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Reactivity of anti-PSA monoclonal antibodies with recombinant human kallikrein-2

Leinonen, J. ; Leinimaa, M. ; Zhang, W. M. ; Piironen, T. ; Pettersson, K. ; Lilja, H. LU orcid ; Dowell, B. and Stenman, U. H. (1999) In Tumor Biology 20(SUPPL. 1). p.35-37
Abstract

Seventy-nine monoclonal antibodies submitted to the ISOBM TD-3 PSA Workshop were tested for their reactivity with recombinant human kallikrein-2 (rhK2). A sandwich immunofluorometric assay using polyclonal antiprostate- specific antigen (PSA) antiserum-coated plates was used to capture rhK2 and subsequently the test antibody. The response of each test antibody was compared with 3 reference antibodies (H50, H117 and 5E4) known to react with hK2. Nine antibodies from the workshop panel failed to react with purified PSA and rhK2 in this assay and were subsequently excluded. From the remaining panel of antibodies, 11/70 showed strong reactivity with rhK2, 9/70 showed weak reactivity with rhK2, while 50/70 antibodies did not react with rhK2... (More)

Seventy-nine monoclonal antibodies submitted to the ISOBM TD-3 PSA Workshop were tested for their reactivity with recombinant human kallikrein-2 (rhK2). A sandwich immunofluorometric assay using polyclonal antiprostate- specific antigen (PSA) antiserum-coated plates was used to capture rhK2 and subsequently the test antibody. The response of each test antibody was compared with 3 reference antibodies (H50, H117 and 5E4) known to react with hK2. Nine antibodies from the workshop panel failed to react with purified PSA and rhK2 in this assay and were subsequently excluded. From the remaining panel of antibodies, 11/70 showed strong reactivity with rhK2, 9/70 showed weak reactivity with rhK2, while 50/70 antibodies did not react with rhK2 in this assay format. All antibodies binding to rhK2 recognized both free and complexed PSA.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Human kallikrein-2, ISOBM, Monoclonal antibodies, Prostate-specific antigen, Recombinant kallikrein, TD-3
in
Tumor Biology
volume
20
issue
SUPPL. 1
pages
35 - 37
publisher
Springer
external identifiers
  • pmid:10628407
  • scopus:0032705502
ISSN
1010-4283
DOI
10.1159/000056528
language
English
LU publication?
yes
id
9e225900-e09f-4451-b9ea-694c73b69afb
date added to LUP
2022-12-06 17:47:23
date last changed
2024-07-21 14:50:26
@article{9e225900-e09f-4451-b9ea-694c73b69afb,
  abstract     = {{<p>Seventy-nine monoclonal antibodies submitted to the ISOBM TD-3 PSA Workshop were tested for their reactivity with recombinant human kallikrein-2 (rhK2). A sandwich immunofluorometric assay using polyclonal antiprostate- specific antigen (PSA) antiserum-coated plates was used to capture rhK2 and subsequently the test antibody. The response of each test antibody was compared with 3 reference antibodies (H50, H117 and 5E4) known to react with hK2. Nine antibodies from the workshop panel failed to react with purified PSA and rhK2 in this assay and were subsequently excluded. From the remaining panel of antibodies, 11/70 showed strong reactivity with rhK2, 9/70 showed weak reactivity with rhK2, while 50/70 antibodies did not react with rhK2 in this assay format. All antibodies binding to rhK2 recognized both free and complexed PSA.</p>}},
  author       = {{Leinonen, J. and Leinimaa, M. and Zhang, W. M. and Piironen, T. and Pettersson, K. and Lilja, H. and Dowell, B. and Stenman, U. H.}},
  issn         = {{1010-4283}},
  keywords     = {{Human kallikrein-2; ISOBM; Monoclonal antibodies; Prostate-specific antigen; Recombinant kallikrein; TD-3}},
  language     = {{eng}},
  number       = {{SUPPL. 1}},
  pages        = {{35--37}},
  publisher    = {{Springer}},
  series       = {{Tumor Biology}},
  title        = {{Reactivity of anti-PSA monoclonal antibodies with recombinant human kallikrein-2}},
  url          = {{http://dx.doi.org/10.1159/000056528}},
  doi          = {{10.1159/000056528}},
  volume       = {{20}},
  year         = {{1999}},
}