Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells.
(1983) In Proceedings of the National Academy of Sciences of the United States of America 80(12). p.3758-3761- Abstract
Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of... (More)
Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.
(Less)
- author
- Owerbach, D. ; Lernmark, A. LU ; Rask, L. ; Peterson, P. A. ; Platz, P. and Svejgaard, A.
- publishing date
- 1983-01-01
- type
- Contribution to journal
- publication status
- published
- in
- Proceedings of the National Academy of Sciences of the United States of America
- volume
- 80
- issue
- 12
- pages
- 4 pages
- publisher
- National Academy of Sciences
- external identifiers
-
- scopus:0020772532
- pmid:6304735
- ISSN
- 0027-8424
- DOI
- 10.1073/pnas.80.12.3758
- language
- English
- LU publication?
- no
- id
- 5f667838-1000-44a3-b503-79a522887d52
- date added to LUP
- 2019-09-16 12:45:03
- date last changed
- 2024-03-13 08:10:41
@article{5f667838-1000-44a3-b503-79a522887d52, abstract = {{<p>Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.</p>}}, author = {{Owerbach, D. and Lernmark, A. and Rask, L. and Peterson, P. A. and Platz, P. and Svejgaard, A.}}, issn = {{0027-8424}}, language = {{eng}}, month = {{01}}, number = {{12}}, pages = {{3758--3761}}, publisher = {{National Academy of Sciences}}, series = {{Proceedings of the National Academy of Sciences of the United States of America}}, title = {{Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells.}}, url = {{http://dx.doi.org/10.1073/pnas.80.12.3758}}, doi = {{10.1073/pnas.80.12.3758}}, volume = {{80}}, year = {{1983}}, }