Quantitative and qualitative evaluation of plasma and urine alpha(1)-microglobulin in healthy donors and patients with different haemolytic disorders and haemochromatosis
Nordberg, Jonas LU ; Allhorn, Maria LU ; Winqvist, Ingemar ; Åkerström, Bo LU and Olsson, Martin LU
(2007)
In Clinica Chimica Acta
386(1-2).
p.31-37
- Abstract
- Background: The haem-binding protein alpha(1)-microglobulin (alpha(1)m) is involved in protection against oxidative damage induced by extracellular haem/haemoglobin. A carboxy-terminally truncated form of alpha(1)m (t-alpha(1)m), formed by reactions with haemoglobin, degrades haem into a yellow-brown chromophore linked to the protein. The aim of this work was to investigate if t-alpha(1) in is present in urine from a large cohort and if urinary and plasma alpha(1)m/t-alpha(1)m concentrations are changed in patients with haemolytic disorders and haemochromatosis. Methods: Urine and blood from patients (n = 20) and a control group (n = 22) were investigated for alpha(1) in and t-alpha(1)m by gel electrophoresis, Western blotting and... (More)
- Background: The haem-binding protein alpha(1)-microglobulin (alpha(1)m) is involved in protection against oxidative damage induced by extracellular haem/haemoglobin. A carboxy-terminally truncated form of alpha(1)m (t-alpha(1)m), formed by reactions with haemoglobin, degrades haem into a yellow-brown chromophore linked to the protein. The aim of this work was to investigate if t-alpha(1) in is present in urine from a large cohort and if urinary and plasma alpha(1)m/t-alpha(1)m concentrations are changed in patients with haemolytic disorders and haemochromatosis. Methods: Urine and blood from patients (n = 20) and a control group (n = 22) were investigated for alpha(1) in and t-alpha(1)m by gel electrophoresis, Western blotting and radioimmunoassay. Data were compared to clinical chemistry data and medical records. Results: Two thirds of all studied subjects displayed t-alpha(1)m in urine but the t-alpha(1)m/alpha(1)m ratio was not increased in patients. Instead, significantly elevated ratios were found in females compared to males. Patients with intravascular or extravascular haemolysis showed higher alpha(1)m, albumin and beta(2)-microglobulin/creatinine ratios in urine indicating glomerulo-tubular dysfunction. Conclusions: The demonstration of t-alpha(1)m in urine of this cohort may be of importance in quantitative clinical chemistry. Whilst impaired kidney function due to intravascular haemolysis is well-known to occur, it is an unexpected finding in a group of patients with extravascular haemolysis. (C) 2007 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/655400
- author
- Nordberg, Jonas
LU
; Allhorn, Maria
LU
; Winqvist, Ingemar
; Åkerström, Bo
LU
and Olsson, Martin
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- alpha-l-microglobulin, lipocalins, protein HC, kidney, haemolysis
- in
- Clinica Chimica Acta
- volume
- 386
- issue
- 1-2
- pages
- 31 - 37
- publisher
- Elsevier
- external identifiers
-
- wos:000250195700006
- scopus:34548680086
- ISSN
- 0009-8981
- DOI
- 10.1016/j.cca.2007.07.017
- language
- English
- LU publication?
- yes
- id
- a75c1c5b-e9f2-48a0-9a25-d658efced49d (old id 655400)
- date added to LUP
- 2016-04-01 11:35:44
- date last changed
- 2022-02-25 18:34:40
@article{a75c1c5b-e9f2-48a0-9a25-d658efced49d,
abstract = {{Background: The haem-binding protein alpha(1)-microglobulin (alpha(1)m) is involved in protection against oxidative damage induced by extracellular haem/haemoglobin. A carboxy-terminally truncated form of alpha(1)m (t-alpha(1)m), formed by reactions with haemoglobin, degrades haem into a yellow-brown chromophore linked to the protein. The aim of this work was to investigate if t-alpha(1) in is present in urine from a large cohort and if urinary and plasma alpha(1)m/t-alpha(1)m concentrations are changed in patients with haemolytic disorders and haemochromatosis. Methods: Urine and blood from patients (n = 20) and a control group (n = 22) were investigated for alpha(1) in and t-alpha(1)m by gel electrophoresis, Western blotting and radioimmunoassay. Data were compared to clinical chemistry data and medical records. Results: Two thirds of all studied subjects displayed t-alpha(1)m in urine but the t-alpha(1)m/alpha(1)m ratio was not increased in patients. Instead, significantly elevated ratios were found in females compared to males. Patients with intravascular or extravascular haemolysis showed higher alpha(1)m, albumin and beta(2)-microglobulin/creatinine ratios in urine indicating glomerulo-tubular dysfunction. Conclusions: The demonstration of t-alpha(1)m in urine of this cohort may be of importance in quantitative clinical chemistry. Whilst impaired kidney function due to intravascular haemolysis is well-known to occur, it is an unexpected finding in a group of patients with extravascular haemolysis. (C) 2007 Elsevier B.V. All rights reserved.}},
author = {{Nordberg, Jonas and Allhorn, Maria and Winqvist, Ingemar and Åkerström, Bo and Olsson, Martin}},
issn = {{0009-8981}},
keywords = {{alpha-l-microglobulin; lipocalins; protein HC; kidney; haemolysis}},
language = {{eng}},
number = {{1-2}},
pages = {{31--37}},
publisher = {{Elsevier}},
series = {{Clinica Chimica Acta}},
title = {{Quantitative and qualitative evaluation of plasma and urine alpha(1)-microglobulin in healthy donors and patients with different haemolytic disorders and haemochromatosis}},
url = {{http://dx.doi.org/10.1016/j.cca.2007.07.017}},
doi = {{10.1016/j.cca.2007.07.017}},
volume = {{386}},
year = {{2007}},
}