Microsatellite primers in the planktonic diatom Pseudo-nitzschia multistriata (Bacillariophyceae)
(2011) In American Journal of Botany 98(2). p.5-33- Abstract
- Premise of the study: Seven microsatellite loci were characterized for the toxic diatom Pseudo-nitzschia multistriata Takano (Takano) to investigate intraspecific variability and estimate population genetic structure over blooms, seasons, and sexual and vegetative reproduction.
Methods and Results: Selected microsatellites consisted of di- and trinucleotide repeats in the core region, and showed four to twelve alleles per locus in strains of P. multistriata collected in the Gulf of Naples (Italy). Primer pairs were species-specific since they positively amplified against conspecific strains from Portugal and Spain but failed to generate PCR products from the diatoms Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and... (More) - Premise of the study: Seven microsatellite loci were characterized for the toxic diatom Pseudo-nitzschia multistriata Takano (Takano) to investigate intraspecific variability and estimate population genetic structure over blooms, seasons, and sexual and vegetative reproduction.
Methods and Results: Selected microsatellites consisted of di- and trinucleotide repeats in the core region, and showed four to twelve alleles per locus in strains of P. multistriata collected in the Gulf of Naples (Italy). Primer pairs were species-specific since they positively amplified against conspecific strains from Portugal and Spain but failed to generate PCR products from the diatoms Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and Leptocylindrus minimum Gran.
Conclusions: The seven selected microsatellite markers will be useful in studying population dynamics of Pseudo-nitzschia multistriata in space and time. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8771187
- author
- Tesson, Sylvie V.M. LU ; Borra, Marco ; Kooistra, Wiebe and Procaccini, Gabriele
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- microsatellites, mutation accumulation lines, mutation rate., Mendelian inheritance, diatom, Asexual and sexual reproduction
- in
- American Journal of Botany
- volume
- 98
- issue
- 2
- pages
- 5 - 33
- publisher
- Botanical Society of America
- external identifiers
-
- scopus:79851504956
- pmid:21613102
- ISSN
- 0002-9122
- DOI
- 10.3732/ajb.1000430
- language
- English
- LU publication?
- yes
- id
- 9ef7dbcb-5c53-44f7-842d-6d318c7c5c4c (old id 8771187)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21613102
- date added to LUP
- 2016-04-04 09:38:43
- date last changed
- 2024-03-22 09:45:42
@article{9ef7dbcb-5c53-44f7-842d-6d318c7c5c4c,
abstract = {{Premise of the study: Seven microsatellite loci were characterized for the toxic diatom Pseudo-nitzschia multistriata Takano (Takano) to investigate intraspecific variability and estimate population genetic structure over blooms, seasons, and sexual and vegetative reproduction. <br/><br>
Methods and Results: Selected microsatellites consisted of di- and trinucleotide repeats in the core region, and showed four to twelve alleles per locus in strains of P. multistriata collected in the Gulf of Naples (Italy). Primer pairs were species-specific since they positively amplified against conspecific strains from Portugal and Spain but failed to generate PCR products from the diatoms Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and Leptocylindrus minimum Gran. <br/><br>
Conclusions: The seven selected microsatellite markers will be useful in studying population dynamics of Pseudo-nitzschia multistriata in space and time.}},
author = {{Tesson, Sylvie V.M. and Borra, Marco and Kooistra, Wiebe and Procaccini, Gabriele}},
issn = {{0002-9122}},
keywords = {{microsatellites; mutation accumulation lines; mutation rate.; Mendelian inheritance; diatom; Asexual and sexual reproduction}},
language = {{eng}},
number = {{2}},
pages = {{5--33}},
publisher = {{Botanical Society of America}},
series = {{American Journal of Botany}},
title = {{Microsatellite primers in the planktonic diatom Pseudo-nitzschia multistriata (Bacillariophyceae)}},
url = {{http://dx.doi.org/10.3732/ajb.1000430}},
doi = {{10.3732/ajb.1000430}},
volume = {{98}},
year = {{2011}},
}