Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

Broholm, Christa ; Olsson, Anders Henrik ; Perfilyev, Alexander LU orcid ; Gillberg, Linn LU ; Hansen, Ninna Schiøler ; Ali, Ashfaq LU orcid ; Mortensen, Brynjulf ; Ling, Charlotte LU orcid and Vaag, Allan LU (2016) In Epigenomics 8(12). p.1601-1617
Abstract

Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire... (More)

Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adipogenesis, adiponectin, DNA methylation, epigenetics, stem cells
in
Epigenomics
volume
8
issue
12
pages
17 pages
publisher
Future Medicine Ltd.
external identifiers
  • scopus:85002944734
  • pmid:27854126
  • wos:000390675400004
ISSN
1750-1911
DOI
10.2217/epi-2016-0077
language
English
LU publication?
yes
id
655811c8-e449-4214-8138-959205118aeb
date added to LUP
2016-12-28 10:41:04
date last changed
2024-06-28 22:26:54
@article{655811c8-e449-4214-8138-959205118aeb,
  abstract     = {{<p>Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.</p>}},
  author       = {{Broholm, Christa and Olsson, Anders Henrik and Perfilyev, Alexander and Gillberg, Linn and Hansen, Ninna Schiøler and Ali, Ashfaq and Mortensen, Brynjulf and Ling, Charlotte and Vaag, Allan}},
  issn         = {{1750-1911}},
  keywords     = {{adipogenesis; adiponectin; DNA methylation; epigenetics; stem cells}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{12}},
  pages        = {{1601--1617}},
  publisher    = {{Future Medicine Ltd.}},
  series       = {{Epigenomics}},
  title        = {{Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes}},
  url          = {{http://dx.doi.org/10.2217/epi-2016-0077}},
  doi          = {{10.2217/epi-2016-0077}},
  volume       = {{8}},
  year         = {{2016}},
}