Reactivity of anti-PSA monoclonal antibodies with recombinant human kallikrein-2
(1999) In Tumor Biology 20(SUPPL. 1). p.35-37- Abstract
Seventy-nine monoclonal antibodies submitted to the ISOBM TD-3 PSA Workshop were tested for their reactivity with recombinant human kallikrein-2 (rhK2). A sandwich immunofluorometric assay using polyclonal antiprostate- specific antigen (PSA) antiserum-coated plates was used to capture rhK2 and subsequently the test antibody. The response of each test antibody was compared with 3 reference antibodies (H50, H117 and 5E4) known to react with hK2. Nine antibodies from the workshop panel failed to react with purified PSA and rhK2 in this assay and were subsequently excluded. From the remaining panel of antibodies, 11/70 showed strong reactivity with rhK2, 9/70 showed weak reactivity with rhK2, while 50/70 antibodies did not react with rhK2... (More)
Seventy-nine monoclonal antibodies submitted to the ISOBM TD-3 PSA Workshop were tested for their reactivity with recombinant human kallikrein-2 (rhK2). A sandwich immunofluorometric assay using polyclonal antiprostate- specific antigen (PSA) antiserum-coated plates was used to capture rhK2 and subsequently the test antibody. The response of each test antibody was compared with 3 reference antibodies (H50, H117 and 5E4) known to react with hK2. Nine antibodies from the workshop panel failed to react with purified PSA and rhK2 in this assay and were subsequently excluded. From the remaining panel of antibodies, 11/70 showed strong reactivity with rhK2, 9/70 showed weak reactivity with rhK2, while 50/70 antibodies did not react with rhK2 in this assay format. All antibodies binding to rhK2 recognized both free and complexed PSA.
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- author
- Leinonen, J. ; Leinimaa, M. ; Zhang, W. M. ; Piironen, T. ; Pettersson, K. ; Lilja, H. LU ; Dowell, B. and Stenman, U. H.
- organization
- publishing date
- 1999-10
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Human kallikrein-2, ISOBM, Monoclonal antibodies, Prostate-specific antigen, Recombinant kallikrein, TD-3
- in
- Tumor Biology
- volume
- 20
- issue
- SUPPL. 1
- pages
- 35 - 37
- publisher
- Springer
- external identifiers
-
- pmid:10628407
- scopus:0032705502
- ISSN
- 1010-4283
- DOI
- 10.1159/000056528
- language
- English
- LU publication?
- yes
- id
- 9e225900-e09f-4451-b9ea-694c73b69afb
- date added to LUP
- 2022-12-06 17:47:23
- date last changed
- 2024-07-21 14:50:26
@article{9e225900-e09f-4451-b9ea-694c73b69afb, abstract = {{<p>Seventy-nine monoclonal antibodies submitted to the ISOBM TD-3 PSA Workshop were tested for their reactivity with recombinant human kallikrein-2 (rhK2). A sandwich immunofluorometric assay using polyclonal antiprostate- specific antigen (PSA) antiserum-coated plates was used to capture rhK2 and subsequently the test antibody. The response of each test antibody was compared with 3 reference antibodies (H50, H117 and 5E4) known to react with hK2. Nine antibodies from the workshop panel failed to react with purified PSA and rhK2 in this assay and were subsequently excluded. From the remaining panel of antibodies, 11/70 showed strong reactivity with rhK2, 9/70 showed weak reactivity with rhK2, while 50/70 antibodies did not react with rhK2 in this assay format. All antibodies binding to rhK2 recognized both free and complexed PSA.</p>}}, author = {{Leinonen, J. and Leinimaa, M. and Zhang, W. M. and Piironen, T. and Pettersson, K. and Lilja, H. and Dowell, B. and Stenman, U. H.}}, issn = {{1010-4283}}, keywords = {{Human kallikrein-2; ISOBM; Monoclonal antibodies; Prostate-specific antigen; Recombinant kallikrein; TD-3}}, language = {{eng}}, number = {{SUPPL. 1}}, pages = {{35--37}}, publisher = {{Springer}}, series = {{Tumor Biology}}, title = {{Reactivity of anti-PSA monoclonal antibodies with recombinant human kallikrein-2}}, url = {{http://dx.doi.org/10.1159/000056528}}, doi = {{10.1159/000056528}}, volume = {{20}}, year = {{1999}}, }