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Absorption of kininogen from human plasma by Streptococcus pyogenes is followed by the release of bradykinin

Ben Nasr, Abdelhakim ; Herwald, Heiko LU orcid ; Sjöbring, Ulf LU ; Renné, Thomas ; Müller-Esterl, Werner and Björck, Lars LU (1997) In Biochemical Journal 326(3). p.657-660
Abstract

H-kininogen (high-molecular-mass kininogen, HK) is the precursor of the vasoactive peptide hormone bradykinin (BK). Previous work has demonstrated that HK binds to Streptococcus pyogenes through M-proteins, fibrous surface proteins and important virulence factors of these bacteria. Here we find that M-protein-expressing bacteria absorb HK from human plasma. The HK bound to the bacteria was found to be cleaved, and analysis of the degradation pattern suggested that the cleavage of HK at the bacterial surface is associated with the release of BK. Moreover, addition of activated plasma prekallikrein to bacteria preincubated with human plasma, resulted in BK release. This mechanism, by which a potent vasoactive and proinflammatory peptide... (More)

H-kininogen (high-molecular-mass kininogen, HK) is the precursor of the vasoactive peptide hormone bradykinin (BK). Previous work has demonstrated that HK binds to Streptococcus pyogenes through M-proteins, fibrous surface proteins and important virulence factors of these bacteria. Here we find that M-protein-expressing bacteria absorb HK from human plasma. The HK bound to the bacteria was found to be cleaved, and analysis of the degradation pattern suggested that the cleavage of HK at the bacterial surface is associated with the release of BK. Moreover, addition of activated plasma prekallikrein to bacteria preincubated with human plasma, resulted in BK release. This mechanism, by which a potent vasoactive and proinflammatory peptide is generated at the site of infection, should influence the host-parasite relationship during S. pyogenes infections.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemical Journal
volume
326
issue
3
pages
657 - 660
publisher
Portland Press
external identifiers
  • scopus:0030802005
  • pmid:9307013
ISSN
0264-6021
DOI
10.1042/bj3260657
language
English
LU publication?
yes
id
5ef53a61-f222-482b-83cf-ef29696dce17
date added to LUP
2019-05-27 06:39:38
date last changed
2024-01-30 19:34:42
@article{5ef53a61-f222-482b-83cf-ef29696dce17,
  abstract     = {{<p>H-kininogen (high-molecular-mass kininogen, HK) is the precursor of the vasoactive peptide hormone bradykinin (BK). Previous work has demonstrated that HK binds to Streptococcus pyogenes through M-proteins, fibrous surface proteins and important virulence factors of these bacteria. Here we find that M-protein-expressing bacteria absorb HK from human plasma. The HK bound to the bacteria was found to be cleaved, and analysis of the degradation pattern suggested that the cleavage of HK at the bacterial surface is associated with the release of BK. Moreover, addition of activated plasma prekallikrein to bacteria preincubated with human plasma, resulted in BK release. This mechanism, by which a potent vasoactive and proinflammatory peptide is generated at the site of infection, should influence the host-parasite relationship during S. pyogenes infections.</p>}},
  author       = {{Ben Nasr, Abdelhakim and Herwald, Heiko and Sjöbring, Ulf and Renné, Thomas and Müller-Esterl, Werner and Björck, Lars}},
  issn         = {{0264-6021}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{3}},
  pages        = {{657--660}},
  publisher    = {{Portland Press}},
  series       = {{Biochemical Journal}},
  title        = {{Absorption of kininogen from human plasma by Streptococcus pyogenes is followed by the release of bradykinin}},
  url          = {{http://dx.doi.org/10.1042/bj3260657}},
  doi          = {{10.1042/bj3260657}},
  volume       = {{326}},
  year         = {{1997}},
}