Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Miniaturised expanded-bed column with low dispersion suitable for fast flow-ELISA analyses

Pålsson, Eva ; Nandakumar, M. P. ; Mattiasson, Bo LU and Larsson, Per-Olof LU (2000) In Biotechnology Letters 22. p.245-250
Abstract
Flow-ELISA measurements of the monoclonal antibody concentration in
cultivation broth containing murine hybriboma cells were carried out
using a small expanded-bed column (0.5×2.5 cm) charged with protein A. A
new specialised pellicular agarose/stainless steel matrix designed for
high flow rates with fast mass transport properties was used. Special
care was taken to get an efficient flow distribution. The axial
dispersion coefficient was very low (2×10−6 m2 s−1 for latex particles at a linear velocity of 10 cm min−1). Breakthrough curves for polyclonal IgG on the protein A-derivatised support (at 2–11 cm min−1)
further emphasised its advantageous... (More)
Flow-ELISA measurements of the monoclonal antibody concentration in
cultivation broth containing murine hybriboma cells were carried out
using a small expanded-bed column (0.5×2.5 cm) charged with protein A. A
new specialised pellicular agarose/stainless steel matrix designed for
high flow rates with fast mass transport properties was used. Special
care was taken to get an efficient flow distribution. The axial
dispersion coefficient was very low (2×10−6 m2 s−1 for latex particles at a linear velocity of 10 cm min−1). Breakthrough curves for polyclonal IgG on the protein A-derivatised support (at 2–11 cm min−1)
further emphasised its advantageous properties. No significant change
in dynamic capacity was found over the entire speed range. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Monoclonal antibodies, Protein A, Stainless steel - agarose
in
Biotechnology Letters
volume
22
pages
6 pages
publisher
Springer
external identifiers
  • scopus:0043037604
ISSN
0141-5492
DOI
10.1023/A:1005693120337
language
English
LU publication?
yes
id
683e6b94-c794-406f-91dd-98b66087ec55
date added to LUP
2024-05-17 21:22:04
date last changed
2024-05-24 13:48:13
@article{683e6b94-c794-406f-91dd-98b66087ec55,
  abstract     = {{Flow-ELISA measurements of the monoclonal antibody concentration in <br>
cultivation broth containing murine hybriboma cells were carried out <br>
using a small expanded-bed column (0.5×2.5 cm) charged with protein A. A<br>
 new specialised pellicular agarose/stainless steel matrix designed for <br>
high flow rates with fast mass transport properties was used. Special <br>
care was taken to get an efficient flow distribution. The axial <br>
dispersion coefficient was very low (2×10<sup>−6</sup> m<sup>2</sup> s<sup>−1</sup> for latex particles at a linear velocity of 10 cm min<sup>−1</sup>). Breakthrough curves for polyclonal IgG on the protein A-derivatised support (at 2–11 cm min<sup>−1</sup>)<br>
 further emphasised its advantageous properties. No significant change <br>
in dynamic capacity was found over the entire speed range.}},
  author       = {{Pålsson, Eva and Nandakumar, M. P. and Mattiasson, Bo and Larsson, Per-Olof}},
  issn         = {{0141-5492}},
  keywords     = {{Monoclonal antibodies; Protein A; Stainless steel - agarose}},
  language     = {{eng}},
  pages        = {{245--250}},
  publisher    = {{Springer}},
  series       = {{Biotechnology Letters}},
  title        = {{Miniaturised expanded-bed column with low dispersion suitable for fast flow-ELISA analyses}},
  url          = {{http://dx.doi.org/10.1023/A:1005693120337}},
  doi          = {{10.1023/A:1005693120337}},
  volume       = {{22}},
  year         = {{2000}},
}