Steroid hydroxylation using immobilized spores of Curvularia lunata germinated in situ
(1980) In European Journal of Applied Microbiology and Biotechnology 10. p.1-9- Abstract
- Spores of Curvularia lunata were immobilized in polyacrylamide granules and in calcium alginate beads (2–3 mm in diam.). Germination of the spores, initiated by the addition of nutrients, resulted in an even distribution of mycelium throughout the beads after 48 h. Such beads were used for the conversion of cortexolone to cortisol by steroid-11β-hydroxylation. In order to improve the steroid transforming ability several parameters were studied. It was found that preparations based on calcium alginate gave the best results.
The possible merits of immobilizing spores rather than vegetative cells, followed by in situ germination are discussed also for other microorganisms and immobilization processes.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5d97e991-51f2-4b91-8f98-a2337bee566f
- author
- Ohlson, Sten ; Flygare, Susanne ; Larsson, Per-Olof LU and Mosbach, Klaus LU
- organization
- publishing date
- 1980
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Immobilized spores, Curvularia lunata, In situ germination
- in
- European Journal of Applied Microbiology and Biotechnology
- volume
- 10
- pages
- 9 pages
- publisher
- Springer
- external identifiers
-
- scopus:0002547527
- ISSN
- 0171-1741
- DOI
- 10.1007/BF00504722
- language
- English
- LU publication?
- yes
- id
- 5d97e991-51f2-4b91-8f98-a2337bee566f
- date added to LUP
- 2024-06-19 09:44:29
- date last changed
- 2024-09-09 10:04:44
@article{5d97e991-51f2-4b91-8f98-a2337bee566f, abstract = {{Spores of <i>Curvularia lunata</i> were immobilized in polyacrylamide granules and in calcium alginate beads (2–3 mm in diam.). Germination of the spores, initiated by the addition of nutrients, resulted in an even distribution of mycelium throughout the beads after 48 h. Such beads were used for the conversion of cortexolone to cortisol by steroid-11<i>β</i>-hydroxylation. In order to improve the steroid transforming ability several parameters were studied. It was found that preparations based on calcium alginate gave the best results.<br/><br/>The possible merits of immobilizing spores rather than vegetative cells, followed by in situ germination are discussed also for other microorganisms and immobilization processes.}}, author = {{Ohlson, Sten and Flygare, Susanne and Larsson, Per-Olof and Mosbach, Klaus}}, issn = {{0171-1741}}, keywords = {{Immobilized spores; Curvularia lunata; In situ germination}}, language = {{eng}}, pages = {{1--9}}, publisher = {{Springer}}, series = {{European Journal of Applied Microbiology and Biotechnology}}, title = {{Steroid hydroxylation using immobilized spores of <i>Curvularia lunata</i> germinated in situ}}, url = {{http://dx.doi.org/10.1007/BF00504722}}, doi = {{10.1007/BF00504722}}, volume = {{10}}, year = {{1980}}, }