Three Redundant Synthetases Secure Redox-Active Pigment Production in the Basidiomycete Paxillus involutus.
(2015) In Chemistry and Biology 22(10). p.1325-1334- Abstract
- The symbiotic fungus Paxillus involutus serves a critical role in maintaining forest ecosystems, which are carbon sinks of global importance. P. involutus produces involutin and other 2,5-diarylcyclopentenone pigments that presumably assist in the oxidative degradation of lignocellulose via Fenton chemistry. Their precise biosynthetic pathways, however, remain obscure. Using a combination of biochemical, genetic, and transcriptomic analyses, in addition to stable-isotope labeling with synthetic precursors, we show that atromentin is the key intermediate. Atromentin is made by tridomain synthetases of high similarity: InvA1, InvA2, and InvA5. An inactive atromentin synthetase, InvA3, gained activity after a domain swap that replaced its... (More)
- The symbiotic fungus Paxillus involutus serves a critical role in maintaining forest ecosystems, which are carbon sinks of global importance. P. involutus produces involutin and other 2,5-diarylcyclopentenone pigments that presumably assist in the oxidative degradation of lignocellulose via Fenton chemistry. Their precise biosynthetic pathways, however, remain obscure. Using a combination of biochemical, genetic, and transcriptomic analyses, in addition to stable-isotope labeling with synthetic precursors, we show that atromentin is the key intermediate. Atromentin is made by tridomain synthetases of high similarity: InvA1, InvA2, and InvA5. An inactive atromentin synthetase, InvA3, gained activity after a domain swap that replaced its native thioesterase domain with that of InvA5. The found degree of multiplex biosynthetic capacity is unprecedented with fungi, and highlights the great importance of the metabolite for the producer. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8148519
- author
- Braesel, Jana ; Götze, Sebastian ; Shah, Firoz LU ; Heine, Daniel ; Tauber, James ; Hertweck, Christian ; Tunlid, Anders LU ; Stallforth, Pierre and Hoffmeister, Dirk
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Chemistry and Biology
- volume
- 22
- issue
- 10
- pages
- 1325 - 1334
- publisher
- Cell Press
- external identifiers
-
- pmid:26496685
- wos:000366140900005
- scopus:84950252285
- pmid:26496685
- ISSN
- 1879-1301
- DOI
- 10.1016/j.chembiol.2015.08.016
- project
- MICCS - Molecular Interactions Controlling soil Carbon Sequestration
- language
- English
- LU publication?
- yes
- id
- 49d345d6-5921-4f42-b6be-1d7119941e10 (old id 8148519)
- date added to LUP
- 2016-04-01 11:10:44
- date last changed
- 2024-05-07 04:38:47
@article{49d345d6-5921-4f42-b6be-1d7119941e10, abstract = {{The symbiotic fungus Paxillus involutus serves a critical role in maintaining forest ecosystems, which are carbon sinks of global importance. P. involutus produces involutin and other 2,5-diarylcyclopentenone pigments that presumably assist in the oxidative degradation of lignocellulose via Fenton chemistry. Their precise biosynthetic pathways, however, remain obscure. Using a combination of biochemical, genetic, and transcriptomic analyses, in addition to stable-isotope labeling with synthetic precursors, we show that atromentin is the key intermediate. Atromentin is made by tridomain synthetases of high similarity: InvA1, InvA2, and InvA5. An inactive atromentin synthetase, InvA3, gained activity after a domain swap that replaced its native thioesterase domain with that of InvA5. The found degree of multiplex biosynthetic capacity is unprecedented with fungi, and highlights the great importance of the metabolite for the producer.}}, author = {{Braesel, Jana and Götze, Sebastian and Shah, Firoz and Heine, Daniel and Tauber, James and Hertweck, Christian and Tunlid, Anders and Stallforth, Pierre and Hoffmeister, Dirk}}, issn = {{1879-1301}}, language = {{eng}}, number = {{10}}, pages = {{1325--1334}}, publisher = {{Cell Press}}, series = {{Chemistry and Biology}}, title = {{Three Redundant Synthetases Secure Redox-Active Pigment Production in the Basidiomycete Paxillus involutus.}}, url = {{http://dx.doi.org/10.1016/j.chembiol.2015.08.016}}, doi = {{10.1016/j.chembiol.2015.08.016}}, volume = {{22}}, year = {{2015}}, }