HIV-2 Neutralization Sensitivity in Relation to Co-Receptor Entry Pathways and Env Motifs
(2022) In International Journal of Molecular Sciences 23(9).- Abstract
HIV-2, compared to HIV-1, elicits potent and broadly neutralizing antibodies, and uses a broad range of co-receptors. However, both sensitivity to neutralization and breadth of co-receptor use varies between HIV-2 isolates, and the molecular background is still not fully understood. Thus, in the current study, we have deciphered relationships between HIV-2 neutralization sensitivity, co-receptor use and viral envelope glycoprotein (Env) molecular motifs. A panel of primary HIV-2 isolates, with predefined use of co-receptors, was assessed for neutralization sensitivity using a set of HIV-2 Env-directed monoclonal antibodies and co-receptor indicator cell lines. Neutralization sensitivity of the isolates was analysed in relation target... (More)
HIV-2, compared to HIV-1, elicits potent and broadly neutralizing antibodies, and uses a broad range of co-receptors. However, both sensitivity to neutralization and breadth of co-receptor use varies between HIV-2 isolates, and the molecular background is still not fully understood. Thus, in the current study, we have deciphered relationships between HIV-2 neutralization sensitivity, co-receptor use and viral envelope glycoprotein (Env) molecular motifs. A panel of primary HIV-2 isolates, with predefined use of co-receptors, was assessed for neutralization sensitivity using a set of HIV-2 Env-directed monoclonal antibodies and co-receptor indicator cell lines. Neutralization sensitivity of the isolates was analysed in relation target cell co-receptor expression, in addition to amino acid motifs and predicted structures of Env regions. Results showed that HIV-2 isolates were more resistant to neutralizing antibodies when entering target cells via the alternative co-receptor GPR15, as compared to CCR5. A similar pattern was noted for isolates using the alternative co-receptor CXCR6. Sensitivity to neutralizing antibodies appeared also to be linked to specific Env motifs in V1/V2 and C3 regions. Our findings suggest that HIV-2 sensitivity to neutralization depends both on which co-receptor is used for cell entry and on specific Env motifs. This study highlights the multifactorial mechanisms behind HIV-2 neutralization sensitivity.
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- author
- Szojka, Zsófia Ilona LU ; Karlson, Sara LU ; Johansson, Emil LU ; Şahin, Gülşen Özkaya LU and Jansson, Marianne LU
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Env motifs, HIV-2, neutralization sensitivity, target cell co-receptors
- in
- International Journal of Molecular Sciences
- volume
- 23
- issue
- 9
- article number
- 4766
- publisher
- MDPI AG
- external identifiers
-
- scopus:85128738879
- pmid:35563157
- ISSN
- 1661-6596
- DOI
- 10.3390/ijms23094766
- language
- English
- LU publication?
- yes
- id
- 7656105e-92d9-478c-b2ba-7192eda9cb53
- date added to LUP
- 2022-06-30 14:43:17
- date last changed
- 2024-07-11 17:11:50
@article{7656105e-92d9-478c-b2ba-7192eda9cb53, abstract = {{<p>HIV-2, compared to HIV-1, elicits potent and broadly neutralizing antibodies, and uses a broad range of co-receptors. However, both sensitivity to neutralization and breadth of co-receptor use varies between HIV-2 isolates, and the molecular background is still not fully understood. Thus, in the current study, we have deciphered relationships between HIV-2 neutralization sensitivity, co-receptor use and viral envelope glycoprotein (Env) molecular motifs. A panel of primary HIV-2 isolates, with predefined use of co-receptors, was assessed for neutralization sensitivity using a set of HIV-2 Env-directed monoclonal antibodies and co-receptor indicator cell lines. Neutralization sensitivity of the isolates was analysed in relation target cell co-receptor expression, in addition to amino acid motifs and predicted structures of Env regions. Results showed that HIV-2 isolates were more resistant to neutralizing antibodies when entering target cells via the alternative co-receptor GPR15, as compared to CCR5. A similar pattern was noted for isolates using the alternative co-receptor CXCR6. Sensitivity to neutralizing antibodies appeared also to be linked to specific Env motifs in V1/V2 and C3 regions. Our findings suggest that HIV-2 sensitivity to neutralization depends both on which co-receptor is used for cell entry and on specific Env motifs. This study highlights the multifactorial mechanisms behind HIV-2 neutralization sensitivity.</p>}}, author = {{Szojka, Zsófia Ilona and Karlson, Sara and Johansson, Emil and Şahin, Gülşen Özkaya and Jansson, Marianne}}, issn = {{1661-6596}}, keywords = {{Env motifs; HIV-2; neutralization sensitivity; target cell co-receptors}}, language = {{eng}}, number = {{9}}, publisher = {{MDPI AG}}, series = {{International Journal of Molecular Sciences}}, title = {{HIV-2 Neutralization Sensitivity in Relation to Co-Receptor Entry Pathways and Env Motifs}}, url = {{http://dx.doi.org/10.3390/ijms23094766}}, doi = {{10.3390/ijms23094766}}, volume = {{23}}, year = {{2022}}, }