Multimodal Mass Spectrometry Identifies a Conserved Protective Epitope in S. pyogenes Streptolysin O
(2024) In Analytical Chemistry 96(22). p.9060-9068- Abstract
- An important element of antibody-guided vaccine design is the use of neutralizing or opsonic monoclonal antibodies to define protective epitopes in their native three-dimensional conformation. Here, we demonstrate a multimodal mass spectrometry-based strategy for in-depth characterization of antigen–antibody complexes to enable the identification of protective epitopes using the cytolytic exotoxin Streptolysin O (SLO) from Streptococcus pyogenes as a showcase. We first discovered a monoclonal antibody with an undisclosed sequence capable of neutralizing SLO-mediated cytolysis. The amino acid sequence of both the antibody light and the heavy chain was determined using mass-spectrometry-based de novo sequencing, followed by... (More)
- An important element of antibody-guided vaccine design is the use of neutralizing or opsonic monoclonal antibodies to define protective epitopes in their native three-dimensional conformation. Here, we demonstrate a multimodal mass spectrometry-based strategy for in-depth characterization of antigen–antibody complexes to enable the identification of protective epitopes using the cytolytic exotoxin Streptolysin O (SLO) from Streptococcus pyogenes as a showcase. We first discovered a monoclonal antibody with an undisclosed sequence capable of neutralizing SLO-mediated cytolysis. The amino acid sequence of both the antibody light and the heavy chain was determined using mass-spectrometry-based de novo sequencing, followed by chemical cross-linking mass spectrometry to generate distance constraints between the antibody fragment antigen-binding region and SLO. Subsequent integrative computational modeling revealed a discontinuous epitope located in domain 3 of SLO that was experimentally validated by hydrogen–deuterium exchange mass spectrometry and reverse engineering of the targeted epitope. The results show that the antibody inhibits SLO-mediated cytolysis by binding to a discontinuous epitope in domain 3, likely preventing oligomerization and subsequent secondary structure transitions critical for pore-formation. The epitope is highly conserved across >98% of the characterized S. pyogenes isolates, making it an attractive target for antibody-based therapy and vaccine design against severe streptococcal infections. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5d070d7b-46b1-4bdc-b1a2-62b1a026cd78
- author
- Tang, Di LU ; Gueto-Tettay, Carlos LU ; Hjortswang, Elisabeth LU ; Ströbaek, Joel LU ; Ekström, Simon LU ; Happonen, Lotta LU ; Malmström, Lars LU and Malmström, Johan LU
- organization
-
- Infection Medicine (BMC)
- Infection Medicine Proteomics (research group)
- epIgG (research group)
- SciLifeLab Site@Lund
- Structural Infection Medicine (STRIME) (research group)
- BioMS (research group)
- LTH Profile Area: Engineering Health
- Mass Spectrometry
- SEBRA Sepsis and Bacterial Resistance Alliance (research group)
- publishing date
- 2024-06-04
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 96
- issue
- 22
- pages
- 9060 - 9068
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:38701337
- scopus:85192310157
- ISSN
- 1520-6882
- DOI
- 10.1021/acs.analchem.4c00596
- project
- Proof-of-concept design of multivalent nanoparticle vaccines against antibiotic-resistant bacteria
- Properties of Protective Antibody Responses against Bacterial Pathogens
- language
- English
- LU publication?
- yes
- id
- 5d070d7b-46b1-4bdc-b1a2-62b1a026cd78
- date added to LUP
- 2024-05-06 16:39:46
- date last changed
- 2024-07-12 04:01:28
@article{5d070d7b-46b1-4bdc-b1a2-62b1a026cd78, abstract = {{An important element of antibody-guided vaccine design is the use of neutralizing or opsonic monoclonal antibodies to define protective epitopes in their native three-dimensional conformation. Here, we demonstrate a multimodal mass spectrometry-based strategy for in-depth characterization of antigen–antibody complexes to enable the identification of protective epitopes using the cytolytic exotoxin Streptolysin O (SLO) from <i>Streptococcus pyogenes</i> as a showcase. We first discovered a monoclonal antibody with an undisclosed sequence capable of neutralizing SLO-mediated cytolysis. The amino acid sequence of both the antibody light and the heavy chain was determined using mass-spectrometry-based <i>de novo</i> sequencing, followed by chemical cross-linking mass spectrometry to generate distance constraints between the antibody fragment antigen-binding region and SLO. Subsequent integrative computational modeling revealed a discontinuous epitope located in domain 3 of SLO that was experimentally validated by hydrogen–deuterium exchange mass spectrometry and reverse engineering of the targeted epitope. The results show that the antibody inhibits SLO-mediated cytolysis by binding to a discontinuous epitope in domain 3, likely preventing oligomerization and subsequent secondary structure transitions critical for pore-formation. The epitope is highly conserved across >98% of the characterized <i>S. pyogenes</i> isolates, making it an attractive target for antibody-based therapy and vaccine design against severe streptococcal infections.}}, author = {{Tang, Di and Gueto-Tettay, Carlos and Hjortswang, Elisabeth and Ströbaek, Joel and Ekström, Simon and Happonen, Lotta and Malmström, Lars and Malmström, Johan}}, issn = {{1520-6882}}, language = {{eng}}, month = {{06}}, number = {{22}}, pages = {{9060--9068}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Analytical Chemistry}}, title = {{Multimodal Mass Spectrometry Identifies a Conserved Protective Epitope in <i>S. pyogenes</i> Streptolysin O}}, url = {{http://dx.doi.org/10.1021/acs.analchem.4c00596}}, doi = {{10.1021/acs.analchem.4c00596}}, volume = {{96}}, year = {{2024}}, }