Kinase-independent function of Cyclin E
(2007) In Molecular Cell 25(1). p.127-139- Abstract
E-type cyclins are thought to drive cell-cycle progression by activating cyclin-dependent kinases, primarily CDK2. We previously found that cyclin E-null cells failed to incorporate MCM helicase into DNA prereplication complex during G0 → S phase progression. We now report that a kinase-deficient cyclin E mutant can partially restore MCM loading and S phase entry in cyclin E-null cells. We found that cyclin E is loaded onto chromatin during G0 → S progression. In the absence of cyclin E, CDT1 is normally loaded onto chromatin, whereas MCM is not, indicating that cyclin E acts between CDT1 and MCM loading. We observed a physical association of cyclin E with CDT1 and with MCMs. We propose that cyclin E facilitates... (More)
E-type cyclins are thought to drive cell-cycle progression by activating cyclin-dependent kinases, primarily CDK2. We previously found that cyclin E-null cells failed to incorporate MCM helicase into DNA prereplication complex during G0 → S phase progression. We now report that a kinase-deficient cyclin E mutant can partially restore MCM loading and S phase entry in cyclin E-null cells. We found that cyclin E is loaded onto chromatin during G0 → S progression. In the absence of cyclin E, CDT1 is normally loaded onto chromatin, whereas MCM is not, indicating that cyclin E acts between CDT1 and MCM loading. We observed a physical association of cyclin E with CDT1 and with MCMs. We propose that cyclin E facilitates MCM loading in a kinase-independent fashion, through physical interaction with CDT1 and MCM. Our work indicates that-in addition to their function as CDK activators-E cyclins play kinase-independent functions in cell-cycle progression.
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- author
- Geng, Yan ; Lee, Young Mi ; Welcker, Markus ; Swanger, Jherek ; Zagozdzon, Agnieszka ; Winer, Joel D. ; Roberts, James M. ; Kaldis, Philipp LU ; Clurman, Bruce E. and Sicinski, Piotr
- publishing date
- 2007-01-12
- type
- Contribution to journal
- publication status
- published
- keywords
- CELLCYCLE, SIGNALING
- in
- Molecular Cell
- volume
- 25
- issue
- 1
- pages
- 127 - 139
- publisher
- Cell Press
- external identifiers
-
- scopus:33846018073
- pmid:17218276
- ISSN
- 1097-2765
- DOI
- 10.1016/j.molcel.2006.11.029
- language
- English
- LU publication?
- no
- id
- 986916b7-6050-4c1f-ba87-762fc1441370
- date added to LUP
- 2019-09-18 14:18:42
- date last changed
- 2024-09-19 10:12:32
@article{986916b7-6050-4c1f-ba87-762fc1441370, abstract = {{<p>E-type cyclins are thought to drive cell-cycle progression by activating cyclin-dependent kinases, primarily CDK2. We previously found that cyclin E-null cells failed to incorporate MCM helicase into DNA prereplication complex during G<sub>0</sub> → S phase progression. We now report that a kinase-deficient cyclin E mutant can partially restore MCM loading and S phase entry in cyclin E-null cells. We found that cyclin E is loaded onto chromatin during G<sub>0</sub> → S progression. In the absence of cyclin E, CDT1 is normally loaded onto chromatin, whereas MCM is not, indicating that cyclin E acts between CDT1 and MCM loading. We observed a physical association of cyclin E with CDT1 and with MCMs. We propose that cyclin E facilitates MCM loading in a kinase-independent fashion, through physical interaction with CDT1 and MCM. Our work indicates that-in addition to their function as CDK activators-E cyclins play kinase-independent functions in cell-cycle progression.</p>}}, author = {{Geng, Yan and Lee, Young Mi and Welcker, Markus and Swanger, Jherek and Zagozdzon, Agnieszka and Winer, Joel D. and Roberts, James M. and Kaldis, Philipp and Clurman, Bruce E. and Sicinski, Piotr}}, issn = {{1097-2765}}, keywords = {{CELLCYCLE; SIGNALING}}, language = {{eng}}, month = {{01}}, number = {{1}}, pages = {{127--139}}, publisher = {{Cell Press}}, series = {{Molecular Cell}}, title = {{Kinase-independent function of Cyclin E}}, url = {{http://dx.doi.org/10.1016/j.molcel.2006.11.029}}, doi = {{10.1016/j.molcel.2006.11.029}}, volume = {{25}}, year = {{2007}}, }