High-throughput proteomics using antibody microarrays: an update
(2007) In Expert Review of Molecular Diagnostics 7(5). p.673-686- Abstract
- Antibody-based microarrays are a rapidly emerging technology that has advanced from the first proof-of-concept studies to demanding serum protein profiling applications during recent years, displaying great promise within disease proteomics. Miniaturized micro- and nanoarrays can be fabricated with an almost infinite number of antibodies carrying the desired specificities. While consuming only minute amounts of reagents, multiplexed and ultrasensitive assays can be performed targeting high- as well as low-abundance analytes in complex nonfractionated proteomes. The microarray images generated can then be converted into protein expression profiles or protein atlases, revealing a detailed composition of the sample. The technology will... (More)
- Antibody-based microarrays are a rapidly emerging technology that has advanced from the first proof-of-concept studies to demanding serum protein profiling applications during recent years, displaying great promise within disease proteomics. Miniaturized micro- and nanoarrays can be fabricated with an almost infinite number of antibodies carrying the desired specificities. While consuming only minute amounts of reagents, multiplexed and ultrasensitive assays can be performed targeting high- as well as low-abundance analytes in complex nonfractionated proteomes. The microarray images generated can then be converted into protein expression profiles or protein atlases, revealing a detailed composition of the sample. The technology will provide unique opportunities for fields such as disease diagnostics, biomarker discovery, patient stratification, predicting disease recurrence and drug target discovery. This review describes an update of high-throughput proteomics, using antibody-based microarrays, focusing on key technological advances and novel applications that have emerged over the last 3 years. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/654052
- author
- Borrebaeck, Carl LU and Wingren, Christer LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- recombinant, proteomics, protein array, oncoproteomics, nanoarrays, disease proteomics, affinity proteomics, antibody microarrays, antibody microarray
- in
- Expert Review of Molecular Diagnostics
- volume
- 7
- issue
- 5
- pages
- 673 - 686
- publisher
- Future Drugs Ltd
- external identifiers
-
- wos:000250321400020
- scopus:35148842343
- ISSN
- 1744-8352
- DOI
- 10.1586/14737159.7.5.673
- language
- English
- LU publication?
- yes
- id
- c181473e-8bce-47c9-8865-54ff7fed6cb8 (old id 654052)
- date added to LUP
- 2016-04-01 12:38:10
- date last changed
- 2022-01-27 07:45:01
@article{c181473e-8bce-47c9-8865-54ff7fed6cb8,
abstract = {{Antibody-based microarrays are a rapidly emerging technology that has advanced from the first proof-of-concept studies to demanding serum protein profiling applications during recent years, displaying great promise within disease proteomics. Miniaturized micro- and nanoarrays can be fabricated with an almost infinite number of antibodies carrying the desired specificities. While consuming only minute amounts of reagents, multiplexed and ultrasensitive assays can be performed targeting high- as well as low-abundance analytes in complex nonfractionated proteomes. The microarray images generated can then be converted into protein expression profiles or protein atlases, revealing a detailed composition of the sample. The technology will provide unique opportunities for fields such as disease diagnostics, biomarker discovery, patient stratification, predicting disease recurrence and drug target discovery. This review describes an update of high-throughput proteomics, using antibody-based microarrays, focusing on key technological advances and novel applications that have emerged over the last 3 years.}},
author = {{Borrebaeck, Carl and Wingren, Christer}},
issn = {{1744-8352}},
keywords = {{recombinant; proteomics; protein array; oncoproteomics; nanoarrays; disease proteomics; affinity proteomics; antibody microarrays; antibody microarray}},
language = {{eng}},
number = {{5}},
pages = {{673--686}},
publisher = {{Future Drugs Ltd}},
series = {{Expert Review of Molecular Diagnostics}},
title = {{High-throughput proteomics using antibody microarrays: an update}},
url = {{http://dx.doi.org/10.1586/14737159.7.5.673}},
doi = {{10.1586/14737159.7.5.673}},
volume = {{7}},
year = {{2007}},
}