Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Integral and peripheral proteins of the spinach leaf plasma membrane.

Kjellbom, Per LU ; Larsson, Christer LU ; Rochester, Colin P. and Andersson, Bertil (1989) In Plant Physiology and Biochemistry 27. p.169-174
Abstract
Plasma membrane vesicles of high purity were isolated from spinach (Spinacia oleracea) leaves by aqueous polymer 2-phase partition and analyzed with respect to their protein organization. This was done by Triton X-114 fractionation which separates integral, hydrophobic, membrane-spanning proteins from peripheral, hydrophilic membrane proteins. About 80% of the proteins were recovered in the hydrophobic phase of the Triton X-114/H2O phase system and the remaining 20% in the aqueous phase. SDS-PAGE showed that the integral membrane polypeptides of the plasma membrane were dominated by 52, 49, 33, 29, 25 and 17 kilodalton polypeptides, while the major peripheral plasma membrane polypeptide was 41 kilodaltons. Different wash treatments of the... (More)
Plasma membrane vesicles of high purity were isolated from spinach (Spinacia oleracea) leaves by aqueous polymer 2-phase partition and analyzed with respect to their protein organization. This was done by Triton X-114 fractionation which separates integral, hydrophobic, membrane-spanning proteins from peripheral, hydrophilic membrane proteins. About 80% of the proteins were recovered in the hydrophobic phase of the Triton X-114/H2O phase system and the remaining 20% in the aqueous phase. SDS-PAGE showed that the integral membrane polypeptides of the plasma membrane were dominated by 52, 49, 33, 29, 25 and 17 kilodalton polypeptides, while the major peripheral plasma membrane polypeptide was 41 kilodaltons. Different wash treatments of the right-side-out plasma membrane vesicles suggest that the peripheral proteins are largely localized at the inner, cytoplasmic surface of the plasma membrane. Thus, Triton X-114 fractionation of plasma membranes should be useful for the functional identification of plasma membrane proteins as well as a first step in protein isolation. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant Physiology and Biochemistry
volume
27
pages
169 - 174
publisher
Elsevier
ISSN
1873-2690
language
English
LU publication?
yes
id
a1ca4a51-19d8-4ba5-95b0-ed5d8ef9a334
date added to LUP
2019-06-20 17:08:12
date last changed
2020-12-29 13:48:10
@article{a1ca4a51-19d8-4ba5-95b0-ed5d8ef9a334,
  abstract     = {{Plasma membrane vesicles of high purity were isolated from spinach (Spinacia oleracea) leaves by aqueous polymer 2-phase partition and analyzed with respect to their protein organization. This was done by Triton X-114 fractionation which separates integral, hydrophobic, membrane-spanning proteins from peripheral, hydrophilic membrane proteins. About 80% of the proteins were recovered in the hydrophobic phase of the Triton X-114/H2O phase system and the remaining 20% in the aqueous phase. SDS-PAGE showed that the integral membrane polypeptides of the plasma membrane were dominated by 52, 49, 33, 29, 25 and 17 kilodalton polypeptides, while the major peripheral plasma membrane polypeptide was 41 kilodaltons. Different wash treatments of the right-side-out plasma membrane vesicles suggest that the peripheral proteins are largely localized at the inner, cytoplasmic surface of the plasma membrane. Thus, Triton X-114 fractionation of plasma membranes should be useful for the functional identification of plasma membrane proteins as well as a first step in protein isolation.}},
  author       = {{Kjellbom, Per and Larsson, Christer and Rochester, Colin P. and Andersson, Bertil}},
  issn         = {{1873-2690}},
  language     = {{eng}},
  pages        = {{169--174}},
  publisher    = {{Elsevier}},
  series       = {{Plant Physiology and Biochemistry}},
  title        = {{Integral and peripheral proteins of the spinach leaf plasma membrane.}},
  volume       = {{27}},
  year         = {{1989}},
}