A validated LC-MS/MS method for the quantification of piperacillin/tazobactam on dried blood spot
(2014) In Bioanalysis 6(21). p.2795-2802- Abstract
- Background Therapeutic drug monitoring (TDM) of antibiotics in children is of fundamental importance for effective patient management. The use of dried blood spot (DBS) offers a number of advantages over conventional blood collection. The aim of this study is to develop and validate a method to measure piperacillin/tazobactam in DBS. Results The analysis was performed by using LC-MS/MS operating in multiple reaction monitoring mode. The method has been validated by applying EMA guidelines and its suitability for TDM was evaluated by using samples from low birth weight neonates. Conclusion This paper describes a fast and cost-effective micromethod for the simultaneous determination of piperacillin/tazobactam levels on dried blood spot that... (More)
- Background Therapeutic drug monitoring (TDM) of antibiotics in children is of fundamental importance for effective patient management. The use of dried blood spot (DBS) offers a number of advantages over conventional blood collection. The aim of this study is to develop and validate a method to measure piperacillin/tazobactam in DBS. Results The analysis was performed by using LC-MS/MS operating in multiple reaction monitoring mode. The method has been validated by applying EMA guidelines and its suitability for TDM was evaluated by using samples from low birth weight neonates. Conclusion This paper describes a fast and cost-effective micromethod for the simultaneous determination of piperacillin/tazobactam levels on dried blood spot that is suitable for TDM in children. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7855465
- author
- Barco, Sebastiano ; Risso, Francesco Maria ; Bruschettini, Matteo LU ; Bandettini, Roberto ; Ramenghi, Luca A. ; Tripodi, Gino ; Castagnola, Elio and Cangemi, Giuliana
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Bioanalysis
- volume
- 6
- issue
- 21
- pages
- 2795 - 2802
- publisher
- Future Science
- external identifiers
-
- wos:000346699600002
- scopus:84918496205
- ISSN
- 1757-6180
- DOI
- 10.4155/BIO.14.205
- language
- English
- LU publication?
- no
- id
- 0e4f2040-3a65-410d-b4bb-95ce30a7e813 (old id 7855465)
- date added to LUP
- 2016-04-01 10:53:01
- date last changed
- 2022-01-26 03:28:56
@article{0e4f2040-3a65-410d-b4bb-95ce30a7e813, abstract = {{Background Therapeutic drug monitoring (TDM) of antibiotics in children is of fundamental importance for effective patient management. The use of dried blood spot (DBS) offers a number of advantages over conventional blood collection. The aim of this study is to develop and validate a method to measure piperacillin/tazobactam in DBS. Results The analysis was performed by using LC-MS/MS operating in multiple reaction monitoring mode. The method has been validated by applying EMA guidelines and its suitability for TDM was evaluated by using samples from low birth weight neonates. Conclusion This paper describes a fast and cost-effective micromethod for the simultaneous determination of piperacillin/tazobactam levels on dried blood spot that is suitable for TDM in children.}}, author = {{Barco, Sebastiano and Risso, Francesco Maria and Bruschettini, Matteo and Bandettini, Roberto and Ramenghi, Luca A. and Tripodi, Gino and Castagnola, Elio and Cangemi, Giuliana}}, issn = {{1757-6180}}, language = {{eng}}, number = {{21}}, pages = {{2795--2802}}, publisher = {{Future Science}}, series = {{Bioanalysis}}, title = {{A validated LC-MS/MS method for the quantification of piperacillin/tazobactam on dried blood spot}}, url = {{http://dx.doi.org/10.4155/BIO.14.205}}, doi = {{10.4155/BIO.14.205}}, volume = {{6}}, year = {{2014}}, }