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Autoantibodies in newly diagnosed diabetic children immunoprecipitate human pancreatic islet cell proteins

Baekkeskov, Steinunn ; Nielsen, Jens Høiriis ; Marner, Birgitte ; Bilde, Torben ; Ludvigsson, Johnny and Lernmark, Ake LU orcid (1982) In Nature 298(5870). p.167-169
Abstract

Insulin-dependent diabetic (IDD) patients have a high prevalence of circulating autoantibodies against islet of Langerhans cells at the time of diagnosis1-4. Inflammatory cells within the islets5, leukocyte migration inhibition in response to pancreatic antigens6 and an association with certain HLA-D/DR histocompatibility antigens 7,8, have also been observed. It seems that the autoantibodies may be pathogenically relevant as they react primarily with β-cells9, but the specific target antigen(s) have yet to be identified. In the present study we determined whether sera from insulin-dependent diabetic children are able to immunoprecipitate proteins from detergent lysates of human... (More)

Insulin-dependent diabetic (IDD) patients have a high prevalence of circulating autoantibodies against islet of Langerhans cells at the time of diagnosis1-4. Inflammatory cells within the islets5, leukocyte migration inhibition in response to pancreatic antigens6 and an association with certain HLA-D/DR histocompatibility antigens 7,8, have also been observed. It seems that the autoantibodies may be pathogenically relevant as they react primarily with β-cells9, but the specific target antigen(s) have yet to be identified. In the present study we determined whether sera from insulin-dependent diabetic children are able to immunoprecipitate proteins from detergent lysates of human islet cells. We report that sera from 8 out of 10 newly diagnosed diabetic children consistently immunoprecipitate a protein having a molecular weight (M r) of ∼64,000 (64K). An additional protein (38K) was precipitated from islet cells obtained from a HLA-DR3-positive donor. Neither of the proteins was precipitated by non-diabetic sera nor detected in immunoprecipitates from human lymphocyte lysates. It is suggested that the 64K and/or 38K protein components may represent cell-specific target antigens in insulin-dependent diabetes.

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author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Nature
volume
298
issue
5870
pages
3 pages
publisher
Nature Publishing Group
external identifiers
  • pmid:7045690
  • scopus:0019959221
ISSN
0028-0836
DOI
10.1038/298167a0
language
English
LU publication?
no
id
877bb43c-b628-4848-a290-6d97fcc3e64e
date added to LUP
2019-09-16 15:32:53
date last changed
2024-03-13 08:14:06
@article{877bb43c-b628-4848-a290-6d97fcc3e64e,
  abstract     = {{<p>Insulin-dependent diabetic (IDD) patients have a high prevalence of circulating autoantibodies against islet of Langerhans cells at the time of diagnosis<sup>1-4</sup>. Inflammatory cells within the islets<sup>5</sup>, leukocyte migration inhibition in response to pancreatic antigens<sup>6</sup> and an association with certain HLA-D/DR histocompatibility antigens <sup>7,8</sup>, have also been observed. It seems that the autoantibodies may be pathogenically relevant as they react primarily with β-cells<sup>9</sup>, but the specific target antigen(s) have yet to be identified. In the present study we determined whether sera from insulin-dependent diabetic children are able to immunoprecipitate proteins from detergent lysates of human islet cells. We report that sera from 8 out of 10 newly diagnosed diabetic children consistently immunoprecipitate a protein having a molecular weight (M <sub>r</sub>) of ∼64,000 (64K). An additional protein (38K) was precipitated from islet cells obtained from a HLA-DR3-positive donor. Neither of the proteins was precipitated by non-diabetic sera nor detected in immunoprecipitates from human lymphocyte lysates. It is suggested that the 64K and/or 38K protein components may represent cell-specific target antigens in insulin-dependent diabetes.</p>}},
  author       = {{Baekkeskov, Steinunn and Nielsen, Jens Høiriis and Marner, Birgitte and Bilde, Torben and Ludvigsson, Johnny and Lernmark, Ake}},
  issn         = {{0028-0836}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{5870}},
  pages        = {{167--169}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature}},
  title        = {{Autoantibodies in newly diagnosed diabetic children immunoprecipitate human pancreatic islet cell proteins}},
  url          = {{http://dx.doi.org/10.1038/298167a0}},
  doi          = {{10.1038/298167a0}},
  volume       = {{298}},
  year         = {{1982}},
}