Caracterización intratípica de Poliovirus por la técnica de reacción en cadena de la polimerasa
(1998) In Revista cubana de medicina tropical 50(2). p.4-100- Abstract
The polymerase chain reaction techniques was introduced for the intratypic characterization of Poliovirus. Primers were used only to promote the amplification of the Sabin vaccine strains proved by electrophoretic run of the amplified DNA products (Sabin 1-97 pb, Sabin 2-71 pb, Sabin 3-44 pb) and whose specificity was satisfactorily verified. 23 Cuban poliovirus strains isolated and identified at the Laboratory of Enterovirus of the "Pedro Kourí" Tropical Medicine Institute from 1993 to 1994 were studied by this technique. All of them were of the vaccine type. It was observed how the Sabin vaccine poliovirus may be the cause of viral meningoencephalitis as a milder neurological complication. This study provided one more evidence about... (More)
The polymerase chain reaction techniques was introduced for the intratypic characterization of Poliovirus. Primers were used only to promote the amplification of the Sabin vaccine strains proved by electrophoretic run of the amplified DNA products (Sabin 1-97 pb, Sabin 2-71 pb, Sabin 3-44 pb) and whose specificity was satisfactorily verified. 23 Cuban poliovirus strains isolated and identified at the Laboratory of Enterovirus of the "Pedro Kourí" Tropical Medicine Institute from 1993 to 1994 were studied by this technique. All of them were of the vaccine type. It was observed how the Sabin vaccine poliovirus may be the cause of viral meningoencephalitis as a milder neurological complication. This study provided one more evidence about the non circulation of the wild poliovirus in Cuba.
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- author
- Avalos Redón, I ; Más Lago, P J ; Sarmiento Pérez, L R LU ; Palomera Puente, R ; Muné Jiménez, M ; Bello Corredor, M and Pérez Santos, L
- alternative title
- The intratypic characterization of the poliovirus by the polymerase chain reaction technic
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Base Sequence, Humans, Molecular Sequence Data, Oligonucleotide Probes, Poliovirus/classification, Polymerase Chain Reaction/methods, RNA, Viral/genetics
- in
- Revista cubana de medicina tropical
- volume
- 50
- issue
- 2
- pages
- 5 pages
- publisher
- Editorial Ciencias Medicas
- external identifiers
-
- scopus:0032249807
- pmid:10349426
- ISSN
- 0375-0760
- language
- Spanish
- LU publication?
- no
- id
- 99d6a68e-2534-422b-895b-4e3d7f8f8077
- alternative location
- http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375-07601998000200004
- date added to LUP
- 2019-07-08 15:18:47
- date last changed
- 2024-10-02 09:15:32
@article{99d6a68e-2534-422b-895b-4e3d7f8f8077, abstract = {{<p>The polymerase chain reaction techniques was introduced for the intratypic characterization of Poliovirus. Primers were used only to promote the amplification of the Sabin vaccine strains proved by electrophoretic run of the amplified DNA products (Sabin 1-97 pb, Sabin 2-71 pb, Sabin 3-44 pb) and whose specificity was satisfactorily verified. 23 Cuban poliovirus strains isolated and identified at the Laboratory of Enterovirus of the "Pedro Kourí" Tropical Medicine Institute from 1993 to 1994 were studied by this technique. All of them were of the vaccine type. It was observed how the Sabin vaccine poliovirus may be the cause of viral meningoencephalitis as a milder neurological complication. This study provided one more evidence about the non circulation of the wild poliovirus in Cuba.</p>}}, author = {{Avalos Redón, I and Más Lago, P J and Sarmiento Pérez, L R and Palomera Puente, R and Muné Jiménez, M and Bello Corredor, M and Pérez Santos, L}}, issn = {{0375-0760}}, keywords = {{Base Sequence; Humans; Molecular Sequence Data; Oligonucleotide Probes; Poliovirus/classification; Polymerase Chain Reaction/methods; RNA, Viral/genetics}}, language = {{spa}}, number = {{2}}, pages = {{4--100}}, publisher = {{Editorial Ciencias Medicas}}, series = {{Revista cubana de medicina tropical}}, title = {{Caracterización intratípica de Poliovirus por la técnica de reacción en cadena de la polimerasa}}, url = {{http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375-07601998000200004}}, volume = {{50}}, year = {{1998}}, }