Loss of SNAP-25 and rabphilin 3a in sensory-motor cortex in Huntington's disease.
(2007) In Journal of Neurochemistry 103(1). p.115-123- Abstract
- Huntington’s disease (HD) is an autosomal dominant neurodegenerative disorder caused by a CAG-expansion in the gene encoding the protein huntingtin. The disease is characterized by progressive motor disturbances, cognitive defects, dementia, and weight loss. Using western blotting and immunohistochemistry we have assessed the expression levels and patterns of a number of proteins involved in neurotransmitter release in post-mortem frontal cortex samples from 10 HD cases with different disease grades. We report a loss of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein, synaptosome-associated protein 25 (SNAP 25) in HD brains of grades I–IV. Moreover, in brains of grade III and IV we found a... (More)
- Huntington’s disease (HD) is an autosomal dominant neurodegenerative disorder caused by a CAG-expansion in the gene encoding the protein huntingtin. The disease is characterized by progressive motor disturbances, cognitive defects, dementia, and weight loss. Using western blotting and immunohistochemistry we have assessed the expression levels and patterns of a number of proteins involved in neurotransmitter release in post-mortem frontal cortex samples from 10 HD cases with different disease grades. We report a loss of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein, synaptosome-associated protein 25 (SNAP 25) in HD brains of grades I–IV. Moreover, in brains of grade III and IV we found a reduction in rabphilin 3a, a protein involved in vesicle docking and recycling. These losses appear to be specific and not due to a general loss of synapses in the HD cortex. Thus, levels of synaptobrevin II, syntaxin 1, rab3a or synaptophysin are unaltered in the same patient samples. SNAP 25 and rabphilin 3a are crucial for neurotransmitter release. Therefore, we suggest that a deficient pre-synaptic transmitter release may underlie some of the symptoms of HD. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/607693
- author
- Smith, Ruben LU ; Klein, Pontus LU ; Koc-Schmitz, Yeliz ; Waldvogel, Henry J ; Faull, Richard L M ; Brundin, Patrik LU ; Plomann, Markus and Li, Jia-Yi
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Neurochemistry
- volume
- 103
- issue
- 1
- pages
- 115 - 123
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000249949700010
- scopus:34548626196
- ISSN
- 1471-4159
- DOI
- 10.1111/j.1471-4159.2007.04703.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuronal Survival (013212041)
- id
- bcf36e82-5012-48ff-b8f2-c3def8bb5dcf (old id 607693)
- date added to LUP
- 2016-04-01 16:04:45
- date last changed
- 2022-04-15 01:55:02
@article{bcf36e82-5012-48ff-b8f2-c3def8bb5dcf, abstract = {{Huntington’s disease (HD) is an autosomal dominant neurodegenerative disorder caused by a CAG-expansion in the gene encoding the protein huntingtin. The disease is characterized by progressive motor disturbances, cognitive defects, dementia, and weight loss. Using western blotting and immunohistochemistry we have assessed the expression levels and patterns of a number of proteins involved in neurotransmitter release in post-mortem frontal cortex samples from 10 HD cases with different disease grades. We report a loss of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein, synaptosome-associated protein 25 (SNAP 25) in HD brains of grades I–IV. Moreover, in brains of grade III and IV we found a reduction in rabphilin 3a, a protein involved in vesicle docking and recycling. These losses appear to be specific and not due to a general loss of synapses in the HD cortex. Thus, levels of synaptobrevin II, syntaxin 1, rab3a or synaptophysin are unaltered in the same patient samples. SNAP 25 and rabphilin 3a are crucial for neurotransmitter release. Therefore, we suggest that a deficient pre-synaptic transmitter release may underlie some of the symptoms of HD.}}, author = {{Smith, Ruben and Klein, Pontus and Koc-Schmitz, Yeliz and Waldvogel, Henry J and Faull, Richard L M and Brundin, Patrik and Plomann, Markus and Li, Jia-Yi}}, issn = {{1471-4159}}, language = {{eng}}, number = {{1}}, pages = {{115--123}}, publisher = {{Wiley-Blackwell}}, series = {{Journal of Neurochemistry}}, title = {{Loss of SNAP-25 and rabphilin 3a in sensory-motor cortex in Huntington's disease.}}, url = {{https://lup.lub.lu.se/search/files/4560701/626129.pdf}}, doi = {{10.1111/j.1471-4159.2007.04703.x}}, volume = {{103}}, year = {{2007}}, }