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Creatine kinase B-driven energy transfer in the brain is important for habituation and spatial learning behaviour, mossy fibre field size and determination of seizure susceptibility

Jost, Carolina R ; Van der Zee, Catharina E. E. M. ; In 't Zandt, H.J.A. LU orcid ; Oerlemans, Frank ; Verheij, Michel ; Streijger, Femke ; Fransen, Jack ; Heerschap, Arend ; Cools, Alexander R and Wieringa, Bé (2002) In European Journal of Neuroscience 15(10). p.706-1692
Abstract

Creatine kinases are important in maintaining cellular-energy homeostasis, and neuroprotective effects have been attributed to the administration of creatine and creatine-like compounds. Herein we examine whether ablation of the cytosolic brain-type creatine kinase (B-CK) in mice has detrimental effects on brain development, physiological integrity or task performance. Mice deficient in B-CK (B-CK-/-) showed no gross abnormalities in brain anatomy or mitochondrial ultrastructure, but had a larger intra- and infrapyramidal mossy fibre area. Nuclear magnetic resonance spectroscopy revealed that adenosine triphosphate (ATP) and phosphocreatine (PCr) levels were unaffected, but demonstrated an apparent reduction of the PCr left arrow over... (More)

Creatine kinases are important in maintaining cellular-energy homeostasis, and neuroprotective effects have been attributed to the administration of creatine and creatine-like compounds. Herein we examine whether ablation of the cytosolic brain-type creatine kinase (B-CK) in mice has detrimental effects on brain development, physiological integrity or task performance. Mice deficient in B-CK (B-CK-/-) showed no gross abnormalities in brain anatomy or mitochondrial ultrastructure, but had a larger intra- and infrapyramidal mossy fibre area. Nuclear magnetic resonance spectroscopy revealed that adenosine triphosphate (ATP) and phosphocreatine (PCr) levels were unaffected, but demonstrated an apparent reduction of the PCr left arrow over right arrow ATP phosphorus exchange capacity in these mice. When assessing behavioural characteristics B-CK-/- animals showed diminished open-field habituation. In the water maze, adult B-CK-/- mice were slower to learn, but acquired the spatial task. This task performance deficit persisted in 24-month-old, aged B-CK-/- mice, on top of the age-related memory decline normally seen in old animals. Finally, a delayed development of pentylenetetrazole-induced seizures (creating a high-energy demand) was observed in B-CK-/- mice. It is suggested that the persistent expression of the mitochondrial isoform ubiquitous mitochondrial CK (UbCKmit) in the creatine/phospho-creatine shuttle provides compensation for the loss of B-CK in the brain. Our studies indicate a role for the creatine-phosphocreatine/CK circuit in the formation or maintenance of hippocampal mossy fibre connections, and processes that involve habituation, spatial learning and seizure susceptibility. However, for fuelling of basic physiological activities the role of B-CK can be compensated for by other systems in the versatile and robust metabolic-energy network of the brain.

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publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Behavior, Animal, Brain, Convulsants, Creatine Kinase, Creatine Kinase, BB Form, Disease Susceptibility, Energy Transfer, Exploratory Behavior, Habituation, Psychophysiologic, Isoenzymes, Magnetic Resonance Spectroscopy, Male, Maze Learning, Mice, Mice, Inbred C57BL, Mice, Knockout, Mossy Fibers, Hippocampal, Neurons, Pentylenetetrazole, Phosphorus, Seizures, Space Perception, Swimming, Journal Article, Research Support, Non-U.S. Gov't
in
European Journal of Neuroscience
volume
15
issue
10
pages
15 pages
publisher
Wiley-Blackwell
external identifiers
  • pmid:12059977
  • scopus:0036460110
ISSN
0953-816X
DOI
10.1046/j.1460-9568.2002.02001.x
language
English
LU publication?
no
id
596df3d1-ac22-4f5b-b8c8-aa17063bac04
date added to LUP
2017-03-06 17:08:43
date last changed
2024-02-29 10:47:09
@article{596df3d1-ac22-4f5b-b8c8-aa17063bac04,
  abstract     = {{<p>Creatine kinases are important in maintaining cellular-energy homeostasis, and neuroprotective effects have been attributed to the administration of creatine and creatine-like compounds. Herein we examine whether ablation of the cytosolic brain-type creatine kinase (B-CK) in mice has detrimental effects on brain development, physiological integrity or task performance. Mice deficient in B-CK (B-CK-/-) showed no gross abnormalities in brain anatomy or mitochondrial ultrastructure, but had a larger intra- and infrapyramidal mossy fibre area. Nuclear magnetic resonance spectroscopy revealed that adenosine triphosphate (ATP) and phosphocreatine (PCr) levels were unaffected, but demonstrated an apparent reduction of the PCr left arrow over right arrow ATP phosphorus exchange capacity in these mice. When assessing behavioural characteristics B-CK-/- animals showed diminished open-field habituation. In the water maze, adult B-CK-/- mice were slower to learn, but acquired the spatial task. This task performance deficit persisted in 24-month-old, aged B-CK-/- mice, on top of the age-related memory decline normally seen in old animals. Finally, a delayed development of pentylenetetrazole-induced seizures (creating a high-energy demand) was observed in B-CK-/- mice. It is suggested that the persistent expression of the mitochondrial isoform ubiquitous mitochondrial CK (UbCKmit) in the creatine/phospho-creatine shuttle provides compensation for the loss of B-CK in the brain. Our studies indicate a role for the creatine-phosphocreatine/CK circuit in the formation or maintenance of hippocampal mossy fibre connections, and processes that involve habituation, spatial learning and seizure susceptibility. However, for fuelling of basic physiological activities the role of B-CK can be compensated for by other systems in the versatile and robust metabolic-energy network of the brain.</p>}},
  author       = {{Jost, Carolina R and Van der Zee, Catharina E. E. M. and In 't Zandt, H.J.A. and Oerlemans, Frank and Verheij, Michel and Streijger, Femke and Fransen, Jack and Heerschap, Arend and Cools, Alexander R and Wieringa, Bé}},
  issn         = {{0953-816X}},
  keywords     = {{Animals; Behavior, Animal; Brain; Convulsants; Creatine Kinase; Creatine Kinase, BB Form; Disease Susceptibility; Energy Transfer; Exploratory Behavior; Habituation, Psychophysiologic; Isoenzymes; Magnetic Resonance Spectroscopy; Male; Maze Learning; Mice; Mice, Inbred C57BL; Mice, Knockout; Mossy Fibers, Hippocampal; Neurons; Pentylenetetrazole; Phosphorus; Seizures; Space Perception; Swimming; Journal Article; Research Support, Non-U.S. Gov't}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{706--1692}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{European Journal of Neuroscience}},
  title        = {{Creatine kinase B-driven energy transfer in the brain is important for habituation and spatial learning behaviour, mossy fibre field size and determination of seizure susceptibility}},
  url          = {{http://dx.doi.org/10.1046/j.1460-9568.2002.02001.x}},
  doi          = {{10.1046/j.1460-9568.2002.02001.x}},
  volume       = {{15}},
  year         = {{2002}},
}