BL1391 : an established cell line from a human malignant peripheral nerve sheath tumor with unique genomic features
(2021) In Human Cell 34(1). p.238-245- Abstract
Malignant peripheral nerve sheath tumors (MPNSTs) are aggressive tumors, accounting for around 5% of all soft tissue sarcomas. A better understanding of the pathogenesis of these tumors and the development of effective treatments are needed. In this context, established tumor cell lines can be very informative, as they may be used for in-depth molecular analyses and improvement of treatment strategies. Here, we present the genomic and transcriptomic profiling analysis of a MPNST cell line (BL1391) that was spontaneously established in our laboratory from a primary tumor that had not been exposed to genotoxic treatment. This cell line shows peculiar genetic features, such as a large marker chromosome composed of high-copy number... (More)
Malignant peripheral nerve sheath tumors (MPNSTs) are aggressive tumors, accounting for around 5% of all soft tissue sarcomas. A better understanding of the pathogenesis of these tumors and the development of effective treatments are needed. In this context, established tumor cell lines can be very informative, as they may be used for in-depth molecular analyses and improvement of treatment strategies. Here, we present the genomic and transcriptomic profiling analysis of a MPNST cell line (BL1391) that was spontaneously established in our laboratory from a primary tumor that had not been exposed to genotoxic treatment. This cell line shows peculiar genetic features, such as a large marker chromosome composed of high-copy number amplifications of regions from chromosomes 1 and 11 with an embedded neocentromere. Moreover, the transcriptome profiling revealed the presence of several fusion transcripts involving the CACHD1, TNMA4, MDM4, and YAP1 genes, all of which map to the amplified regions of the marker. BL1391 could be a useful tool to study genomic amplifications and neocentromere seeding in MPNSTs and to develop new therapeutic strategies.
(Less)
- author
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Amplification, BL1391, Fusion transcript, MPNST, Neocentromere
- in
- Human Cell
- volume
- 34
- issue
- 1
- pages
- 238 - 245
- publisher
- Springer
- external identifiers
-
- scopus:85089992662
- pmid:32856169
- ISSN
- 0914-7470
- DOI
- 10.1007/s13577-020-00418-7
- language
- English
- LU publication?
- yes
- id
- 7f36a752-6eff-4d0c-ada8-8e42637fbebc
- date added to LUP
- 2020-09-25 14:46:05
- date last changed
- 2024-08-22 04:09:24
@article{7f36a752-6eff-4d0c-ada8-8e42637fbebc, abstract = {{<p>Malignant peripheral nerve sheath tumors (MPNSTs) are aggressive tumors, accounting for around 5% of all soft tissue sarcomas. A better understanding of the pathogenesis of these tumors and the development of effective treatments are needed. In this context, established tumor cell lines can be very informative, as they may be used for in-depth molecular analyses and improvement of treatment strategies. Here, we present the genomic and transcriptomic profiling analysis of a MPNST cell line (BL1391) that was spontaneously established in our laboratory from a primary tumor that had not been exposed to genotoxic treatment. This cell line shows peculiar genetic features, such as a large marker chromosome composed of high-copy number amplifications of regions from chromosomes 1 and 11 with an embedded neocentromere. Moreover, the transcriptome profiling revealed the presence of several fusion transcripts involving the CACHD1, TNMA4, MDM4, and YAP1 genes, all of which map to the amplified regions of the marker. BL1391 could be a useful tool to study genomic amplifications and neocentromere seeding in MPNSTs and to develop new therapeutic strategies.</p>}}, author = {{Tolomeo, Doron and Agostini, Antonio and Macchia, Gemma and L’Abbate, Alberto and Severgnini, Marco and Cifola, Ingrid and Frassanito, Maria Antonia and Racanelli, Vito and Solimando, Antonio Giovanni and Haglund, Felix and Mertens, Fredrik and Storlazzi, Clelia Tiziana}}, issn = {{0914-7470}}, keywords = {{Amplification; BL1391; Fusion transcript; MPNST; Neocentromere}}, language = {{eng}}, number = {{1}}, pages = {{238--245}}, publisher = {{Springer}}, series = {{Human Cell}}, title = {{BL1391 : an established cell line from a human malignant peripheral nerve sheath tumor with unique genomic features}}, url = {{http://dx.doi.org/10.1007/s13577-020-00418-7}}, doi = {{10.1007/s13577-020-00418-7}}, volume = {{34}}, year = {{2021}}, }