Poly(acrylamide-allyl glycidyl ether) cryogel as a novel stationary phase in dye-affinity chromatography
(2007) In Journal of Applied Polymer Science 105(4). p.1808-1816- Abstract
- Poly(acrylamide-allyl glycidyl ether) [poly(AAm-AGE)] cryogel was prepared by bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 mu m size. Cibacron Blue F3GA was immobilized by covalent binding onto poly(AAm-AGE) cryogel via epoxy groups. Poly(AAm-AGE) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium swelling degree of the poly(AAm-AGE) monolithic cryogel was 6.84 g H2O/9 cryogel. Poly(AAm-AGE) cryogel containing 68.9 mu mol Cibacron Blue F3GA/g was used in the adsorption/desorption... (More)
- Poly(acrylamide-allyl glycidyl ether) [poly(AAm-AGE)] cryogel was prepared by bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 mu m size. Cibacron Blue F3GA was immobilized by covalent binding onto poly(AAm-AGE) cryogel via epoxy groups. Poly(AAm-AGE) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium swelling degree of the poly(AAm-AGE) monolithic cryogel was 6.84 g H2O/9 cryogel. Poly(AAm-AGE) cryogel containing 68.9 mu mol Cibacron Blue F3GA/g was used in the adsorption/desorption of human serum albumin (HSA) from aqueous solutions and human plasma. The nonspecific adsorption of HSA was very low (0.2 mg/g). The maximum amount of HSA adsorption from aqueous solution in acetate buffer was 27 mg/g at pH 5.0. Higher HSA adsorption value was obtained from human plasma (up to 74.2 mg/g). Desorption of HSA with a purity of 92% from Cibacron Blue F3GA attached poly(AAm-AGE) cryogel was achieved using 0.110 Tris/HCl buffer containing 0.5M NaCl. It was observed that HSA could be repeatedly adsorbed and desorbed with poly(AAm-AGE) cryogel without significant loss in the adsorption capacity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/647724
- author
- Demiryas, Nazan ; Tuzmen, Nalan ; Galaev, Igor LU ; Piskin, Erhan and Denizli, Adil
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- albumin, protein purification, dye-affinity chromatography, cryogels
- in
- Journal of Applied Polymer Science
- volume
- 105
- issue
- 4
- pages
- 1808 - 1816
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000247576600012
- scopus:34547165579
- ISSN
- 1097-4628
- DOI
- 10.1002/app.26187
- language
- English
- LU publication?
- yes
- id
- c266475c-e1b0-403d-a592-e6d9c6441063 (old id 647724)
- date added to LUP
- 2016-04-01 11:33:43
- date last changed
- 2025-04-04 14:26:02
@article{c266475c-e1b0-403d-a592-e6d9c6441063,
abstract = {{Poly(acrylamide-allyl glycidyl ether) [poly(AAm-AGE)] cryogel was prepared by bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 mu m size. Cibacron Blue F3GA was immobilized by covalent binding onto poly(AAm-AGE) cryogel via epoxy groups. Poly(AAm-AGE) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium swelling degree of the poly(AAm-AGE) monolithic cryogel was 6.84 g H2O/9 cryogel. Poly(AAm-AGE) cryogel containing 68.9 mu mol Cibacron Blue F3GA/g was used in the adsorption/desorption of human serum albumin (HSA) from aqueous solutions and human plasma. The nonspecific adsorption of HSA was very low (0.2 mg/g). The maximum amount of HSA adsorption from aqueous solution in acetate buffer was 27 mg/g at pH 5.0. Higher HSA adsorption value was obtained from human plasma (up to 74.2 mg/g). Desorption of HSA with a purity of 92% from Cibacron Blue F3GA attached poly(AAm-AGE) cryogel was achieved using 0.110 Tris/HCl buffer containing 0.5M NaCl. It was observed that HSA could be repeatedly adsorbed and desorbed with poly(AAm-AGE) cryogel without significant loss in the adsorption capacity.}},
author = {{Demiryas, Nazan and Tuzmen, Nalan and Galaev, Igor and Piskin, Erhan and Denizli, Adil}},
issn = {{1097-4628}},
keywords = {{albumin; protein purification; dye-affinity chromatography; cryogels}},
language = {{eng}},
number = {{4}},
pages = {{1808--1816}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Applied Polymer Science}},
title = {{Poly(acrylamide-allyl glycidyl ether) cryogel as a novel stationary phase in dye-affinity chromatography}},
url = {{http://dx.doi.org/10.1002/app.26187}},
doi = {{10.1002/app.26187}},
volume = {{105}},
year = {{2007}},
}