EFFECT OF KIDNEY STEM CELLS ON BIOCHEMICAL ANALAYSIS AND SMAD2/3 PHOSPHORYLATION IN RATS WITH DIABETIC NEPHROPATHY
(2021) 6th world congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS2021) p.297-297- Abstract
- Introduction: Mesenchymal stem cells (MSCs) were proposed as a critical therapeutic candidate in diabetic nephropathy (DN). Renal stem cells as a source for repairing are controversial. The purpose of the present study was to evaluate the effect of kidney rat stem cells on DN.
Materials and methods: After separation of renal stem cells from rat kidney, the surface Stem cell markers were assessed by flow cytometry analysis. To establish the diabetic nephropathy rat model STZ(60mg/kg) was used. The cells were injected to experimental groups via tail vein (2×106cells/rat). Biochemical and histological parameters were evaluated in order to determining the impact of stem cells on kidney structure. Phosphorylation of Smad2/3 two weeks... (More) - Introduction: Mesenchymal stem cells (MSCs) were proposed as a critical therapeutic candidate in diabetic nephropathy (DN). Renal stem cells as a source for repairing are controversial. The purpose of the present study was to evaluate the effect of kidney rat stem cells on DN.
Materials and methods: After separation of renal stem cells from rat kidney, the surface Stem cell markers were assessed by flow cytometry analysis. To establish the diabetic nephropathy rat model STZ(60mg/kg) was used. The cells were injected to experimental groups via tail vein (2×106cells/rat). Biochemical and histological parameters were evaluated in order to determining the impact of stem cells on kidney structure. Phosphorylation of Smad2/3 two weeks after induction of early diabetic nephropathy was evaluated by using standard western blotting.
Result: The cells significantly reduced blood nitrogen (BUN), serum creatinine (Scr) and 24 urinary proteins. The phosphorylation of smad2 and smad3 significantly down-regulated. PAS staining showed in the presence of adult kidney stem cells histopathological changes were improved.
Conclusion: Adult kidney stem cells may be a candidate for treatment of early DN to improve the kidney function and regenerating kidney tissues in DN rats.
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Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8bc9698a-c02f-40c8-b8c1-a27fbb4f04c7
- author
- Rafiee, Zeinab LU ; Mansouri, Esrafil and Orazizadeh, Mahmoud
- organization
- publishing date
- 2021
- type
- Contribution to conference
- publication status
- in press
- subject
- keywords
- Stem cell therapy, smad signaling, Diabetic nephropathy, kidney stem cells
- pages
- 297 - 297
- conference name
- 6th world congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS2021)
- conference location
- Maastricht, Netherlands
- conference dates
- 2021-11-15 - 2021-11-19
- language
- English
- LU publication?
- yes
- id
- 8bc9698a-c02f-40c8-b8c1-a27fbb4f04c7
- date added to LUP
- 2021-09-25 11:09:02
- date last changed
- 2021-10-04 09:08:54
@misc{8bc9698a-c02f-40c8-b8c1-a27fbb4f04c7, abstract = {{Introduction: Mesenchymal stem cells (MSCs) were proposed as a critical therapeutic candidate in diabetic nephropathy (DN). Renal stem cells as a source for repairing are controversial. The purpose of the present study was to evaluate the effect of kidney rat stem cells on DN.<br/> Materials and methods: After separation of renal stem cells from rat kidney, the surface Stem cell markers were assessed by flow cytometry analysis. To establish the diabetic nephropathy rat model STZ(60mg/kg) was used. The cells were injected to experimental groups via tail vein (2×106cells/rat). Biochemical and histological parameters were evaluated in order to determining the impact of stem cells on kidney structure. Phosphorylation of Smad2/3 two weeks after induction of early diabetic nephropathy was evaluated by using standard western blotting.<br/>Result: The cells significantly reduced blood nitrogen (BUN), serum creatinine (Scr) and 24 urinary proteins. The phosphorylation of smad2 and smad3 significantly down-regulated. PAS staining showed in the presence of adult kidney stem cells histopathological changes were improved.<br/>Conclusion: Adult kidney stem cells may be a candidate for treatment of early DN to improve the kidney function and regenerating kidney tissues in DN rats.<br/>}}, author = {{Rafiee, Zeinab and Mansouri, Esrafil and Orazizadeh, Mahmoud}}, keywords = {{Stem cell therapy; smad signaling; Diabetic nephropathy; kidney stem cells}}, language = {{eng}}, pages = {{297--297}}, title = {{EFFECT OF KIDNEY STEM CELLS ON BIOCHEMICAL ANALAYSIS AND SMAD2/3 PHOSPHORYLATION IN RATS WITH DIABETIC NEPHROPATHY}}, year = {{2021}}, }