The functional interaction of EGF and PDGF with bradykinin in the proliferation of human gingival fibroblasts.
(1995) In Journal of Periodontology 66(6). p.429-437- Abstract
Epidermal growth factor (EGF) and platelet-derived growth factor (PDGF)-BB are both involved in periodontal wound healing. Each of these growth factors exerts a positive proliferative effect on cells of the periodontium in vitro. However, in vivo the peptide bradykinin is one of a complex array of mediators present in addition to these growth factors. The purposes of this investigation were to: 1) evaluate bradykinin interactions with EGF and PDGF-BB altering cell proliferation in cultured human gingival fibroblasts (HGF), periodontal ligament cells (HPDL), and cells derived from alveolar bone (HOB); and 2) determine at the signal transduction level the mechanism of interaction between EGF and bradykinin in HGF. EGF and PDGF-BB... (More)
Epidermal growth factor (EGF) and platelet-derived growth factor (PDGF)-BB are both involved in periodontal wound healing. Each of these growth factors exerts a positive proliferative effect on cells of the periodontium in vitro. However, in vivo the peptide bradykinin is one of a complex array of mediators present in addition to these growth factors. The purposes of this investigation were to: 1) evaluate bradykinin interactions with EGF and PDGF-BB altering cell proliferation in cultured human gingival fibroblasts (HGF), periodontal ligament cells (HPDL), and cells derived from alveolar bone (HOB); and 2) determine at the signal transduction level the mechanism of interaction between EGF and bradykinin in HGF. EGF and PDGF-BB stimulated DNA synthesis in a concentration-dependent manner, as measured by [3H] thymidine incorporation. Bradykinin alone did not alter significantly based DNA synthesis values; however, bradykinin in combination with EGF reduced DNA synthesis to nearly basal levels and bradykinin in combination with PDGF reduced the DNA synthesis over 50%. Examination of the interactions between bradykinin and EGF signal transduction pathways revealed that PGE2 release was increased in the presence of bradykinin and EGF (167 +/- 33% to 317 +/- 29%). The bradykinin-stimulated PGE2 release was completely abolished by indomethacin. Indomethacin also was found to block the bradykinin inhibition of EGF-induced DNA synthesis.(ABSTRACT TRUNCATED AT 250 WORDS)
(Less)
- author
- McAllister, B. S. ; Leeb-Lundberg, F. LU ; Mellonig, J. T. and Olson, M. S.
- publishing date
- 1995-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Periodontology
- volume
- 66
- issue
- 6
- pages
- 429 - 437
- publisher
- American Academy of Periodontology
- external identifiers
-
- scopus:0029320264
- pmid:7562331
- ISSN
- 0022-3492
- DOI
- 10.1902/jop.1995.66.6.429
- language
- English
- LU publication?
- no
- id
- 6e0bc670-73e5-433d-9da2-a1412a786f15
- date added to LUP
- 2019-06-12 11:38:44
- date last changed
- 2024-07-24 20:56:59
@article{6e0bc670-73e5-433d-9da2-a1412a786f15, abstract = {{<p>Epidermal growth factor (EGF) and platelet-derived growth factor (PDGF)-BB are both involved in periodontal wound healing. Each of these growth factors exerts a positive proliferative effect on cells of the periodontium in vitro. However, in vivo the peptide bradykinin is one of a complex array of mediators present in addition to these growth factors. The purposes of this investigation were to: 1) evaluate bradykinin interactions with EGF and PDGF-BB altering cell proliferation in cultured human gingival fibroblasts (HGF), periodontal ligament cells (HPDL), and cells derived from alveolar bone (HOB); and 2) determine at the signal transduction level the mechanism of interaction between EGF and bradykinin in HGF. EGF and PDGF-BB stimulated DNA synthesis in a concentration-dependent manner, as measured by [3H] thymidine incorporation. Bradykinin alone did not alter significantly based DNA synthesis values; however, bradykinin in combination with EGF reduced DNA synthesis to nearly basal levels and bradykinin in combination with PDGF reduced the DNA synthesis over 50%. Examination of the interactions between bradykinin and EGF signal transduction pathways revealed that PGE2 release was increased in the presence of bradykinin and EGF (167 +/- 33% to 317 +/- 29%). The bradykinin-stimulated PGE2 release was completely abolished by indomethacin. Indomethacin also was found to block the bradykinin inhibition of EGF-induced DNA synthesis.(ABSTRACT TRUNCATED AT 250 WORDS)</p>}}, author = {{McAllister, B. S. and Leeb-Lundberg, F. and Mellonig, J. T. and Olson, M. S.}}, issn = {{0022-3492}}, language = {{eng}}, month = {{01}}, number = {{6}}, pages = {{429--437}}, publisher = {{American Academy of Periodontology}}, series = {{Journal of Periodontology}}, title = {{The functional interaction of EGF and PDGF with bradykinin in the proliferation of human gingival fibroblasts.}}, url = {{http://dx.doi.org/10.1902/jop.1995.66.6.429}}, doi = {{10.1902/jop.1995.66.6.429}}, volume = {{66}}, year = {{1995}}, }