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Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells

Luda, Katarzyna M. LU ; Da Silva, Clement LU ; Ahmadi, Fatemeh LU ; Mowat, Allan Mcl LU ; Ohno, Hiroshi ; Kotarsky, Knut LU and Agace, William W. LU (2022) In Scandinavian Journal of Immunology 96(5). p.1-12
Abstract

The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103+CD11b+ cDC in the murine small intestine (SI). GP2 expression was induced on CD103+CD11b+ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103+CD11b+ cDC (huLang-Cre.gp2fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2... (More)

The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103+CD11b+ cDC in the murine small intestine (SI). GP2 expression was induced on CD103+CD11b+ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103+CD11b+ cDC (huLang-Cre.gp2fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103+CD11b+ cDC is not required for intestinal immune homoeostasis.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cDC2, dendritic cell, homeostasis, intestine, IRF4, retinoic acid, TGFβR1
in
Scandinavian Journal of Immunology
volume
96
issue
5
article number
e13219
pages
1 - 12
publisher
Wiley-Blackwell
external identifiers
  • scopus:85140009640
ISSN
0300-9475
DOI
10.1111/sji.13219
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2022 The Authors. Scandinavian Journal of Immunology published by John Wiley & Sons Ltd on behalf of The Scandinavian Foundation for Immunology.
id
859dfe8c-562a-45e0-9102-99b10ce3e3f4
date added to LUP
2022-11-18 16:10:02
date last changed
2023-04-06 00:41:09
@article{859dfe8c-562a-45e0-9102-99b10ce3e3f4,
  abstract     = {{<p>The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103<sup>+</sup>CD11b<sup>+</sup> cDC in the murine small intestine (SI). GP2 expression was induced on CD103<sup>+</sup>CD11b<sup>+</sup> cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103<sup>+</sup>CD11b<sup>+</sup> cDC (huLang-Cre.gp2<sup>fl/fl</sup> mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103<sup>+</sup>CD11b<sup>+</sup> cDC is not required for intestinal immune homoeostasis.</p>}},
  author       = {{Luda, Katarzyna M. and Da Silva, Clement and Ahmadi, Fatemeh and Mowat, Allan Mcl and Ohno, Hiroshi and Kotarsky, Knut and Agace, William W.}},
  issn         = {{0300-9475}},
  keywords     = {{cDC2; dendritic cell; homeostasis; intestine; IRF4; retinoic acid; TGFβR1}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1--12}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Scandinavian Journal of Immunology}},
  title        = {{Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells}},
  url          = {{http://dx.doi.org/10.1111/sji.13219}},
  doi          = {{10.1111/sji.13219}},
  volume       = {{96}},
  year         = {{2022}},
}