Screening for plant lectins by latex agglutination tests
(1991) In Phytochemistry 30(12). p.4005-4009- Abstract
- The latex agglutination test has been applied as a detection system for lectins, the method being especially useful in locations where the dependence on blood for hemagglutination tests could be minimised. The binding of various glycoproteins and sugars individually to the latex particles facilitated the agglutination with lectins having varying sugar specificities. The glycoproteins used were ovalbumin, horseradish peroxidase, porcine mucin and fetuin, while N-acetylglucosamine, N-acetylgalactosamine comprised the sugars used for binding to latex. The sensitivity of the latex agglutination tests was comparable with that of hemagglutination tests. Sugar binding specificity of the lectins could also be determined by inhibition of the... (More)
- The latex agglutination test has been applied as a detection system for lectins, the method being especially useful in locations where the dependence on blood for hemagglutination tests could be minimised. The binding of various glycoproteins and sugars individually to the latex particles facilitated the agglutination with lectins having varying sugar specificities. The glycoproteins used were ovalbumin, horseradish peroxidase, porcine mucin and fetuin, while N-acetylglucosamine, N-acetylgalactosamine comprised the sugars used for binding to latex. The sensitivity of the latex agglutination tests was comparable with that of hemagglutination tests. Sugar binding specificity of the lectins could also be determined by inhibition of the agglutination in the presence of corresponding free sugars. The method proved to be useful in screening crude seed extracts for the presence of lectins. (Less)
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- author
- Kaul, Rajni LU ; Read, John and Mattiasson, Bo LU
- organization
- publishing date
- 1991
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Phytochemistry
- volume
- 30
- issue
- 12
- pages
- 4005 - 4009
- publisher
- Elsevier
- external identifiers
-
- scopus:0011085561
- ISSN
- 0031-9422
- language
- English
- LU publication?
- yes
- id
- 8119240b-edd9-475f-80cb-d2b84590cb0e
- date added to LUP
- 2019-06-29 18:34:51
- date last changed
- 2021-04-04 04:31:46
@article{8119240b-edd9-475f-80cb-d2b84590cb0e, abstract = {{The latex agglutination test has been applied as a detection system for lectins, the method being especially useful in locations where the dependence on blood for hemagglutination tests could be minimised. The binding of various glycoproteins and sugars individually to the latex particles facilitated the agglutination with lectins having varying sugar specificities. The glycoproteins used were ovalbumin, horseradish peroxidase, porcine mucin and fetuin, while N-acetylglucosamine, N-acetylgalactosamine comprised the sugars used for binding to latex. The sensitivity of the latex agglutination tests was comparable with that of hemagglutination tests. Sugar binding specificity of the lectins could also be determined by inhibition of the agglutination in the presence of corresponding free sugars. The method proved to be useful in screening crude seed extracts for the presence of lectins.}}, author = {{Kaul, Rajni and Read, John and Mattiasson, Bo}}, issn = {{0031-9422}}, language = {{eng}}, number = {{12}}, pages = {{4005--4009}}, publisher = {{Elsevier}}, series = {{Phytochemistry}}, title = {{Screening for plant lectins by latex agglutination tests}}, volume = {{30}}, year = {{1991}}, }