Studies on the interaction between hyaluronan and a rat colon cancer cell line
(1998) In Glycoconjugate Journal 15(2). p.75-169- Abstract
Binding studies with 125I-Tyr labelled hyaluronan (HA) on a cultured rat colon cancer cell line were performed to characterize the association of HA to tumour cells in vitro. Results show a specific and saturable binding (Kd=1.36 nM) which indicates the presence of an HA binding receptor on the tumour cells. There is a specific constant increase of cell-associated HA over time, which indicates that HA is specifically taken up by the cells through endocytosis. The binding of 125I-Tyr labelled HA was more effectively inhibited by unlabelled HA of high MW in relation to low MW species of the polysaccharide indicating that the receptor binds HA of high MW with greater affinity than low MW species. In competition experiments, the HA-binding... (More)
Binding studies with 125I-Tyr labelled hyaluronan (HA) on a cultured rat colon cancer cell line were performed to characterize the association of HA to tumour cells in vitro. Results show a specific and saturable binding (Kd=1.36 nM) which indicates the presence of an HA binding receptor on the tumour cells. There is a specific constant increase of cell-associated HA over time, which indicates that HA is specifically taken up by the cells through endocytosis. The binding of 125I-Tyr labelled HA was more effectively inhibited by unlabelled HA of high MW in relation to low MW species of the polysaccharide indicating that the receptor binds HA of high MW with greater affinity than low MW species. In competition experiments, the HA-binding could not be inhibited by other polysaccharides such as chondroitin sulphate and heparin. Nor could ligands for scavenger receptors and antibodies directed towards ICAM-1, CD 44 and RHAMM (Receptor for HA Mediated Motility) significantly inhibit the association of HA to tumour cells.
(Less)
- author
- Samuelsson, C LU and Gustafson, S
- publishing date
- 1998-02
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Antibodies/pharmacology, Chondroitin Sulfates/pharmacology, Colonic Neoplasms/drug therapy, Dextran Sulfate/pharmacology, Endothelium/cytology, Extracellular Matrix Proteins/immunology, Hyaluronan Receptors/immunology, Hyaluronic Acid/metabolism, Intercellular Adhesion Molecule-1/immunology, Liver/cytology, Mice, Rats, Rats, Wistar, Tumor Cells, Cultured
- in
- Glycoconjugate Journal
- volume
- 15
- issue
- 2
- pages
- 75 - 169
- publisher
- Springer
- external identifiers
-
- scopus:0031863536
- pmid:9557877
- ISSN
- 0282-0080
- DOI
- 10.1023/A:1006920323095
- language
- English
- LU publication?
- no
- id
- 76c34967-90c4-48e4-a00a-39f88bca12da
- date added to LUP
- 2019-06-05 16:20:31
- date last changed
- 2024-02-15 11:25:04
@article{76c34967-90c4-48e4-a00a-39f88bca12da, abstract = {{<p>Binding studies with 125I-Tyr labelled hyaluronan (HA) on a cultured rat colon cancer cell line were performed to characterize the association of HA to tumour cells in vitro. Results show a specific and saturable binding (Kd=1.36 nM) which indicates the presence of an HA binding receptor on the tumour cells. There is a specific constant increase of cell-associated HA over time, which indicates that HA is specifically taken up by the cells through endocytosis. The binding of 125I-Tyr labelled HA was more effectively inhibited by unlabelled HA of high MW in relation to low MW species of the polysaccharide indicating that the receptor binds HA of high MW with greater affinity than low MW species. In competition experiments, the HA-binding could not be inhibited by other polysaccharides such as chondroitin sulphate and heparin. Nor could ligands for scavenger receptors and antibodies directed towards ICAM-1, CD 44 and RHAMM (Receptor for HA Mediated Motility) significantly inhibit the association of HA to tumour cells.</p>}}, author = {{Samuelsson, C and Gustafson, S}}, issn = {{0282-0080}}, keywords = {{Animals; Antibodies/pharmacology; Chondroitin Sulfates/pharmacology; Colonic Neoplasms/drug therapy; Dextran Sulfate/pharmacology; Endothelium/cytology; Extracellular Matrix Proteins/immunology; Hyaluronan Receptors/immunology; Hyaluronic Acid/metabolism; Intercellular Adhesion Molecule-1/immunology; Liver/cytology; Mice; Rats; Rats, Wistar; Tumor Cells, Cultured}}, language = {{eng}}, number = {{2}}, pages = {{75--169}}, publisher = {{Springer}}, series = {{Glycoconjugate Journal}}, title = {{Studies on the interaction between hyaluronan and a rat colon cancer cell line}}, url = {{http://dx.doi.org/10.1023/A:1006920323095}}, doi = {{10.1023/A:1006920323095}}, volume = {{15}}, year = {{1998}}, }