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Bioinspired mycobacterial lipid coating of porous particles for enhanced antimicrobial efficacy

Campos Pacheco, Jesús E. LU ; Davids, Camilla LU ; Yalovenko, Tetiana ; Näsström, Elin ; Ahnlund, Maria ; Godaly, Gabriela LU orcid and Valetti, Sabrina (2025) In European Journal of Pharmaceutical Sciences 213.
Abstract

The study aimed to investigate the unique lipid composition of Mycobacterium bovis BCG and its potential to enhance antimicrobial efficacy of lipid-coated mesoporous silica particles (MSPs). The bacterial lipids (BL) were extracted with petroleum ether and analyzed via LC-MS, revealing a complex mixture of phospholipids, including cardiolipin, phosphatidylcholine, phosphatidylethanolamine, and triacylglycerols. Lipid coating (using bacterial lipids and lung surfactant DPPC as the main component) was performed on MSPs via vesicle fusion approach and confirmed with ATR-FTIR spectroscopy. MSPs were loaded with clofazimine (CLZ), as a drug model for tuberculosis. The obtained BL-DPPC-coated CLZ-MSPs were more effective in inhibiting... (More)

The study aimed to investigate the unique lipid composition of Mycobacterium bovis BCG and its potential to enhance antimicrobial efficacy of lipid-coated mesoporous silica particles (MSPs). The bacterial lipids (BL) were extracted with petroleum ether and analyzed via LC-MS, revealing a complex mixture of phospholipids, including cardiolipin, phosphatidylcholine, phosphatidylethanolamine, and triacylglycerols. Lipid coating (using bacterial lipids and lung surfactant DPPC as the main component) was performed on MSPs via vesicle fusion approach and confirmed with ATR-FTIR spectroscopy. MSPs were loaded with clofazimine (CLZ), as a drug model for tuberculosis. The obtained BL-DPPC-coated CLZ-MSPs were more effective in inhibiting mycobacterial growth and killing intracellular mycobacteria compared to uncoated and DPPC-coated CLZ-MSPs. The bacterial lipids showed a good safety profile on M1-like and M2-like human primary macrophages without inducing a strong immune response or formation of foam cells. These findings suggest that the obtained bacterial lipid coatings can improve antimicrobial efficacy in treating both extracellular and intracellular mycobacteria infections directly in the lungs.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bacterial lipids, Cardiolipin, Clofazimine (CLZ), Lipid coating, Mesoporous silica particles (MSPs), Mycobacterial infections, Mycobacterium bovis BCG
in
European Journal of Pharmaceutical Sciences
volume
213
article number
107225
publisher
Elsevier
external identifiers
  • scopus:105013511124
  • pmid:40780537
ISSN
0928-0987
DOI
10.1016/j.ejps.2025.107225
language
English
LU publication?
yes
id
74a0d2f9-7f66-4d6f-a0ba-49193141f786
date added to LUP
2025-10-13 10:28:08
date last changed
2025-10-14 09:30:43
@article{74a0d2f9-7f66-4d6f-a0ba-49193141f786,
  abstract     = {{<p>The study aimed to investigate the unique lipid composition of Mycobacterium bovis BCG and its potential to enhance antimicrobial efficacy of lipid-coated mesoporous silica particles (MSPs). The bacterial lipids (BL) were extracted with petroleum ether and analyzed via LC-MS, revealing a complex mixture of phospholipids, including cardiolipin, phosphatidylcholine, phosphatidylethanolamine, and triacylglycerols. Lipid coating (using bacterial lipids and lung surfactant DPPC as the main component) was performed on MSPs via vesicle fusion approach and confirmed with ATR-FTIR spectroscopy. MSPs were loaded with clofazimine (CLZ), as a drug model for tuberculosis. The obtained BL-DPPC-coated CLZ-MSPs were more effective in inhibiting mycobacterial growth and killing intracellular mycobacteria compared to uncoated and DPPC-coated CLZ-MSPs. The bacterial lipids showed a good safety profile on M1-like and M2-like human primary macrophages without inducing a strong immune response or formation of foam cells. These findings suggest that the obtained bacterial lipid coatings can improve antimicrobial efficacy in treating both extracellular and intracellular mycobacteria infections directly in the lungs.</p>}},
  author       = {{Campos Pacheco, Jesús E. and Davids, Camilla and Yalovenko, Tetiana and Näsström, Elin and Ahnlund, Maria and Godaly, Gabriela and Valetti, Sabrina}},
  issn         = {{0928-0987}},
  keywords     = {{Bacterial lipids; Cardiolipin; Clofazimine (CLZ); Lipid coating; Mesoporous silica particles (MSPs); Mycobacterial infections; Mycobacterium bovis BCG}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{European Journal of Pharmaceutical Sciences}},
  title        = {{Bioinspired mycobacterial lipid coating of porous particles for enhanced antimicrobial efficacy}},
  url          = {{http://dx.doi.org/10.1016/j.ejps.2025.107225}},
  doi          = {{10.1016/j.ejps.2025.107225}},
  volume       = {{213}},
  year         = {{2025}},
}