Magnetic aqueous two-phase separation: A new technique to increase rate of phase-separation, using dextran-ferrofluid or larger iron oxide particles
(1987) In Analytical Biochemistry 167(2). p.331-339- Abstract
- A new technique to speed up the phase separation of aqueous two-phase systems is described. The technique is based on the addition of magnetically susceptible additives (ferrofluids or iron oxide particles). In a magnetic field such additives will induce a faster phase separation. In one approach, dextran-stabilized ferrofluid was added to an aqueous two-phase system containing polyethylene glycol and dextran. The ferrofluid was totally partitioned to the dextran phase. After mixing of the two-phase system, it was possible to reduce the separation time by a factor of 35 by applying a magnetic field to the system. Another approach involved the use of 1-μm iron oxide particles instead of ferrofluid. In this case also, the phase-separation... (More)
- A new technique to speed up the phase separation of aqueous two-phase systems is described. The technique is based on the addition of magnetically susceptible additives (ferrofluids or iron oxide particles). In a magnetic field such additives will induce a faster phase separation. In one approach, dextran-stabilized ferrofluid was added to an aqueous two-phase system containing polyethylene glycol and dextran. The ferrofluid was totally partitioned to the dextran phase. After mixing of the two-phase system, it was possible to reduce the separation time by a factor of 35 by applying a magnetic field to the system. Another approach involved the use of 1-μm iron oxide particles instead of ferrofluid. In this case also, the phase-separation time was reduced, by a factor of about 70, when the system was placed in a magnetic field. The addition of ferrofluid and/or iron oxide particles was shown to have no influence on enzyme partitioning or on enzyme activity. The partitioning of chloroplasts, on the other hand, was influenced unless the ferrofluid used had been treated with epoxysilane. A column system comprising 15 magnetic separation stages was constructed and was used for semicontinuous separation of enzyme mixtures. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8a129364-972d-45c5-93f4-499b980247b9
- author
- Wikström, Per ; Flygare, Susanne ; Gröndalen, Anne and Larsson, Per-Olof LU
- organization
- publishing date
- 1987
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- aqueous two-phase separation, magnetic separation, ferrofluid, iron oxide particles
- in
- Analytical Biochemistry
- volume
- 167
- issue
- 2
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0023489952
- ISSN
- 0003-2697
- DOI
- 10.1016/0003-2697(87)90173-4
- language
- English
- LU publication?
- yes
- id
- 8a129364-972d-45c5-93f4-499b980247b9
- date added to LUP
- 2024-06-18 14:48:24
- date last changed
- 2024-09-06 12:52:38
@article{8a129364-972d-45c5-93f4-499b980247b9,
abstract = {{A new technique to speed up the phase separation of aqueous two-phase systems is described. The technique is based on the addition of magnetically susceptible additives (ferrofluids or iron oxide particles). In a magnetic field such additives will induce a faster phase separation. In one approach, dextran-stabilized ferrofluid was added to an aqueous two-phase system containing polyethylene glycol and dextran. The ferrofluid was totally partitioned to the dextran phase. After mixing of the two-phase system, it was possible to reduce the separation time by a factor of 35 by applying a magnetic field to the system. Another approach involved the use of 1-μm iron oxide particles instead of ferrofluid. In this case also, the phase-separation time was reduced, by a factor of about 70, when the system was placed in a magnetic field. The addition of ferrofluid and/or iron oxide particles was shown to have no influence on enzyme partitioning or on enzyme activity. The partitioning of chloroplasts, on the other hand, was influenced unless the ferrofluid used had been treated with epoxysilane. A column system comprising 15 magnetic separation stages was constructed and was used for semicontinuous separation of enzyme mixtures.}},
author = {{Wikström, Per and Flygare, Susanne and Gröndalen, Anne and Larsson, Per-Olof}},
issn = {{0003-2697}},
keywords = {{aqueous two-phase separation; magnetic separation; ferrofluid; iron oxide particles}},
language = {{eng}},
number = {{2}},
pages = {{331--339}},
publisher = {{Elsevier}},
series = {{Analytical Biochemistry}},
title = {{Magnetic aqueous two-phase separation: A new technique to increase rate of phase-separation, using dextran-ferrofluid or larger iron oxide particles}},
url = {{http://dx.doi.org/10.1016/0003-2697(87)90173-4}},
doi = {{10.1016/0003-2697(87)90173-4}},
volume = {{167}},
year = {{1987}},
}