G-Quadruplexes in Nuclear Biomolecular Condensates
(2023) In Genes 14(5).- Abstract
G-quadruplexes (G4s) have long been implicated in the regulation of chromatin packaging and gene expression. These processes require or are accelerated by the separation of related proteins into liquid condensates on DNA/RNA matrices. While cytoplasmic G4s are acknowledged scaffolds of potentially pathogenic condensates, the possible contribution of G4s to phase transitions in the nucleus has only recently come to light. In this review, we summarize the growing evidence for the G4-dependent assembly of biomolecular condensates at telomeres and transcription initiation sites, as well as nucleoli, speckles, and paraspeckles. The limitations of the underlying assays and the remaining open questions are outlined. We also discuss the... (More)
G-quadruplexes (G4s) have long been implicated in the regulation of chromatin packaging and gene expression. These processes require or are accelerated by the separation of related proteins into liquid condensates on DNA/RNA matrices. While cytoplasmic G4s are acknowledged scaffolds of potentially pathogenic condensates, the possible contribution of G4s to phase transitions in the nucleus has only recently come to light. In this review, we summarize the growing evidence for the G4-dependent assembly of biomolecular condensates at telomeres and transcription initiation sites, as well as nucleoli, speckles, and paraspeckles. The limitations of the underlying assays and the remaining open questions are outlined. We also discuss the molecular basis for the apparent permissive role of G4s in the in vitro condensate assembly based on the interactome data. To highlight the prospects and risks of G4-targeting therapies with respect to the phase transitions, we also touch upon the reported effects of G4-stabilizing small molecules on nuclear biomolecular condensates.
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- author
- Pavlova, Iuliia ; Iudin, Mikhail ; Surdina, Anastasiya LU ; Severov, Vjacheslav and Varizhuk, Anna
- publishing date
- 2023-05-13
- type
- Contribution to journal
- publication status
- published
- keywords
- G-Quadruplexes, Biomolecular Condensates, Cell Nucleus/genetics, RNA/genetics, Proteins
- in
- Genes
- volume
- 14
- issue
- 5
- article number
- 1076
- publisher
- MDPI AG
- external identifiers
-
- scopus:85160376250
- pmid:37239436
- ISSN
- 2073-4425
- DOI
- 10.3390/genes14051076
- language
- English
- LU publication?
- no
- id
- 5a863459-6644-49db-ada8-cf35f72f0fb5
- date added to LUP
- 2025-01-17 15:20:22
- date last changed
- 2025-07-19 19:04:46
@article{5a863459-6644-49db-ada8-cf35f72f0fb5,
abstract = {{<p>G-quadruplexes (G4s) have long been implicated in the regulation of chromatin packaging and gene expression. These processes require or are accelerated by the separation of related proteins into liquid condensates on DNA/RNA matrices. While cytoplasmic G4s are acknowledged scaffolds of potentially pathogenic condensates, the possible contribution of G4s to phase transitions in the nucleus has only recently come to light. In this review, we summarize the growing evidence for the G4-dependent assembly of biomolecular condensates at telomeres and transcription initiation sites, as well as nucleoli, speckles, and paraspeckles. The limitations of the underlying assays and the remaining open questions are outlined. We also discuss the molecular basis for the apparent permissive role of G4s in the in vitro condensate assembly based on the interactome data. To highlight the prospects and risks of G4-targeting therapies with respect to the phase transitions, we also touch upon the reported effects of G4-stabilizing small molecules on nuclear biomolecular condensates.</p>}},
author = {{Pavlova, Iuliia and Iudin, Mikhail and Surdina, Anastasiya and Severov, Vjacheslav and Varizhuk, Anna}},
issn = {{2073-4425}},
keywords = {{G-Quadruplexes; Biomolecular Condensates; Cell Nucleus/genetics; RNA/genetics; Proteins}},
language = {{eng}},
month = {{05}},
number = {{5}},
publisher = {{MDPI AG}},
series = {{Genes}},
title = {{G-Quadruplexes in Nuclear Biomolecular Condensates}},
url = {{http://dx.doi.org/10.3390/genes14051076}},
doi = {{10.3390/genes14051076}},
volume = {{14}},
year = {{2023}},
}