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ARID1a Associates with Lymphoid-Restricted Transcription Factors and Has an Essential Role in T Cell Development

Astori, Audrey ; Tingvall-Gustafsson, Johanna LU orcid ; Kuruvilla, Jacob LU ; Coyaud, Etienne ; Laurent, Estelle M.N. ; Sunnerhagen, Maria ; Åhsberg, Josefine LU ; Ungerbäck, Jonas LU ; Strid, Tobias LU and Sigvardsson, Mikael LU , et al. (2020) In Journal of immunology 205(5). p.1419-1432
Abstract

Maturation of lymphoid cells is controlled by the action of stage and lineage-restricted transcription factors working in concert with the general transcription and chromatin remodeling machinery to regulate gene expression. To better understand this functional interplay, we used Biotin Identification in human embryonic kidney cells to identify proximity interaction partners for GATA3, TCF7 (TCF1), SPI1, HLF, IKZF1, PAX5, ID1, and ID2. The proximity interaction partners shared among the lineage-restricted transcription factors included ARID1a, a BRG1-associated factor complex component. CUT&RUN analysis revealed that ARID1a shared binding with TCF7 and GATA3 at a substantial number of putative regulatory elements in mouse T cell... (More)

Maturation of lymphoid cells is controlled by the action of stage and lineage-restricted transcription factors working in concert with the general transcription and chromatin remodeling machinery to regulate gene expression. To better understand this functional interplay, we used Biotin Identification in human embryonic kidney cells to identify proximity interaction partners for GATA3, TCF7 (TCF1), SPI1, HLF, IKZF1, PAX5, ID1, and ID2. The proximity interaction partners shared among the lineage-restricted transcription factors included ARID1a, a BRG1-associated factor complex component. CUT&RUN analysis revealed that ARID1a shared binding with TCF7 and GATA3 at a substantial number of putative regulatory elements in mouse T cell progenitors. In support of an important function for ARID1a in lymphocyte development, deletion of Arid1a in early lymphoid progenitors in mice resulted in a pronounced developmental arrest in early T cell development with a reduction of CD4+CD8+ cells and a 20-fold reduction in thymic cellularity. Exploring gene expression patterns in DN3 cells from Wt and Arid1a-deficient mice suggested that the developmental block resided in the DN3a to DN3b transition, indicating a deficiency in β-selection. Our work highlights the critical importance of functional interactions between stage and lineage-restricted factors and the basic transcription machinery during lymphocyte differentiation.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of immunology
volume
205
issue
5
pages
14 pages
publisher
American Association of Immunologists
external identifiers
  • scopus:85090072061
  • pmid:32747500
ISSN
1550-6606
DOI
10.4049/jimmunol.1900959
language
English
LU publication?
yes
id
6ea6484b-e460-4f2d-9d00-c72df28e682e
date added to LUP
2020-09-25 10:29:21
date last changed
2024-07-12 01:20:26
@article{6ea6484b-e460-4f2d-9d00-c72df28e682e,
  abstract     = {{<p>Maturation of lymphoid cells is controlled by the action of stage and lineage-restricted transcription factors working in concert with the general transcription and chromatin remodeling machinery to regulate gene expression. To better understand this functional interplay, we used Biotin Identification in human embryonic kidney cells to identify proximity interaction partners for GATA3, TCF7 (TCF1), SPI1, HLF, IKZF1, PAX5, ID1, and ID2. The proximity interaction partners shared among the lineage-restricted transcription factors included ARID1a, a BRG1-associated factor complex component. CUT&amp;RUN analysis revealed that ARID1a shared binding with TCF7 and GATA3 at a substantial number of putative regulatory elements in mouse T cell progenitors. In support of an important function for ARID1a in lymphocyte development, deletion of Arid1a in early lymphoid progenitors in mice resulted in a pronounced developmental arrest in early T cell development with a reduction of CD4+CD8+ cells and a 20-fold reduction in thymic cellularity. Exploring gene expression patterns in DN3 cells from Wt and Arid1a-deficient mice suggested that the developmental block resided in the DN3a to DN3b transition, indicating a deficiency in β-selection. Our work highlights the critical importance of functional interactions between stage and lineage-restricted factors and the basic transcription machinery during lymphocyte differentiation.</p>}},
  author       = {{Astori, Audrey and Tingvall-Gustafsson, Johanna and Kuruvilla, Jacob and Coyaud, Etienne and Laurent, Estelle M.N. and Sunnerhagen, Maria and Åhsberg, Josefine and Ungerbäck, Jonas and Strid, Tobias and Sigvardsson, Mikael and Raught, Brian and Somasundaram, Rajesh}},
  issn         = {{1550-6606}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1419--1432}},
  publisher    = {{American Association of Immunologists}},
  series       = {{Journal of immunology}},
  title        = {{ARID1a Associates with Lymphoid-Restricted Transcription Factors and Has an Essential Role in T Cell Development}},
  url          = {{http://dx.doi.org/10.4049/jimmunol.1900959}},
  doi          = {{10.4049/jimmunol.1900959}},
  volume       = {{205}},
  year         = {{2020}},
}