Human trophoblast requires galectin-3 for cell migration and invasion
(2019) In Scientific Reports 9(1).- Abstract
Invasive extravillous cytotrophoblast of the human placenta expresses galectins-1, -3, and -8 in vivo and in vitro. This study aimed to investigate the potential role of galectin-3 in cell migration and invasion, using recombinant human galectin-3 (rhgalectin-3), small molecule galectin inhibitor I 47 , and galectin-3 silencing. HTR-8/SVneo cell migration was stimulated by rhgalectin-3 and reduced by I 47 , which could be neutralised by rhgalectin-3. Inhibitor specificity and selectivity for the galectins expressed in extravillous trophoblast were validated in solid phase... (More)
Invasive extravillous cytotrophoblast of the human placenta expresses galectins-1, -3, and -8 in vivo and in vitro. This study aimed to investigate the potential role of galectin-3 in cell migration and invasion, using recombinant human galectin-3 (rhgalectin-3), small molecule galectin inhibitor I 47 , and galectin-3 silencing. HTR-8/SVneo cell migration was stimulated by rhgalectin-3 and reduced by I 47 , which could be neutralised by rhgalectin-3. Inhibitor specificity and selectivity for the galectins expressed in extravillous trophoblast were validated in solid phase assays using recombinant galectin-1, -3, -8, confirming selectivity for galectin-3. HTR-8/SVneo cell migration and invasion, and invasion by isolated trophoblast cells in primary culture were significantly reduced in the presence of I 47, which could be restored by rhgalectin-3. Upon HTR-8/SVneo cell treatment with galectin-3 siRNA both LGALS3 and galectin-3 protein were dramatically decreased. Silencing of galectin-3 induced significant reduction in cell migration and invasion, which was restored by rhgalectin-3. The influence on known mediators of cell invasion, MMP2 and -9, and integrins α 1 , α 5 , and β 1 was followed in silenced cells, showing lower levels of MMPs and a large reduction in integrin subunit β 1 . These results show that galectin-3 acts as a pro-invasive autocrine/paracrine factor in trophoblast in vitro.
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- author
- Bojić-Trbojević ; Jovanović Krivokuća, M. ; Vilotić, A. ; Kolundžić, N. ; Stefanoska, I. ; Zetterberg, F. ; Nilsson, U. J. LU ; Leffler, H. LU and Vićovac, Lj
- organization
- publishing date
- 2019-02-14
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 9
- issue
- 1
- article number
- 2136
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85061616715
- pmid:30765738
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-018-38374-w
- language
- English
- LU publication?
- yes
- id
- 682e520c-2b9a-43c3-a170-c4c97291e18a
- date added to LUP
- 2019-02-25 10:17:14
- date last changed
- 2024-04-30 01:39:34
@article{682e520c-2b9a-43c3-a170-c4c97291e18a,
abstract = {{<p> Invasive extravillous cytotrophoblast of the human placenta expresses galectins-1, -3, and -8 in vivo and in vitro. This study aimed to investigate the potential role of galectin-3 in cell migration and invasion, using recombinant human galectin-3 (rhgalectin-3), small molecule galectin inhibitor I <sub>47</sub> , and galectin-3 silencing. HTR-8/SVneo cell migration was stimulated by rhgalectin-3 and reduced by I <sub>47</sub> , which could be neutralised by rhgalectin-3. Inhibitor specificity and selectivity for the galectins expressed in extravillous trophoblast were validated in solid phase assays using recombinant galectin-1, -3, -8, confirming selectivity for galectin-3. HTR-8/SVneo cell migration and invasion, and invasion by isolated trophoblast cells in primary culture were significantly reduced in the presence of I <sub>47,</sub> which could be restored by rhgalectin-3. Upon HTR-8/SVneo cell treatment with galectin-3 siRNA both LGALS3 and galectin-3 protein were dramatically decreased. Silencing of galectin-3 induced significant reduction in cell migration and invasion, which was restored by rhgalectin-3. The influence on known mediators of cell invasion, MMP2 and -9, and integrins α <sub>1</sub> , α <sub>5</sub> , and β <sub>1</sub> was followed in silenced cells, showing lower levels of MMPs and a large reduction in integrin subunit β <sub>1</sub> . These results show that galectin-3 acts as a pro-invasive autocrine/paracrine factor in trophoblast in vitro. </p>}},
author = {{Bojić-Trbojević and Jovanović Krivokuća, M. and Vilotić, A. and Kolundžić, N. and Stefanoska, I. and Zetterberg, F. and Nilsson, U. J. and Leffler, H. and Vićovac, Lj}},
issn = {{2045-2322}},
language = {{eng}},
month = {{02}},
number = {{1}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Human trophoblast requires galectin-3 for cell migration and invasion}},
url = {{http://dx.doi.org/10.1038/s41598-018-38374-w}},
doi = {{10.1038/s41598-018-38374-w}},
volume = {{9}},
year = {{2019}},
}