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The HEM Proteins : A novel family of tissue-specific transmembrane proteins expressed from invertebrates through mammals with an essential function in oogenesis

Baumgartner, Stefan LU orcid ; Martin, Doris ; Chiquet-Ehrismann, Ruth ; Sutton, Jill ; Desai, Alpana ; Huang, Irene ; Kato, Kikuya and Hromas, Robert (1995) In Journal of Molecular Biology 251(1). p.41-49
Abstract

We report the identification of a new family of proteins, termed the HEM family, which show distinct expression patterns in blood cells and the central nervous system. Through the isolation and characterization of the corresponding brain-specificDrosophila(dhem-2) and rat orthologues (Hem-2), and through the detection of theCaenorhabditis elegansHem-2 orthologue in the database, we show that this family is conserved throughout evolution. HEM proteins show a conserved length ranging from 1118 to 1126 amino acid residues. Moreover, they are at least 35% identical with each other and harbour several conserved membrane-spanning domains, indicative for their location on the cell surface. One of the members, theDrosophilaorthologuedhem-2, was... (More)

We report the identification of a new family of proteins, termed the HEM family, which show distinct expression patterns in blood cells and the central nervous system. Through the isolation and characterization of the corresponding brain-specificDrosophila(dhem-2) and rat orthologues (Hem-2), and through the detection of theCaenorhabditis elegansHem-2 orthologue in the database, we show that this family is conserved throughout evolution. HEM proteins show a conserved length ranging from 1118 to 1126 amino acid residues. Moreover, they are at least 35% identical with each other and harbour several conserved membrane-spanning domains, indicative for their location on the cell surface. One of the members, theDrosophilaorthologuedhem-2, was analysed in detail for its spatial expression pattern during development and for its mutant phenotype.dhem-2is expressed maternally in the oocyte and shows uniform expression during the first half of embryogenesis, but becomes restricted to the brain and the nervous system during late embryogenesis, consistent with the expression of its vertebrate orthologue in the brain. One P-element insertion, located 39 base-pairs downstream from thedhem-2transcription start site, causes female sterility, due to the fact that developmental processes in the oocyte are disturbed. Of the vertebrate HEM family members, the mammalian Hem-1 gene is expressed only in cells of hematopoietic origin, while Hem-2 is preferentially expressed in brain, heart, liver and testis.f1 f1 Correspondence concerningDrosophilaHEM genes should be addressed to S.B. Correspondence concerning the vertebrate HEM genes should be addressed to R.H.

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author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Drosophila, Evolution, HEM family, Maternal lethal, Transmembrane protein
in
Journal of Molecular Biology
volume
251
issue
1
pages
41 - 49
publisher
Elsevier
external identifiers
  • scopus:0029085005
  • pmid:7643388
ISSN
0022-2836
DOI
10.1006/jmbi.1995.0414
language
English
LU publication?
no
id
62010f68-0f5e-450d-bfd4-926152ba1e6c
date added to LUP
2019-05-21 14:07:39
date last changed
2024-01-01 05:55:33
@article{62010f68-0f5e-450d-bfd4-926152ba1e6c,
  abstract     = {{<p>We report the identification of a new family of proteins, termed the HEM family, which show distinct expression patterns in blood cells and the central nervous system. Through the isolation and characterization of the corresponding brain-specificDrosophila(dhem-2) and rat orthologues (Hem-2), and through the detection of theCaenorhabditis elegansHem-2 orthologue in the database, we show that this family is conserved throughout evolution. HEM proteins show a conserved length ranging from 1118 to 1126 amino acid residues. Moreover, they are at least 35% identical with each other and harbour several conserved membrane-spanning domains, indicative for their location on the cell surface. One of the members, theDrosophilaorthologuedhem-2, was analysed in detail for its spatial expression pattern during development and for its mutant phenotype.dhem-2is expressed maternally in the oocyte and shows uniform expression during the first half of embryogenesis, but becomes restricted to the brain and the nervous system during late embryogenesis, consistent with the expression of its vertebrate orthologue in the brain. One P-element insertion, located 39 base-pairs downstream from thedhem-2transcription start site, causes female sterility, due to the fact that developmental processes in the oocyte are disturbed. Of the vertebrate HEM family members, the mammalian Hem-1 gene is expressed only in cells of hematopoietic origin, while Hem-2 is preferentially expressed in brain, heart, liver and testis.<sup>f1</sup> f1 Correspondence concerningDrosophilaHEM genes should be addressed to S.B. Correspondence concerning the vertebrate HEM genes should be addressed to R.H.</p>}},
  author       = {{Baumgartner, Stefan and Martin, Doris and Chiquet-Ehrismann, Ruth and Sutton, Jill and Desai, Alpana and Huang, Irene and Kato, Kikuya and Hromas, Robert}},
  issn         = {{0022-2836}},
  keywords     = {{Drosophila; Evolution; HEM family; Maternal lethal; Transmembrane protein}},
  language     = {{eng}},
  month        = {{08}},
  number       = {{1}},
  pages        = {{41--49}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Molecular Biology}},
  title        = {{The HEM Proteins : A novel family of tissue-specific transmembrane proteins expressed from invertebrates through mammals with an essential function in oogenesis}},
  url          = {{http://dx.doi.org/10.1006/jmbi.1995.0414}},
  doi          = {{10.1006/jmbi.1995.0414}},
  volume       = {{251}},
  year         = {{1995}},
}