Unlike VIP, the VIP-related peptides PACAP, helodermin and helospectin suppress electrically evoked contractions of rat vas deferens
(1992) In Regulatory Peptides 40(3). p.331-337- Abstract
We have compared the effects of vasoactive intestinal peptide (VIP) and of the VIP-related peptides pituitary adenylate cyclase activating peptide (PACAP) 1-27 and 1-38, helodermin, helospectin I and helospectin II, on the electrically evoked twitches in the isolated vas deferens of the rat. While VIP was virtually without effect, PACAP 1-38 suppressed the electrically evoked twitches effectively and in a concentration-dependent manner (pIC50 value 7.5). The naturally occurring N-terminal fragment PACAP 1-27 was less effective than PACAP 1-38 (Imax values 37.2% suppression compared to 76.5%) and less potent. The C-terminal fragment PACAP 16-38 was virtually inactive. Also helodermin and helospectin I+II suppressed the electrically... (More)
We have compared the effects of vasoactive intestinal peptide (VIP) and of the VIP-related peptides pituitary adenylate cyclase activating peptide (PACAP) 1-27 and 1-38, helodermin, helospectin I and helospectin II, on the electrically evoked twitches in the isolated vas deferens of the rat. While VIP was virtually without effect, PACAP 1-38 suppressed the electrically evoked twitches effectively and in a concentration-dependent manner (pIC50 value 7.5). The naturally occurring N-terminal fragment PACAP 1-27 was less effective than PACAP 1-38 (Imax values 37.2% suppression compared to 76.5%) and less potent. The C-terminal fragment PACAP 16-38 was virtually inactive. Also helodermin and helospectin I+II suppressed the electrically evoked twitches effectively and in a concentration-dependent manner (pIC50 values 6.9; 7.2; 6.8, respectively). The three peptides produced similar maximum reduction of the twitches (74-80%). The findings suggest that PACAP, helodermin and helospectin suppress the electrically evoked contractions in the rat vas deferens via receptors distinct from VIP receptors.
(Less)
- author
- Grundemar, L LU and Håkanson, R LU
- organization
- publishing date
- 1992-08-13
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Amino Acid Sequence, Animals, Electric Stimulation, In Vitro Techniques, Male, Molecular Sequence Data, Muscle Contraction/drug effects, Muscle, Smooth/drug effects, Neuropeptides/pharmacology, Peptides/pharmacology, Pituitary Adenylate Cyclase-Activating Polypeptide, Rats, Rats, Sprague-Dawley, Vas Deferens/drug effects, Vasoactive Intestinal Peptide/pharmacology
- in
- Regulatory Peptides
- volume
- 40
- issue
- 3
- pages
- 331 - 337
- publisher
- Elsevier
- external identifiers
-
- scopus:0026808923
- pmid:1438978
- ISSN
- 0167-0115
- DOI
- 10.1016/0167-0115(92)90520-5
- language
- English
- LU publication?
- yes
- id
- 84d24b03-427a-4f0d-a358-1cf58278104c
- date added to LUP
- 2019-09-03 14:09:48
- date last changed
- 2024-01-01 18:54:12
@article{84d24b03-427a-4f0d-a358-1cf58278104c, abstract = {{<p>We have compared the effects of vasoactive intestinal peptide (VIP) and of the VIP-related peptides pituitary adenylate cyclase activating peptide (PACAP) 1-27 and 1-38, helodermin, helospectin I and helospectin II, on the electrically evoked twitches in the isolated vas deferens of the rat. While VIP was virtually without effect, PACAP 1-38 suppressed the electrically evoked twitches effectively and in a concentration-dependent manner (pIC50 value 7.5). The naturally occurring N-terminal fragment PACAP 1-27 was less effective than PACAP 1-38 (Imax values 37.2% suppression compared to 76.5%) and less potent. The C-terminal fragment PACAP 16-38 was virtually inactive. Also helodermin and helospectin I+II suppressed the electrically evoked twitches effectively and in a concentration-dependent manner (pIC50 values 6.9; 7.2; 6.8, respectively). The three peptides produced similar maximum reduction of the twitches (74-80%). The findings suggest that PACAP, helodermin and helospectin suppress the electrically evoked contractions in the rat vas deferens via receptors distinct from VIP receptors.</p>}}, author = {{Grundemar, L and Håkanson, R}}, issn = {{0167-0115}}, keywords = {{Amino Acid Sequence; Animals; Electric Stimulation; In Vitro Techniques; Male; Molecular Sequence Data; Muscle Contraction/drug effects; Muscle, Smooth/drug effects; Neuropeptides/pharmacology; Peptides/pharmacology; Pituitary Adenylate Cyclase-Activating Polypeptide; Rats; Rats, Sprague-Dawley; Vas Deferens/drug effects; Vasoactive Intestinal Peptide/pharmacology}}, language = {{eng}}, month = {{08}}, number = {{3}}, pages = {{331--337}}, publisher = {{Elsevier}}, series = {{Regulatory Peptides}}, title = {{Unlike VIP, the VIP-related peptides PACAP, helodermin and helospectin suppress electrically evoked contractions of rat vas deferens}}, url = {{http://dx.doi.org/10.1016/0167-0115(92)90520-5}}, doi = {{10.1016/0167-0115(92)90520-5}}, volume = {{40}}, year = {{1992}}, }