Miniaturised expanded-bed column with low dispersion suitable for fast flow-ELISA analyses
(2000) In Biotechnology Letters 22. p.245-250- Abstract
- Flow-ELISA measurements of the monoclonal antibody concentration in
cultivation broth containing murine hybriboma cells were carried out
using a small expanded-bed column (0.5×2.5 cm) charged with protein A. A
new specialised pellicular agarose/stainless steel matrix designed for
high flow rates with fast mass transport properties was used. Special
care was taken to get an efficient flow distribution. The axial
dispersion coefficient was very low (2×10−6 m2 s−1 for latex particles at a linear velocity of 10 cm min−1). Breakthrough curves for polyclonal IgG on the protein A-derivatised support (at 2–11 cm min−1)
further emphasised its advantageous... (More) - Flow-ELISA measurements of the monoclonal antibody concentration in
cultivation broth containing murine hybriboma cells were carried out
using a small expanded-bed column (0.5×2.5 cm) charged with protein A. A
new specialised pellicular agarose/stainless steel matrix designed for
high flow rates with fast mass transport properties was used. Special
care was taken to get an efficient flow distribution. The axial
dispersion coefficient was very low (2×10−6 m2 s−1 for latex particles at a linear velocity of 10 cm min−1). Breakthrough curves for polyclonal IgG on the protein A-derivatised support (at 2–11 cm min−1)
further emphasised its advantageous properties. No significant change
in dynamic capacity was found over the entire speed range. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/683e6b94-c794-406f-91dd-98b66087ec55
- author
- Pålsson, Eva ; Nandakumar, M. P. ; Mattiasson, Bo LU and Larsson, Per-Olof LU
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Monoclonal antibodies, Protein A, Stainless steel - agarose
- in
- Biotechnology Letters
- volume
- 22
- pages
- 6 pages
- publisher
- Springer
- external identifiers
-
- scopus:0043037604
- ISSN
- 0141-5492
- DOI
- 10.1023/A:1005693120337
- language
- English
- LU publication?
- yes
- id
- 683e6b94-c794-406f-91dd-98b66087ec55
- date added to LUP
- 2024-05-17 21:22:04
- date last changed
- 2024-05-24 13:48:13
@article{683e6b94-c794-406f-91dd-98b66087ec55, abstract = {{Flow-ELISA measurements of the monoclonal antibody concentration in <br> cultivation broth containing murine hybriboma cells were carried out <br> using a small expanded-bed column (0.5×2.5 cm) charged with protein A. A<br> new specialised pellicular agarose/stainless steel matrix designed for <br> high flow rates with fast mass transport properties was used. Special <br> care was taken to get an efficient flow distribution. The axial <br> dispersion coefficient was very low (2×10<sup>−6</sup> m<sup>2</sup> s<sup>−1</sup> for latex particles at a linear velocity of 10 cm min<sup>−1</sup>). Breakthrough curves for polyclonal IgG on the protein A-derivatised support (at 2–11 cm min<sup>−1</sup>)<br> further emphasised its advantageous properties. No significant change <br> in dynamic capacity was found over the entire speed range.}}, author = {{Pålsson, Eva and Nandakumar, M. P. and Mattiasson, Bo and Larsson, Per-Olof}}, issn = {{0141-5492}}, keywords = {{Monoclonal antibodies; Protein A; Stainless steel - agarose}}, language = {{eng}}, pages = {{245--250}}, publisher = {{Springer}}, series = {{Biotechnology Letters}}, title = {{Miniaturised expanded-bed column with low dispersion suitable for fast flow-ELISA analyses}}, url = {{http://dx.doi.org/10.1023/A:1005693120337}}, doi = {{10.1023/A:1005693120337}}, volume = {{22}}, year = {{2000}}, }