The association between plasma 25-hydroxyvitamin D and subgroups in age-related macular degeneration : a cross-sectional study
(2013) In PLoS ONE 8(7). p.1-9- Abstract
OBJECTIVES: To evaluate potential differences in plasma 25-hydroxyvitamin in subtypes of age-related macular degeneration (AMD), and in patients in Clinical Age-Related Maculopathy Staging (CARMS) group 5 with or without subretinal fibrosis.
METHODS: This single-center cross-sectional study included 178 participants during a period of 20 months. Ninety-five patients belonged to CARMS 5; twelve belonged to CARMS 4; twenty-two belonged to CARMS 2 or 3; and 49 individuals did not have AMD (CARMS 1). Following a structured interview, a detailed bilateral retinal examination was performed and participants were allocated to their respective subgroups in accordance with the Clinical Age-Related Maculopathy Staging system. Plasma... (More)
OBJECTIVES: To evaluate potential differences in plasma 25-hydroxyvitamin in subtypes of age-related macular degeneration (AMD), and in patients in Clinical Age-Related Maculopathy Staging (CARMS) group 5 with or without subretinal fibrosis.
METHODS: This single-center cross-sectional study included 178 participants during a period of 20 months. Ninety-five patients belonged to CARMS 5; twelve belonged to CARMS 4; twenty-two belonged to CARMS 2 or 3; and 49 individuals did not have AMD (CARMS 1). Following a structured interview, a detailed bilateral retinal examination was performed and participants were allocated to their respective subgroups in accordance with the Clinical Age-Related Maculopathy Staging system. Plasma 25-hydroxyvitamin D2 and D3 were analyzed using liquid chromatography-tandem mass spectrometry. Genomic DNA was extracted from leukocytes and genotyped for single nucleotide polymorphisms (SNPs) in the vitamin D metabolism. Differences in plasma 25-hydroxyvitamin D were determined in the subgroups as well as between patients in CARMS 5 with or without subretinal fibrosis.
RESULTS: Plasma 25-hydroxyvitamin D was comparable in patients across CARMS groups 1 to 5 (p = 0.83). In CARMS 5, the presence of subretinal fibrosis was associated with significantly lower concentrations of 25-hydroxyvitamin D as compared to the absence of subretinal fibrosis (47.2 versus 75.6 nmol/L, p<0.001). Patients in CARMS 5 with subretinal fibrosis were more likely to have insufficient levels of 25-hydroxyvitamin D compared to patients without subretinal fibrosis (p = 0.006). No association was found between the SNPs rs10877012, rs2228570, rs4588, or rs7041 and AMD subgroups or plasma 25-hydroxyvitamin levels.
CONCLUSIONS: This study suggests that the presence of subretinal fibrosis in patients belonging to CARMS 5 may be associated with a poor vitamin D status. Our observations warrant further investigation into the role of vitamin D in the development of subretinal fibrosis.
(Less)
- author
- Singh, Amardeep LU ; Falk, Mads Krüger ; Subhi, Yousif and Sørensen, Torben Lykke
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aged, Aged, 80 and over, Cross-Sectional Studies, Female, Fibrosis, Humans, Macular Degeneration/blood, Male, Polymorphism, Single Nucleotide, Retina/pathology, Risk Factors, Seasons, Vitamin D/analogs & derivatives
- in
- PLoS ONE
- volume
- 8
- issue
- 7
- article number
- e70948
- pages
- 1 - 9
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- scopus:84880763661
- pmid:23923033
- ISSN
- 1932-6203
- DOI
- 10.1371/journal.pone.0070948
- language
- English
- LU publication?
- no
- id
- 822b9417-25f1-4f77-a882-fb3174d8a6c8
- date added to LUP
- 2019-05-21 10:53:42
- date last changed
- 2024-05-28 11:32:11
@article{822b9417-25f1-4f77-a882-fb3174d8a6c8, abstract = {{<p>OBJECTIVES: To evaluate potential differences in plasma 25-hydroxyvitamin in subtypes of age-related macular degeneration (AMD), and in patients in Clinical Age-Related Maculopathy Staging (CARMS) group 5 with or without subretinal fibrosis.</p><p>METHODS: This single-center cross-sectional study included 178 participants during a period of 20 months. Ninety-five patients belonged to CARMS 5; twelve belonged to CARMS 4; twenty-two belonged to CARMS 2 or 3; and 49 individuals did not have AMD (CARMS 1). Following a structured interview, a detailed bilateral retinal examination was performed and participants were allocated to their respective subgroups in accordance with the Clinical Age-Related Maculopathy Staging system. Plasma 25-hydroxyvitamin D2 and D3 were analyzed using liquid chromatography-tandem mass spectrometry. Genomic DNA was extracted from leukocytes and genotyped for single nucleotide polymorphisms (SNPs) in the vitamin D metabolism. Differences in plasma 25-hydroxyvitamin D were determined in the subgroups as well as between patients in CARMS 5 with or without subretinal fibrosis.</p><p>RESULTS: Plasma 25-hydroxyvitamin D was comparable in patients across CARMS groups 1 to 5 (p = 0.83). In CARMS 5, the presence of subretinal fibrosis was associated with significantly lower concentrations of 25-hydroxyvitamin D as compared to the absence of subretinal fibrosis (47.2 versus 75.6 nmol/L, p<0.001). Patients in CARMS 5 with subretinal fibrosis were more likely to have insufficient levels of 25-hydroxyvitamin D compared to patients without subretinal fibrosis (p = 0.006). No association was found between the SNPs rs10877012, rs2228570, rs4588, or rs7041 and AMD subgroups or plasma 25-hydroxyvitamin levels.</p><p>CONCLUSIONS: This study suggests that the presence of subretinal fibrosis in patients belonging to CARMS 5 may be associated with a poor vitamin D status. Our observations warrant further investigation into the role of vitamin D in the development of subretinal fibrosis.</p>}}, author = {{Singh, Amardeep and Falk, Mads Krüger and Subhi, Yousif and Sørensen, Torben Lykke}}, issn = {{1932-6203}}, keywords = {{Aged; Aged, 80 and over; Cross-Sectional Studies; Female; Fibrosis; Humans; Macular Degeneration/blood; Male; Polymorphism, Single Nucleotide; Retina/pathology; Risk Factors; Seasons; Vitamin D/analogs & derivatives}}, language = {{eng}}, number = {{7}}, pages = {{1--9}}, publisher = {{Public Library of Science (PLoS)}}, series = {{PLoS ONE}}, title = {{The association between plasma 25-hydroxyvitamin D and subgroups in age-related macular degeneration : a cross-sectional study}}, url = {{http://dx.doi.org/10.1371/journal.pone.0070948}}, doi = {{10.1371/journal.pone.0070948}}, volume = {{8}}, year = {{2013}}, }