Large crystal growth for neutron protein crystallography
(2020) In Methods in Enzymology 634. p.21-46- Abstract
The use of neutron protein crystallography (NPX) is expanding rapidly, with most structures determined in the last decade. This growth is stimulated by a number of developments, spanning from the building of new NPX beamlines to the availability of improved software for structure refinement. The main bottleneck preventing structural biologists from adding NPX to the suite of methods commonly used is the large volume of the individual crystals required for a successful experiment. A survey of deposited NPX structures in the Protein Data Bank shows that about two-thirds came from crystals prepared using vapor diffusion, while batch and dialysis-based methods all-together contribute to most of the remaining one-third. This chapter explains... (More)
The use of neutron protein crystallography (NPX) is expanding rapidly, with most structures determined in the last decade. This growth is stimulated by a number of developments, spanning from the building of new NPX beamlines to the availability of improved software for structure refinement. The main bottleneck preventing structural biologists from adding NPX to the suite of methods commonly used is the large volume of the individual crystals required for a successful experiment. A survey of deposited NPX structures in the Protein Data Bank shows that about two-thirds came from crystals prepared using vapor diffusion, while batch and dialysis-based methods all-together contribute to most of the remaining one-third. This chapter explains the underlying principles of these protein crystallization methods and provides practical examples that may help others to successfully prepare large crystals for NPX.
(Less)
- author
- Budayova-Spano, Monika ; Koruza, Katarina LU and Fisher, Zoë LU
- organization
- publishing date
- 2020-01-21
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Dialysis, Microseeding, Phase diagram, Vapor diffusion
- host publication
- Neutron Crystallography in Structural Biology
- series title
- Methods in Enzymology
- editor
- Moody, Peter C.E.
- volume
- 634
- pages
- 26 pages
- publisher
- Academic Press
- external identifiers
-
- pmid:32093834
- scopus:85078158714
- ISSN
- 1557-7988
- 0076-6879
- DOI
- 10.1016/bs.mie.2019.11.015
- language
- English
- LU publication?
- yes
- id
- 73ce3e19-bbb1-4cab-b473-66c831ececc3
- date added to LUP
- 2020-02-10 12:45:03
- date last changed
- 2024-08-21 15:38:46
@inbook{73ce3e19-bbb1-4cab-b473-66c831ececc3, abstract = {{<p>The use of neutron protein crystallography (NPX) is expanding rapidly, with most structures determined in the last decade. This growth is stimulated by a number of developments, spanning from the building of new NPX beamlines to the availability of improved software for structure refinement. The main bottleneck preventing structural biologists from adding NPX to the suite of methods commonly used is the large volume of the individual crystals required for a successful experiment. A survey of deposited NPX structures in the Protein Data Bank shows that about two-thirds came from crystals prepared using vapor diffusion, while batch and dialysis-based methods all-together contribute to most of the remaining one-third. This chapter explains the underlying principles of these protein crystallization methods and provides practical examples that may help others to successfully prepare large crystals for NPX.</p>}}, author = {{Budayova-Spano, Monika and Koruza, Katarina and Fisher, Zoë}}, booktitle = {{Neutron Crystallography in Structural Biology}}, editor = {{Moody, Peter C.E.}}, issn = {{1557-7988}}, keywords = {{Dialysis; Microseeding; Phase diagram; Vapor diffusion}}, language = {{eng}}, month = {{01}}, pages = {{21--46}}, publisher = {{Academic Press}}, series = {{Methods in Enzymology}}, title = {{Large crystal growth for neutron protein crystallography}}, url = {{http://dx.doi.org/10.1016/bs.mie.2019.11.015}}, doi = {{10.1016/bs.mie.2019.11.015}}, volume = {{634}}, year = {{2020}}, }