The effects of embryonic bodies on differentiation of mouse embryonic stem cells to neuronal cells in vitro
(2006) In Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi 23(3). p.620-624- Abstract
This study was directed at assessing the effects of embryonic bodies on differentiation of the embryonic stem cells. The embryonic bodies of different days gained by means of hanging, suspending and hung-suspended were processed by all-trans retinotic acids for 4 days. Then they were detected by immunocytochemistry methods and were measured to detect the fluorescence intensity changes of calcium after Glu stimulation. During this period, we observed the differentiation process and compared the differentiation ratio of embryonic stem cells processed by means of hanging, suspending for several days. It was found that the differentiation ratio of EBs obtained by suspending for 3 days, or by hanging for 3 days and suspending for 1 day, is... (More)
This study was directed at assessing the effects of embryonic bodies on differentiation of the embryonic stem cells. The embryonic bodies of different days gained by means of hanging, suspending and hung-suspended were processed by all-trans retinotic acids for 4 days. Then they were detected by immunocytochemistry methods and were measured to detect the fluorescence intensity changes of calcium after Glu stimulation. During this period, we observed the differentiation process and compared the differentiation ratio of embryonic stem cells processed by means of hanging, suspending for several days. It was found that the differentiation ratio of EBs obtained by suspending for 3 days, or by hanging for 3 days and suspending for 1 day, is relatively higher, which can help us find out the intrinsic differentiation mechanism of embryonic stem cells.
(Less)
- author
- Zhang, Shufang ; Zhang, Enming LU and Zheng, Xiaoxiang
- publishing date
- 2006-06
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
- volume
- 23
- issue
- 3
- pages
- 620 - 624
- publisher
- Sichuan Society for Biomedical Engineering
- external identifiers
-
- pmid:16856402
- scopus:39049183758
- ISSN
- 1001-5515
- language
- English
- LU publication?
- no
- id
- 592bade0-d4d3-4124-ae1a-086c088856c9
- date added to LUP
- 2018-11-14 14:40:13
- date last changed
- 2024-01-15 06:50:05
@article{592bade0-d4d3-4124-ae1a-086c088856c9,
abstract = {{<p>This study was directed at assessing the effects of embryonic bodies on differentiation of the embryonic stem cells. The embryonic bodies of different days gained by means of hanging, suspending and hung-suspended were processed by all-trans retinotic acids for 4 days. Then they were detected by immunocytochemistry methods and were measured to detect the fluorescence intensity changes of calcium after Glu stimulation. During this period, we observed the differentiation process and compared the differentiation ratio of embryonic stem cells processed by means of hanging, suspending for several days. It was found that the differentiation ratio of EBs obtained by suspending for 3 days, or by hanging for 3 days and suspending for 1 day, is relatively higher, which can help us find out the intrinsic differentiation mechanism of embryonic stem cells.</p>}},
author = {{Zhang, Shufang and Zhang, Enming and Zheng, Xiaoxiang}},
issn = {{1001-5515}},
language = {{eng}},
number = {{3}},
pages = {{620--624}},
publisher = {{Sichuan Society for Biomedical Engineering}},
series = {{Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi}},
title = {{The effects of embryonic bodies on differentiation of mouse embryonic stem cells to neuronal cells in vitro}},
volume = {{23}},
year = {{2006}},
}