Rabbits were immunised with suspensions of viable, insulin-producing islet cells prepared from collagenase-isolated rat or ob/ob mouse pancreatic islets. Antibodies reactive with the surface of dispersed rat islet cells were present in both the rabbit anti-rat and the rabbit anti -ob/ob mouse islet sera as revealed by indirect immunofluorescence or by a radioligandassay using 125
I-Protein A as a measure of cell bound IgG. In a competition assay the binding of 125
I-Protein A was displaced in a concentration dependent manner by non-radioactive Protein A.... (More)
Rabbits were immunised with suspensions of viable, insulin-producing islet cells prepared from collagenase-isolated rat or ob/ob mouse pancreatic islets. Antibodies reactive with the surface of dispersed rat islet cells were present in both the rabbit anti-rat and the rabbit anti -ob/ob mouse islet sera as revealed by indirect immunofluorescence or by a radioligandassay using 125
I-Protein A as a measure of cell bound IgG. In a competition assay the binding of 125
I-Protein A was displaced in a concentration dependent manner by non-radioactive Protein A. Maximal displacement was found at concentrations of Protein A higher than 0.1 μg. added to 10 5
islet cells. Although not always detected by immunofluorescence there was a several-fold increase above normal rabbit serum of 125
I-Protein A-binding to rat hepatocytes and spleen lymphocytes incubated with the islet cell antisera. Conversely, rabbit antisera against rat spleen lymphocytes or against a rat liver plasma membrane preparation reacted with rat islet cells. The rabbit anti-rat islet cell antiserum was absorbed to both spleen lymphocytes and hepatocytes until there was no binding of 125
I-Protein A to either cell type. Islet specific antibodies were still present since this doubly absorbed antiserum induced cell surface immunofluorescence as well as 125
I-Protein A-binding to rat islet cells. It is concluded that apart from common antigenic determinants immunisation with viable islet cells induces formation of antibodies directed against specific islet cell surface components.
@article{864832c6-42f7-41e3-920d-c7acf1602bba,
abstract = {{<p><br>
Rabbits were immunised with suspensions of viable, insulin-producing islet cells prepared from collagenase-isolated rat or ob/ob mouse pancreatic islets. Antibodies reactive with the surface of dispersed rat islet cells were present in both the rabbit anti-rat and the rabbit anti -ob/ob mouse islet sera as revealed by indirect immunofluorescence or by a radioligandassay using <br>
<sup>125</sup><br>
I-Protein A as a measure of cell bound IgG. In a competition assay the binding of <br>
<sup>125</sup><br>
I-Protein A was displaced in a concentration dependent manner by non-radioactive Protein A. Maximal displacement was found at concentrations of Protein A higher than 0.1 μg. added to 10<br>
<sup>5</sup><br>
islet cells. Although not always detected by immunofluorescence there was a several-fold increase above normal rabbit serum of <br>
<sup>125</sup><br>
I-Protein A-binding to rat hepatocytes and spleen lymphocytes incubated with the islet cell antisera. Conversely, rabbit antisera against rat spleen lymphocytes or against a rat liver plasma membrane preparation reacted with rat islet cells. The rabbit anti-rat islet cell antiserum was absorbed to both spleen lymphocytes and hepatocytes until there was no binding of <br>
<sup>125</sup><br>
I-Protein A to either cell type. Islet specific antibodies were still present since this doubly absorbed antiserum induced cell surface immunofluorescence as well as <br>
<sup>125</sup><br>
I-Protein A-binding to rat islet cells. It is concluded that apart from common antigenic determinants immunisation with viable islet cells induces formation of antibodies directed against specific islet cell surface components.</p>}},
author = {{Lernmark, Å and Kanatsuna, T. and Patzelt, C. and Diakoumis, K. and Carroll, R. and Rubenstein, A. H. and Steiner, D. F.}},
issn = {{0012-186X}},
keywords = {{autoimmunity; cell surface antigens; cell surface immunofluorescence; cell suspensions; diabetes mellitus; islet cell surface antibodies; Pancreatic islet cells; Protein A radioassay}},
language = {{eng}},
month = {{11}},
number = {{5}},
pages = {{445--451}},
publisher = {{Springer}},
series = {{Diabetologia}},
title = {{Antibodies directed against the pancreatic islet cell plasma membrane detection and specificity}},
url = {{http://dx.doi.org/10.1007/BF00281824}},
doi = {{10.1007/BF00281824}},
volume = {{19}},
year = {{1980}},
}