The bacterial toxin RelE induces specific mRNA cleavage in the A site of the eukaryote ribosome
(2008) In RNA 14(2). p.233-239- Abstract
RelE/RelB is a well-characterized toxin-anti-toxin pair involved in nutritional stress responses in Bacteria and Archae. RelE lacks any eukaryote homolog, but we demonstrate here that it efficiently and specifically cleaves mRNA in the A site of the eukaryote ribosome. The cleavage mechanism is similar to that in bacteria, showing the feasibility of A-site cleavage of mRNA for regulatory purposes also in eukaryotes. RelE cleavage in the A-site codon of a stalled eukaryote ribosome is precise and easily monitored, making "RelE printing" a useful complement to toeprinting to determine the exact mRNA location on the eukaryote ribosome and to probe the occupancy of its A site. Published by Cold Spring Harbor Laboratory Press.
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- author
- Andreev, Dmitri ; Hauryliuk, Vasili LU ; Terenin, Ilya ; Dmitriev, Sergey ; Ehrenberg, Måns and Shatsky, Ivan
- publishing date
- 2008-02
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bacterial toxin RelE, Eukaryotic ribosomes, Initiation complex assembly, mRNA cleavage, RelE printing, Toeprinting
- in
- RNA
- volume
- 14
- issue
- 2
- pages
- 233 - 239
- publisher
- Cold Spring Harbor Laboratory Press (CSHL)
- external identifiers
-
- scopus:38649111610
- pmid:18083838
- ISSN
- 1355-8382
- DOI
- 10.1261/rna.693208
- language
- English
- LU publication?
- no
- additional info
- Copyright: Copyright 2009 Elsevier B.V., All rights reserved.
- id
- 715075fe-29a3-4970-aa20-f78c724147f2
- date added to LUP
- 2021-09-24 20:51:38
- date last changed
- 2024-05-04 13:51:56
@article{715075fe-29a3-4970-aa20-f78c724147f2, abstract = {{<p>RelE/RelB is a well-characterized toxin-anti-toxin pair involved in nutritional stress responses in Bacteria and Archae. RelE lacks any eukaryote homolog, but we demonstrate here that it efficiently and specifically cleaves mRNA in the A site of the eukaryote ribosome. The cleavage mechanism is similar to that in bacteria, showing the feasibility of A-site cleavage of mRNA for regulatory purposes also in eukaryotes. RelE cleavage in the A-site codon of a stalled eukaryote ribosome is precise and easily monitored, making "RelE printing" a useful complement to toeprinting to determine the exact mRNA location on the eukaryote ribosome and to probe the occupancy of its A site. Published by Cold Spring Harbor Laboratory Press.</p>}}, author = {{Andreev, Dmitri and Hauryliuk, Vasili and Terenin, Ilya and Dmitriev, Sergey and Ehrenberg, Måns and Shatsky, Ivan}}, issn = {{1355-8382}}, keywords = {{Bacterial toxin RelE; Eukaryotic ribosomes; Initiation complex assembly; mRNA cleavage; RelE printing; Toeprinting}}, language = {{eng}}, number = {{2}}, pages = {{233--239}}, publisher = {{Cold Spring Harbor Laboratory Press (CSHL)}}, series = {{RNA}}, title = {{The bacterial toxin RelE induces specific mRNA cleavage in the A site of the eukaryote ribosome}}, url = {{http://dx.doi.org/10.1261/rna.693208}}, doi = {{10.1261/rna.693208}}, volume = {{14}}, year = {{2008}}, }