applied. This panel allows detection of major B- and T-cell abnormalities, enumeration of cells in blast region (CD45 dim), and gives insight into myeloid BM compartment, including calculation of four-parameter score for MDS-related abnormalities. In patients who present with ≥10 - <20% blasts in blood or BM smears, a comprehensive three-tube panel of surface markers is used up front. The analysis is focused on the detection of abnormal antigen expression patterns not seen in normal/reactive BM, according to the guidelines developed by International/European LeukemiaNet Working Group for Flow Cytometry in MDS. In patients with ≥20% blasts, an additional tube is added to allow the detection of cytoplasmic markers necessary to diagnose mixed phenotype acute leukemia.
(Less)
- author
- Porwit, A. LU and Rajab, A.
- publishing date
- 2015-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Flow cytometry, Leukemia, Lymphoma, Myelodysplastic syndromes
- in
- International Journal of Laboratory Hematology
- volume
- 37
- issue
- S1
- pages
- 11 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:25976971
- scopus:84929297827
- ISSN
- 1751-5521
- DOI
- 10.1111/ijlh.12368
- language
- English
- LU publication?
- no
- id
- 5735b745-aed4-4523-8039-4531314a348c
- date added to LUP
- 2019-05-22 09:44:52
- date last changed
- 2024-10-02 00:55:50
@article{5735b745-aed4-4523-8039-4531314a348c, abstract = {{<p>Acute leukemia, myelodysplastic syndromes (MDS), myeloproliferative neoplasms and lymphomas are the most prevalent diagnoses in adults presenting with new onset cytopenia. Here, we describe two 10-color panels of surface markers (screening and comprehensive panel) applied at the Flow Cytometry Laboratory, University Health Network, Toronto, ON, Canada. A 10-color flow cytometry is applied using the stain-lyse-wash sample preparation method. In patients with <10% blasts and no clear involvement by hematological malignancy based on cytomorphological evaluation of bone marrow (BM) smear, the recently published one-tube 10-color 14-antibody screening panel is applied. This panel allows detection of major B- and T-cell abnormalities, enumeration of cells in blast region (CD45 dim), and gives insight into myeloid BM compartment, including calculation of four-parameter score for MDS-related abnormalities. In patients who present with ≥10 - <20% blasts in blood or BM smears, a comprehensive three-tube panel of surface markers is used up front. The analysis is focused on the detection of abnormal antigen expression patterns not seen in normal/reactive BM, according to the guidelines developed by International/European LeukemiaNet Working Group for Flow Cytometry in MDS. In patients with ≥20% blasts, an additional tube is added to allow the detection of cytoplasmic markers necessary to diagnose mixed phenotype acute leukemia.</p>}}, author = {{Porwit, A. and Rajab, A.}}, issn = {{1751-5521}}, keywords = {{Flow cytometry; Leukemia; Lymphoma; Myelodysplastic syndromes}}, language = {{eng}}, month = {{01}}, number = {{S1}}, pages = {{133--143}}, publisher = {{Wiley-Blackwell}}, series = {{International Journal of Laboratory Hematology}}, title = {{Flow cytometry immunophenotyping in integrated diagnostics of patients with newly diagnosed cytopenia : One tube 10-color 14-antibody screening panel and 3-tube extensive panel for detection of MDS-related features}}, url = {{http://dx.doi.org/10.1111/ijlh.12368}}, doi = {{10.1111/ijlh.12368}}, volume = {{37}}, year = {{2015}}, }