Affinity fibre — a new support for rapid enzyme purification by high-performance liquid affinity chromatography
(1987) In Journal of chromatography. A 388. p.123-134- Abstract
- A new type of support for chromatographic use has been developed. Non-porous quartz fibre, with a mean diameter of 0.5 μm, was silylated with mercaptopropyltrimethoxysilane. Tresyl chloride-activated dextran was covalently coupled to the SH groups on the fibre. Remaining active tresyl groups on the dextran were then coupled with an NAD derivative.
The affinity fibre contained 0.3 μmol NAD derivative per g and awas able to bind 15 mg of lactate dehydrogenase per g of fibre.
The affinity fibre was used for large-scale purification of ox heart lactate dehydrogenase and its performance was compared with other commonly used chromatographic matrices. The operational capacity was found to be 1.0 g of pure lactate dehydrogenase... (More) - A new type of support for chromatographic use has been developed. Non-porous quartz fibre, with a mean diameter of 0.5 μm, was silylated with mercaptopropyltrimethoxysilane. Tresyl chloride-activated dextran was covalently coupled to the SH groups on the fibre. Remaining active tresyl groups on the dextran were then coupled with an NAD derivative.
The affinity fibre contained 0.3 μmol NAD derivative per g and awas able to bind 15 mg of lactate dehydrogenase per g of fibre.
The affinity fibre was used for large-scale purification of ox heart lactate dehydrogenase and its performance was compared with other commonly used chromatographic matrices. The operational capacity was found to be 1.0 g of pure lactate dehydrogenase per hour per 100 g opf fibre material (adsorption followed by salt elution). The affinity fibre was found to be particularly suitable for very rapid processing of large volumes of dilute enzyme solutions. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5aa3450a-3a2d-4427-b0bc-9c1756646ab0
- author
- Wikström, Per and Larsson, Per-Olof LU
- organization
- publishing date
- 1987
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Affinity fibre, sub-micron fibre, Lactate dehydrogenase, Dextran-coated fibre
- in
- Journal of chromatography. A
- volume
- 388
- pages
- 12 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0023646158
- ISSN
- 0021-9673
- DOI
- 10.1016/S0021-9673(01)94473-5
- language
- English
- LU publication?
- yes
- id
- 5aa3450a-3a2d-4427-b0bc-9c1756646ab0
- date added to LUP
- 2024-06-18 14:28:36
- date last changed
- 2024-09-04 09:40:05
@article{5aa3450a-3a2d-4427-b0bc-9c1756646ab0,
abstract = {{A new type of support for chromatographic use has been developed. Non-porous quartz fibre, with a mean diameter of 0.5 μm, was silylated with mercaptopropyltrimethoxysilane. Tresyl chloride-activated dextran was covalently coupled to the SH groups on the fibre. Remaining active tresyl groups on the dextran were then coupled with an NAD derivative.<br/><br/>The affinity fibre contained 0.3 μmol NAD derivative per g and awas able to bind 15 mg of lactate dehydrogenase per g of fibre.<br/><br/>The affinity fibre was used for large-scale purification of ox heart lactate dehydrogenase and its performance was compared with other commonly used chromatographic matrices. The operational capacity was found to be 1.0 g of pure lactate dehydrogenase per hour per 100 g opf fibre material (adsorption followed by salt elution). The affinity fibre was found to be particularly suitable for very rapid processing of large volumes of dilute enzyme solutions.}},
author = {{Wikström, Per and Larsson, Per-Olof}},
issn = {{0021-9673}},
keywords = {{Affinity fibre; sub-micron fibre; Lactate dehydrogenase; Dextran-coated fibre}},
language = {{eng}},
pages = {{123--134}},
publisher = {{Elsevier}},
series = {{Journal of chromatography. A}},
title = {{Affinity fibre — a new support for rapid enzyme purification by high-performance liquid affinity chromatography}},
url = {{http://dx.doi.org/10.1016/S0021-9673(01)94473-5}},
doi = {{10.1016/S0021-9673(01)94473-5}},
volume = {{388}},
year = {{1987}},
}