Characterization of the transcriptional and functional effects of fibroblast growth factor-1 on human preadipocyte differentiation
(2006) In FASEB Journal 20(14). p.2615-2615- Abstract
- We recently established that fibroblast growth factor (FGF)-1 promotes adipogenesis of primary human preadipocytes (phPA). In the current report, we have characterized the adipogenic effects of FGF-1 in phPA and also in a human PA strain derived from an individual with Simpson-Golabi-Behmel syndrome (SGBS PA), which exhibit an intrinsic capacity to differentiate with high efficiency. In further studies, we compared these models with the well-characterized murine 3T3-L1 preadipocyte cell line (3T3-L1 PA). FGF-1 up-regulated the adipogenic program in phPA, with increased expression of peroxisome proliferator-activated receptor-gamma in confluent PA prior to induction of differentiation and increased expression of adipocyte markers during... (More)
- We recently established that fibroblast growth factor (FGF)-1 promotes adipogenesis of primary human preadipocytes (phPA). In the current report, we have characterized the adipogenic effects of FGF-1 in phPA and also in a human PA strain derived from an individual with Simpson-Golabi-Behmel syndrome (SGBS PA), which exhibit an intrinsic capacity to differentiate with high efficiency. In further studies, we compared these models with the well-characterized murine 3T3-L1 preadipocyte cell line (3T3-L1 PA). FGF-1 up-regulated the adipogenic program in phPA, with increased expression of peroxisome proliferator-activated receptor-gamma in confluent PA prior to induction of differentiation and increased expression of adipocyte markers during differentiation. Moreover, phPA differentiated in the presence of FGF-1 were more insulin responsive and secreted increased levels of adiponectin. FGF-1 treatment of SGBS PA further enhanced differentiation. For the most part, the adipogenic program in phPA paralleled that observed in 3T3-L1 PA; however, we found no evidence of mitotic clonal expansion in the phPA. Finally, we investigated a role for extracellular regulated kinase 1/2 (ERK 1/2) in adipogenesis of phPA. FGF-1 induced robust phosphorylation of ERK1/2 in early differentiation and inhibition of ERK1/2 activity significantly reduced phPA differentiation. These data suggest that FGF-1 treated phPA represent a valuable in vitro model for the study of adipogenesis and insulin action and indicate that ERK1/2 activation is necessary for human adipogenesis in the absence of mitotic clonal expansion. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/685205
- author
- Newell, Felicity S. ; Su, Hua ; Tornqvist, Hans LU ; Whitehead, Jonathan P. ; Prins, Johannes B. and Hutley, Louise J.
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- obesity, adipogenesis, mitosis
- in
- FASEB Journal
- volume
- 20
- issue
- 14
- pages
- 2615 - 2615
- publisher
- Wiley
- external identifiers
-
- wos:000242490700042
- scopus:33845611030
- ISSN
- 1530-6860
- DOI
- 10.1096/fj.05-5710fje
- language
- English
- LU publication?
- yes
- id
- 53d3ca4d-684d-49ff-9c77-005bb0bb267d (old id 685205)
- date added to LUP
- 2016-04-01 16:19:34
- date last changed
- 2024-02-09 21:29:14
@article{53d3ca4d-684d-49ff-9c77-005bb0bb267d,
abstract = {{We recently established that fibroblast growth factor (FGF)-1 promotes adipogenesis of primary human preadipocytes (phPA). In the current report, we have characterized the adipogenic effects of FGF-1 in phPA and also in a human PA strain derived from an individual with Simpson-Golabi-Behmel syndrome (SGBS PA), which exhibit an intrinsic capacity to differentiate with high efficiency. In further studies, we compared these models with the well-characterized murine 3T3-L1 preadipocyte cell line (3T3-L1 PA). FGF-1 up-regulated the adipogenic program in phPA, with increased expression of peroxisome proliferator-activated receptor-gamma in confluent PA prior to induction of differentiation and increased expression of adipocyte markers during differentiation. Moreover, phPA differentiated in the presence of FGF-1 were more insulin responsive and secreted increased levels of adiponectin. FGF-1 treatment of SGBS PA further enhanced differentiation. For the most part, the adipogenic program in phPA paralleled that observed in 3T3-L1 PA; however, we found no evidence of mitotic clonal expansion in the phPA. Finally, we investigated a role for extracellular regulated kinase 1/2 (ERK 1/2) in adipogenesis of phPA. FGF-1 induced robust phosphorylation of ERK1/2 in early differentiation and inhibition of ERK1/2 activity significantly reduced phPA differentiation. These data suggest that FGF-1 treated phPA represent a valuable in vitro model for the study of adipogenesis and insulin action and indicate that ERK1/2 activation is necessary for human adipogenesis in the absence of mitotic clonal expansion.}},
author = {{Newell, Felicity S. and Su, Hua and Tornqvist, Hans and Whitehead, Jonathan P. and Prins, Johannes B. and Hutley, Louise J.}},
issn = {{1530-6860}},
keywords = {{obesity; adipogenesis; mitosis}},
language = {{eng}},
number = {{14}},
pages = {{2615--2615}},
publisher = {{Wiley}},
series = {{FASEB Journal}},
title = {{Characterization of the transcriptional and functional effects of fibroblast growth factor-1 on human preadipocyte differentiation}},
url = {{http://dx.doi.org/10.1096/fj.05-5710fje}},
doi = {{10.1096/fj.05-5710fje}},
volume = {{20}},
year = {{2006}},
}