Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes
(2016) In Epigenomics 8(12). p.1601-1617- Abstract
Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire... (More)
Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.
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- author
- Broholm, Christa ; Olsson, Anders Henrik ; Perfilyev, Alexander LU ; Gillberg, Linn LU ; Hansen, Ninna Schiøler ; Ali, Ashfaq LU ; Mortensen, Brynjulf ; Ling, Charlotte LU and Vaag, Allan LU
- organization
- publishing date
- 2016-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- adipogenesis, adiponectin, DNA methylation, epigenetics, stem cells
- in
- Epigenomics
- volume
- 8
- issue
- 12
- pages
- 17 pages
- publisher
- Future Medicine Ltd.
- external identifiers
-
- scopus:85002944734
- pmid:27854126
- wos:000390675400004
- ISSN
- 1750-1911
- DOI
- 10.2217/epi-2016-0077
- language
- English
- LU publication?
- yes
- id
- 655811c8-e449-4214-8138-959205118aeb
- date added to LUP
- 2016-12-28 10:41:04
- date last changed
- 2024-07-27 01:34:18
@article{655811c8-e449-4214-8138-959205118aeb, abstract = {{<p>Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.</p>}}, author = {{Broholm, Christa and Olsson, Anders Henrik and Perfilyev, Alexander and Gillberg, Linn and Hansen, Ninna Schiøler and Ali, Ashfaq and Mortensen, Brynjulf and Ling, Charlotte and Vaag, Allan}}, issn = {{1750-1911}}, keywords = {{adipogenesis; adiponectin; DNA methylation; epigenetics; stem cells}}, language = {{eng}}, month = {{12}}, number = {{12}}, pages = {{1601--1617}}, publisher = {{Future Medicine Ltd.}}, series = {{Epigenomics}}, title = {{Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes}}, url = {{http://dx.doi.org/10.2217/epi-2016-0077}}, doi = {{10.2217/epi-2016-0077}}, volume = {{8}}, year = {{2016}}, }